2016
Replication fork passage drives asymmetric dynamics of a critical nucleoid‐associated protein in Caulobacter
Arias‐Cartin R, Dobihal GS, Campos M, Surovtsev IV, Parry B, Jacobs‐Wagner C. Replication fork passage drives asymmetric dynamics of a critical nucleoid‐associated protein in Caulobacter. The EMBO Journal 2016, 36: 301-318. PMID: 28011580, PMCID: PMC5286365, DOI: 10.15252/embj.201695513.Peer-Reviewed Original ResearchConceptsNucleoid-associated proteinsDNA replicationNovel nucleoid-associated proteinGene expressionReplication fork passageGlobal gene expressionCell cycle regulationDNA-binding activityCell cycle progressionChromosome dynamicsFork passageChromosome segregationPleiotropic defectsChromosomal biasCaulobacter crescentusAsymmetric localizationReplication forksCellular functionsCycle regulationGenomic techniquesQuantitative cell imagingCell divisionGapRCycle progressionCell cycle
2015
Oufti: an integrated software package for high‐accuracy, high‐throughput quantitative microscopy analysis
Paintdakhi A, Parry B, Campos M, Irnov I, Elf J, Surovtsev I, Jacobs-Wagner C. Oufti: an integrated software package for high‐accuracy, high‐throughput quantitative microscopy analysis. Molecular Microbiology 2015, 99: 767-777. PMID: 26538279, PMCID: PMC4752901, DOI: 10.1111/mmi.13264.Peer-Reviewed Original ResearchConceptsSingle-cell studiesOpen-source software packageGraphical user interfaceComplex subcellular organizationSoftware packageImage analysisUser interfaceMassive datasetsCellular functionsSubcellular organizationBacterial researchHigh-throughput analysisTouching cellsMicrobial cellsSubpixel precisionInteractive modulesComputational skillsComputational solutionPost-processing analysisPhenotypic variabilityFluorescence signalQuantitative microscopy analysisCell morphologyAutomated MeasurementConfluent samples