2015
C/EBPβ (CEBPB) protein binding to the C/EBP|CRE DNA 8-mer TTGC|GTCA is inhibited by 5hmC and enhanced by 5mC, 5fC, and 5caC in the CG dinucleotide
Sayeed SK, Zhao J, Sathyanarayana BK, Golla JP, Vinson C. C/EBPβ (CEBPB) protein binding to the C/EBP|CRE DNA 8-mer TTGC|GTCA is inhibited by 5hmC and enhanced by 5mC, 5fC, and 5caC in the CG dinucleotide. Biochimica Et Biophysica Acta 2015, 1849: 583-589. PMID: 25779641, PMCID: PMC7857639, DOI: 10.1016/j.bbagrm.2015.03.002.Peer-Reviewed Original ResearchConceptsCG dinucleotideSequence-specific DNA bindingMammalian developmentTET dioxygenasesTranscription factorsDNA sequencesDNA bindingCircular dichroismElectrophoretic mobilityThermal denaturationMotifDinucleotideStructural differencesBindingDioxygenasesHomodimerEMSAOxidative productsProteinDNAChemical formsEBPSequenceCytosineDichroism
2014
Carboxylation of cytosine (5caC) in the CG dinucleotide in the E-box motif (CGCAG|GTG) increases binding of the Tcf3|Ascl1 helix-loop-helix heterodimer 10-fold
Golla JP, Zhao J, Mann IK, Sayeed SK, Mandal A, Rose RB, Vinson C. Carboxylation of cytosine (5caC) in the CG dinucleotide in the E-box motif (CGCAG|GTG) increases binding of the Tcf3|Ascl1 helix-loop-helix heterodimer 10-fold. Biochemical And Biophysical Research Communications 2014, 449: 248-255. PMID: 24835951, PMCID: PMC6258048, DOI: 10.1016/j.bbrc.2014.05.018.Peer-Reviewed Original Research