2023
Cellular protection from H2O2 toxicity by Fv-Hsp70: protection via catalase and gamma-glutamyl-cysteine synthase
Hino C, Chan G, Jordaan G, Chang S, Saunders J, Bashir M, Hansen J, Gera J, Weisbart R, Nishimura R. Cellular protection from H2O2 toxicity by Fv-Hsp70: protection via catalase and gamma-glutamyl-cysteine synthase. Cell Stress And Chaperones 2023, 28: 429-439. PMID: 37171750, PMCID: PMC10352194, DOI: 10.1007/s12192-023-01349-6.Peer-Reviewed Original ResearchMeSH KeywordsAdenosine TriphosphatasesCatalaseCysteine SynthaseHeat-Shock ProteinsHSP70 Heat-Shock ProteinsHumansHydrogen PeroxideNeuroblastomaRecombinant ProteinsConceptsHeat shock proteinsGamma-glutamylcysteine synthaseCellular protectionAcute oxidative stressLid domainProtein-binding domainsATPase domainCysteine synthaseOxidative stressCellular stressRecombinant proteinsSH-SY5Y cellsEndogenous heat shock proteinsShock proteinsCell deathProteinProtein catalaseHSP70H2O2 toxicityCellsSynthaseΜM concentrationsDomainCatalaseDirect response
2010
Recombinant Fv-Hsp70 Protein Mediates Neuroprotection After Focal Cerebral Ischemia in Rats
Zhan X, Ander BP, Liao IH, Hansen JE, Kim C, Clements D, Weisbart RH, Nishimura RN, Sharp FR. Recombinant Fv-Hsp70 Protein Mediates Neuroprotection After Focal Cerebral Ischemia in Rats. Stroke 2010, 41: 538-543. PMID: 20075343, PMCID: PMC2957177, DOI: 10.1161/strokeaha.109.572537.Peer-Reviewed Original ResearchConceptsFocal cerebral ischemiaCerebral ischemiaInfarction volumeSensorimotor functionSaline-treated control groupIntraluminal suture techniqueExperimental ischemic strokeExperimental stroke modelsMiddle cerebral arteryOnset of ischemiaInfarct volumeIschemic brainIschemic strokeCerebral arteryFocal ischemiaControl brainsStroke modelTail veinIschemiaControl groupSuture techniqueWestern blotEndogenous Hsp70RatsBrain
2006
Antibody-mediated Hsp70 protein therapy
Hansen JE, Sohn W, Kim C, Chang SS, Huang NC, Santos DG, Chan G, Weisbart RH, Nishimura RN. Antibody-mediated Hsp70 protein therapy. Brain Research 2006, 1088: 187-196. PMID: 16630585, DOI: 10.1016/j.brainres.2006.03.025.Peer-Reviewed Original ResearchMeSH KeywordsAnimalsAnimals, NewbornBlotting, WesternCell SurvivalCells, CulturedCerebral CortexChlorocebus aethiopsDose-Response Relationship, DrugHSP70 Heat-Shock ProteinsHumansHydrogen PeroxideImmunoglobulin Variable RegionNeuronsOxidantsProtein TransportRatsRats, WistarRecombinant Fusion ProteinsTransduction, GeneticTransfectionConceptsPrimary rat cortical neuronsRat cortical neuronsCortical neuronsIntracellular HSP70 levelsCOS-7 cellsSingle-chain Fv fragmentAnti-DNA antibodiesProtein therapyIntracellular HSP70Stressful stimuliFv fragmentMAb 3E10Hsp70 levelsNeuronsTherapyProtein transduction domainSubsequent exposureCellsHuman diseasesNovel protein transduction domainFirst evidenceTransduction domainLiving cellsHSP70Fusion protein