2014
Reticuloendothelial Saturation Dictates the Development of RBC Resistance to Antibody-Mediated Clearance Following Incompatible Transfusion
Arthur C, Mener A, Patel S, Rodrigues L, Xue C, Nickel R, Zimring J, Hendrickson J, Stowell S. Reticuloendothelial Saturation Dictates the Development of RBC Resistance to Antibody-Mediated Clearance Following Incompatible Transfusion. Blood 2014, 124: 1559. DOI: 10.1182/blood.v124.21.1559.1559.Peer-Reviewed Original ResearchIncompatible RBC transfusionRed blood cellsRBC transfusionInitial transfusionRBC clearanceAntigen modulationOld red blood cellsAntibody-mediated removalFlow cytometric analysisHOD antigenIncompatible transfusionsDetectable antigenRBC ageReticuloendothelial systemCytometric analysisPreferential clearanceAntibody-Mediated ClearanceRBC-specific antibodiesAntibody-opsonized cellsPhagocytic removalTransfused cellsC57BL/6 recipientsAntigen levelsFVB miceRecipient miceCD8+ T Cells Mediate Antibody-Independent Platelet Clearance Following Transfusion
Patel S, Arthur C, Rodrigues L, Xue C, Zimring J, Hendrickson J, Stowell S. CD8+ T Cells Mediate Antibody-Independent Platelet Clearance Following Transfusion. Blood 2014, 124: 596. DOI: 10.1182/blood.v124.21.596.596.Peer-Reviewed Original ResearchPlatelet-rich plasmaMajor histocompatibility complexNK cell depletionT cellsPlatelet clearanceC57BL/6 recipientsPlatelet transfusionsNK cellsCellular immunityCell depletionAnti-MHC antibodiesSubsequent blood transfusionDepletion of CD8Solid organ allograftsAnti-platelet antibodiesCD8 T cellsClearance of plateletsPlatelet transfusion therapyFlow cytometric analysisLater time pointsC57BL/6 donorsMHC alloantibodiesNK1.1 antibodyOrgan allograftsBlood transfusion
2013
KEL RBC Transfusion Induces IgG Anti-KEL Antibodies Independent Of CD4 T Cells
Stowell S, Girard-Pierce K, Arthur C, Smith N, Zimring J, Hendrickson J. KEL RBC Transfusion Induces IgG Anti-KEL Antibodies Independent Of CD4 T Cells. Blood 2013, 122: 41. DOI: 10.1182/blood.v122.21.41.41.Peer-Reviewed Original ResearchCD4 T cellsMHC class IIRBC alloantibody formationRBC transfusionT cellsKEL RBCsIsotype controlAlloantibody formationC57BL/6 recipientsRBC alloantibodiesRBC alloantigensClass IICD-4 T-cellsCD4 T-cell depletionRed blood cell transfusionCD4 T cell helpMHC class II antigensChronic transfusion therapyBlood cell transfusionT-cell depletionHemolytic transfusion reactionsIsotype control antibodyClass II antigensT cell helpMHC antigen presentationTransfusion Of RBCs With Low-Density KEL Induces Tolerance To The KEL Antigen
Stowell S, Arthur C, Smith N, Girard-Pierce K, Zimring J, Hendrickson J. Transfusion Of RBCs With Low-Density KEL Induces Tolerance To The KEL Antigen. Blood 2013, 122: 1160. DOI: 10.1182/blood.v122.21.1160.1160.Peer-Reviewed Original ResearchKEL RBCsRBC transfusionAntibody formationKEL antigenDetectable IgMRBC alloimmunizationWestern blot analysisC57BL/6 recipientsAntigen densityDay 3RBC antigensDays post transfusionTransfusion of RBCsSubsequent responseBlot analysisFlow cytometric analysisAnalysis of serumCompatible RBCsRBC alloantigensRepeat transfusionPost transfusionAntigen levelsImmunological outcomesRBC exposureSpecific alloantibodies
2011
A Novel Murine Model of Pregnancy and Transfusion Induced Anti-Kell RBC Alloimmunization
Smith N, Henry K, Zimring J, Hendrickson J. A Novel Murine Model of Pregnancy and Transfusion Induced Anti-Kell RBC Alloimmunization. Blood 2011, 118: 715. DOI: 10.1182/blood.v118.21.715.715.Peer-Reviewed Original ResearchNon-immunized mothersRBC alloimmunizationNovel murine modelGlycoprotein antibodiesC57BL/6 recipientsImmunized mothersNegative fetusMurine modelAnimal modelsC57BL/6 femalesMechanisms of immunizationRBC-specific expressionSubset of pregnanciesFlow cytometric crossmatchSubtype-specific antibodiesDays of lifeFirst animal modelAnti-Kell antibodiesPrior pregnancySubsequent transfusionsCytometric crossmatchRBC transfusionAntibody titersRepeat pregnancyRBC antibodies
2010
Fresh Murine Red Blood Cells Abrogate the Enhanced Alloimmunogenicity of Stored Murine Red Blood Cells
Hendrickson J, Hod E, Spitalnik S, Zimring J. Fresh Murine Red Blood Cells Abrogate the Enhanced Alloimmunogenicity of Stored Murine Red Blood Cells. Blood 2010, 116: 663. DOI: 10.1182/blood.v116.21.663.663.Peer-Reviewed Original ResearchFresh red blood cellsStored red blood cellsMurine red blood cellsRed blood cellsOld red blood cellsBlood cellsMurine modelFlow cytometric crossmatchRandomized clinical studyRates of immunizationYoung red blood cellsAdditional control animalsCPDA-1Alloimmunization rateFVB controlsAlloantibody responsesAlloimmune responseC57BL/6 recipientsCytometric crossmatchPotential deleterious effectsImmunoregulatory propertiesWorse outcomesClinical dataClinical studiesAntigen expression