1996
More Sm snRNAs from Vertebrate Cells
Yu Y, Tarn W, Yario T, Steitz J. More Sm snRNAs from Vertebrate Cells. Experimental Cell Research 1996, 229: 276-281. PMID: 8986610, DOI: 10.1006/excr.1996.0372.Peer-Reviewed Original Research
1992
Site-specific cross-linking of mammalian U5 snRNP to the 5' splice site before the first step of pre-mRNA splicing.
Wyatt JR, Sontheimer EJ, Steitz JA. Site-specific cross-linking of mammalian U5 snRNP to the 5' splice site before the first step of pre-mRNA splicing. Genes & Development 1992, 6: 2542-2553. PMID: 1340469, DOI: 10.1101/gad.6.12b.2542.Peer-Reviewed Original ResearchConceptsSplice siteMRNA splicingATP-dependent interactionHeLa nuclear extractsU5 snRNPMRNA substratesSplice site regionProtein factorsU5 snRNANucleotides upstreamU6 snRNAPre-mRNANuclear extractsSplicingSite regionLoop sequenceCross-link formationSnRNASplicing conditionsWatson-Crick complementarityCross-linking strategyU1Selective photoactivationSnRNPMammalianInteractions of Small Nuclear RNA's with Precursor Messenger RNA During in Vitro Splicing
Wassarman D, Steitz J. Interactions of Small Nuclear RNA's with Precursor Messenger RNA During in Vitro Splicing. Science 1992, 257: 1918-1925. PMID: 1411506, DOI: 10.1126/science.1411506.Peer-Reviewed Original ResearchConceptsPrecursor messenger RNASmall nuclear RNANuclear RNASplice siteMessenger RNAPrecursor messenger RNA splicingU6 small nuclear RNAMessenger RNA splicingHeLa nuclear extractsExcised lariat intronSmall RNAsRNA splicingLariat intronIntron sequencesVitro splicingNuclear extractsCleavage eventsSplicingRNAU5Branch siteFirst cleavage eventU6U2U1
1984
U4 and U6 RNAs coexist in a single small nuclear ribonucleoprotein particle
Hashimoto C, Steitz J. U4 and U6 RNAs coexist in a single small nuclear ribonucleoprotein particle. Nucleic Acids Research 1984, 12: 3283-3293. PMID: 6201826, PMCID: PMC318745, DOI: 10.1093/nar/12.7.3283.Peer-Reviewed Original ResearchConceptsSmall nuclear ribonucleoproteinU6 RNARNA complexIntact small nuclear ribonucleoproteinRNA/RNA complexesHuman small nuclear ribonucleoproteinsSmall nuclear ribonucleoprotein particleRNA-RNA interactionsNuclear ribonucleoprotein particleSequence complementarityMammalian cellsNuclear ribonucleoproteinRibonucleoprotein particleSnRNP particlesRNABiological implicationsPolyacrylamide gelsProteinase KU4ComplexesSodium dodecyl sulfateRibonucleoproteinDodecyl sulfateU5U1Autoantibodies to the U2 small nuclear ribonucleoprotein in a patient with scleroderma-polymyositis overlap syndrome.
Mimori T, Hinterberger M, Pettersson I, Steitz JA. Autoantibodies to the U2 small nuclear ribonucleoprotein in a patient with scleroderma-polymyositis overlap syndrome. Journal Of Biological Chemistry 1984, 259: 560-565. PMID: 6231285, DOI: 10.1016/s0021-9258(17)43698-2.Peer-Reviewed Original ResearchConceptsU2 small nuclear ribonucleoproteinSmall nuclear ribonucleoproteinNuclear ribonucleoproteinU2 small nuclear RNASm small nuclear ribonucleoproteinsU6 small nuclear ribonucleoproteinProtein blot analysisSmall nuclear RNAScleroderma-polymyositis overlap syndromeU1 small nuclear ribonucleoproteinHeLa cell extractsRNA speciesNuclear RNAB proteinCell extractsProtein bandsBlot analysisOverlap syndromeRibonucleoproteinProteinAnti-Sm antibodiesU1InsectsRNAPolypeptide