1993
Rare scleroderma autoantibodies to the U11 small nuclear ribonucleoprotein and to the trimethylguanosine cap of U small nuclear RNAs.
Gilliam AC, Steitz JA. Rare scleroderma autoantibodies to the U11 small nuclear ribonucleoprotein and to the trimethylguanosine cap of U small nuclear RNAs. Proceedings Of The National Academy Of Sciences Of The United States Of America 1993, 90: 6781-6785. PMID: 8341699, PMCID: PMC47016, DOI: 10.1073/pnas.90.14.6781.Peer-Reviewed Original ResearchConceptsSmall nuclear ribonucleoprotein particleTrimethylguanosine capLow-abundance membersSmall nuclear RNASmall nuclear ribonucleoproteinNuclear ribonucleoprotein particleGlycerol gradient fractionsU RNAU11 small nuclear ribonucleoproteinsSnRNP complexTargeted degradationNuclear RNANuclear ribonucleoproteinRibonucleoprotein particleNuclear extractsSm classProtein componentsHeLa cellsRNPGradient fractionsRNAProteinScleroderma seraRibonucleoproteinCosediments
1989
The mammalian analogue of the yeast PRP8 splicing protein is present in the U4/5/6 small nuclear ribonucleoprotein particle and the spliceosome.
Pinto AL, Steitz JA. The mammalian analogue of the yeast PRP8 splicing protein is present in the U4/5/6 small nuclear ribonucleoprotein particle and the spliceosome. Proceedings Of The National Academy Of Sciences Of The United States Of America 1989, 86: 8742-8746. PMID: 2479028, PMCID: PMC298364, DOI: 10.1073/pnas.86.22.8742.Peer-Reviewed Original ResearchConceptsSmall nuclear ribonucleoproteinU5 small nuclear ribonucleoproteinHeLa cell nuclear extractsSmall nuclear ribonucleoprotein particleCell nuclear extractsAnti-trimethylguanosine antibodyNuclear ribonucleoprotein particleAffinity-purified spliceosomesSplicing proteinsMammalian proteinsYeast proteinsSnRNP complexMRNA splicingSplicing extractsRibonucleoprotein particleNuclear ribonucleoproteinMammalian analogueNuclear extractsSm classGradient fractionationSm epitopesProteinSpliceosomeProtein reactivePrp8
1983
The U1 small nuclear RNA-protein complex selectively binds a 5′ splice site in vitro
Mount S, Pettersson I, Hinterberger M, Karmas A, Steitz J. The U1 small nuclear RNA-protein complex selectively binds a 5′ splice site in vitro. Cell 1983, 33: 509-518. PMID: 6190573, DOI: 10.1016/0092-8674(83)90432-4.Peer-Reviewed Original ResearchConceptsRNA-protein complexesSplice siteU1 snRNPGlobin RNAPartial proteinase K digestionU1 snRNPsMouse beta-globin geneBacteriophage T7 RNA polymeraseEucaryotic cell nucleusMessenger RNA splicingT7 RNA polymeraseRecombinant DNA clonesBeta-globin geneT1 ribonuclease digestionSplice site sequencesSmall intronsSnRNP proteinsRNA splicingSnRNP complexDNA clonesRNA polymeraseSnRNPSite sequenceRibonuclease digestionProteinase K digestion