2024
A simple, field-applicable method to increase the infectivity of wild isolates of Plasmodium falciparum to mosquito vectors
Ouattara S, Hien D, Nao E, Paré P, Guissou E, Cohuet A, Morlais I, Yerbanga R, Dabiré K, Ouédraogo J, Mouline K, Lefèvre T. A simple, field-applicable method to increase the infectivity of wild isolates of Plasmodium falciparum to mosquito vectors. Malaria Journal 2024, 23: 135. PMID: 38711028, PMCID: PMC11075210, DOI: 10.1186/s12936-024-04969-0.Peer-Reviewed Original ResearchConceptsDirect membrane feeding assaysGametocyte densityHuman-to-mosquito transmissionDonor bloodLow gametocyte densitiesMembrane feeding assaysPlasmodium falciparum gametocytesTransmission-blocking interventionsWhole bloodInfection rateFalciparum gametocytesInfectious reservoirNaive serumAsymptomatic individualsMosquito infectionHuman donorsRemoval of plasmaEndemic countriesSerumPlasmodiumParasite isolatesInfectionBlood treatmentGametocytesPlasma volume
2013
Comparison of field-based xenodiagnosis and direct membrane feeding assays for evaluating host infectiousness to malaria vector Anopheles gambiae
Gouagna L, Yao F, Yameogo B, Dabiré R, Ouédraogo J. Comparison of field-based xenodiagnosis and direct membrane feeding assays for evaluating host infectiousness to malaria vector Anopheles gambiae. Acta Tropica 2013, 130: 131-139. PMID: 24262642, DOI: 10.1016/j.actatropica.2013.10.022.Peer-Reviewed Original ResearchConceptsMosquito bitesOocyst infectionDirect membrane feeding assaysInfectious mosquito bitesAnopheles mosquitoesMembrane feeding assaysMalaria-endemic areasLow gametocyte densitiesCross-sectional surveyGametocyte densityEndemic areasDay 7Classical microscopic examinationHost infectiousnessBlood smearsOocyst prevalenceXenodiagnosisHost skinMembrane feederInfectionInfectious individualsInfectiousnessMicroscopic examinationHuman subjectsChildren