2023
Novel perfluoroalkyl substances (PFAS) discovered in whole blood using automated non-targeted analysis of dried blood spots
Koelmel J, Lin E, Parry E, Stelben P, Rennie E, Godri Pollitt K. Novel perfluoroalkyl substances (PFAS) discovered in whole blood using automated non-targeted analysis of dried blood spots. The Science Of The Total Environment 2023, 883: 163579. PMID: 37100129, PMCID: PMC10247435, DOI: 10.1016/j.scitotenv.2023.163579.Peer-Reviewed Original ResearchConceptsBlood spotsNovel perfluoroalkyl substancesHuman bloodVenous blood drawBlood spot cardsBlood spot samplesPerfluoroalkyl substancesPrenatal exposureBlood drawEnvironmental epidemiology studiesEpidemiology studiesFalse negative rateBloodLow false negative rateInvasive collection techniquesWhole bloodVulnerable populationsLiquid chromatography-high resolution tandem mass spectrometryUnderstanding of exposureSpot samplesSpot cardsHigh-resolution tandem mass spectrometryExposurePerfluoroalkyl ether carboxylic acidsScreening
2019
Re-modeling of foliar membrane lipids in a seagrass allows for growth in phosphorus-deplete conditions
Koelmel J, Campbell J, Guingab-Cagmat J, Meke L, Garrett T, Stingl U. Re-modeling of foliar membrane lipids in a seagrass allows for growth in phosphorus-deplete conditions. PLOS ONE 2019, 14: e0218690. PMID: 31774814, PMCID: PMC6880972, DOI: 10.1371/journal.pone.0218690.Peer-Reviewed Original ResearchConceptsSulfoquinovosyl diacylglycerolCertain speciesPhosphorus-depleted conditionsLeaves of plantsGalactolipid digalactosyldiacylglycerolMost speciesHigh-resolution tandem mass spectrometryLeaf membranesPhosphorus contentMembrane lipidsSeagrassSpeciesEnvironmental availabilityDiacylglyceryltrimethylhomoserineDigalactosyldiacylglycerolLeavesElemental availabilityTandem mass spectrometryStructural differencesLiquid chromatography-high resolution tandem mass spectrometryGlucuronosyldiacylglycerolMass spectrometryTurtlegrassLow phosphorus contentPlantsLipidomics for wildlife disease etiology and biomarker discovery: a case study of pansteatitis outbreak in South Africa
Koelmel J, Ulmer C, Fogelson S, Jones C, Botha H, Bangma J, Guillette T, Luus-Powell W, Sara J, Smit W, Albert K, Miller H, Guillette M, Olsen B, Cochran J, Garrett T, Yost R, Bowden J. Lipidomics for wildlife disease etiology and biomarker discovery: a case study of pansteatitis outbreak in South Africa. Metabolomics 2019, 15: 38. PMID: 30838461, PMCID: PMC11005104, DOI: 10.1007/s11306-019-1490-9.Peer-Reviewed Original ResearchConceptsApplication of lipidomicsDiverse biological rolesWildlife studiesPossible disease mechanismsClasses of lipidsHigh-resolution tandem mass spectrometryLoskop DamBiological roleMozambique tilapiaCell deathMortality eventsPansteatitisLipidomicsOxidative damageDisease mechanismsAdipose tissuePromising biomarker candidatesBiomarker discoveryDisease etiologyTilapiaTandem mass spectrometryCeramideAquatic lifeLipidomeBiomarker candidates
2018
Examining heat treatment for stabilization of the lipidome
Koelmel J, Jones C, Ulmer C, Garrett T, Yost R, Schock T, Bowden J. Examining heat treatment for stabilization of the lipidome. Bioanalysis 2018, 10: 291-305. PMID: 29451398, DOI: 10.4155/bio-2017-0209.Peer-Reviewed Original ResearchConceptsLiquid chromatography-high resolution tandem mass spectrometryEnzymatic productResolution tandem mass spectrometryHigh-resolution tandem mass spectrometryLipid biomarker researchTandem mass spectrometryLipid-based biomarkersEnzyme stabilizerMass spectrometryLipid identificationHeat treatmentSample weighingFlash freezingHeat-treated samplesFlash-frozen samples
2017
Expanding Lipidome Coverage Using LC-MS/MS Data-Dependent Acquisition with Automated Exclusion List Generation
Koelmel J, Kroeger N, Gill E, Ulmer C, Bowden J, Patterson R, Yost R, Garrett T. Expanding Lipidome Coverage Using LC-MS/MS Data-Dependent Acquisition with Automated Exclusion List Generation. Journal Of The American Society For Mass Spectrometry 2017, 28: 908-917. PMID: 28265968, PMCID: PMC5408749, DOI: 10.1007/s13361-017-1608-0.Peer-Reviewed Original ResearchHR-MS/MS spectraUltra-high performance liquid chromatographyResolution tandem mass spectrometryHigh-resolution tandem mass spectrometryPositive ion modeField of lipidomicsTandem mass spectrometryMS/MSIon modePerformance liquid chromatographyMS spectraLC-MS/MS dataMass spectrometryChromatographic elutionSequential injectionLiquid chromatographyTrace speciesMS dataUnique precursorDependent acquisitionBiomoleculesBiological systemsNumber of precursors