2023
Cryo-EM reveals how the mastigoneme assembles and responds to environmental signal changes
Wang Y, Yang J, Hu F, Yang Y, Huang K, Zhang K. Cryo-EM reveals how the mastigoneme assembles and responds to environmental signal changes. Journal Of Cell Biology 2023, 222: e202301066. PMID: 37882754, PMCID: PMC10602792, DOI: 10.1083/jcb.202301066.Peer-Reviewed Original ResearchConceptsSingle-particle cryo-electron microscopy structureCryo-electron microscopy structureNon-polar filamentsImmunoglobulin-like domainsDistal regionPolyproline II helixMicroscopy structureChlamydomonas reinhardtiiFlagellar motilityVaried environmental conditionsCryo-EMCell swimmingMotility controlEnvironmental responsesDisulfide bondsEnvironmental conditionsSushi domainMastigonemesThread-like structuresRedox shiftSignal sensingPotential roleProtistsReinhardtiiMST1
2022
Cryo-EM structure of an active central apparatus
Han L, Rao Q, Yang R, Wang Y, Chai P, Xiong Y, Zhang K. Cryo-EM structure of an active central apparatus. Nature Structural & Molecular Biology 2022, 29: 472-482. PMID: 35578022, PMCID: PMC9113940, DOI: 10.1038/s41594-022-00769-9.Peer-Reviewed Original ResearchConceptsCentral apparatusDiverse cellular activitiesKinesin-like proteinCryo-EM structureArmadillo repeat proteinsCryo-electron microscopyHigh-resolution structuresEukaryotic speciesProtein subunitsMotile ciliaBridge proteinsPair of microtubulesRegulatory roleCellular activitiesProteinDynamic conformational behaviorCiliary motilityCiliaCiliary beatingStructural frameworkConformational behaviorSubunitsMicrotubulesRegulatorSpecies