2020
Clinical utilization of species‐specific immunoassays for identification of Staphylococcus aureus and Streptococcus agalactiae in orthopedic infections
Sulovari A, Ninomiya MJ, Beck CA, Ricciardi BF, Ketonis C, Mesfin A, Kaplan NB, Soin SP, McDowell SM, Mahmood B, Daiss JL, Schwarz EM, Oh I. Clinical utilization of species‐specific immunoassays for identification of Staphylococcus aureus and Streptococcus agalactiae in orthopedic infections. Journal Of Orthopaedic Research® 2020, 39: 2141-2150. PMID: 33274775, PMCID: PMC8175449, DOI: 10.1002/jor.24935.Peer-Reviewed Original ResearchConceptsPeripheral blood mononuclear cellsFracture-related infectionMusculoskeletal infectionsOrthopedic infectionsHigh diagnostic potentialCultured peripheral blood mononuclear cellsDiagnostic potentialDeep musculoskeletal infectionSignificant patient morbidityBlood mononuclear cellsDiabetic foot ulcersAntigen-specific IgGPeriprosthetic joint infectionStaphylococcus aureusInfection patientsSeptic arthritisSurgical treatmentCurrent diagnostic methodsFoot ulcersJoint infectionAntibody titersMononuclear cellsPatient morbidityPolymicrobial infectionsAntibiotic useSpecies-Specific Immunoassay Aids Identification of Pathogen and Tracks Infectivity in Foot Infection
Hao SP, Masters EA, Ninomiya MJ, Beck CA, Schwarz EM, Daiss JL, Oh I. Species-Specific Immunoassay Aids Identification of Pathogen and Tracks Infectivity in Foot Infection. Foot & Ankle International 2020, 42: 363-372. PMID: 33161780, PMCID: PMC7946706, DOI: 10.1177/1071100720965136.Peer-Reviewed Original ResearchConceptsFoot infectionsHost immune responseWound healing statusImmune responseHealing statusFoot ulcer patientsProspective comparative studyCulture-positive patientsCulture-negative patientsSpecific serum antibodiesDominant pathogenAnalysis of serumConventional bacterial cultureUlcer patientsMusculoskeletal infectionsSurgical interventionAntibody titersSerum antibodiesTherapeutic responseInfection diagnosisSpecies-specific immunoassaySurgeon's decisionCulture resultsInfectionLevel IISpecies-Specific Immunoassay for Diagnosis and Tracking Treatment Response in Foot and Ankle Infection
Oh I, Hao S, Waldman O, Holleran A, Daiss J. Species-Specific Immunoassay for Diagnosis and Tracking Treatment Response in Foot and Ankle Infection. Foot & Ankle Orthopaedics 2020, 5: 2473011420s00372. PMCID: PMC8702934, DOI: 10.1177/2473011420s00372.Peer-Reviewed Original ResearchAnkle infectionWound healing statusHealing statusDFU patientsOngoing infectionTreatment responseS. aureusDiabetic foot ulcer patientsFellowship-trained orthopedic footFoot ulcer patientsCharacteristic curve analysisDiagnostic toolSpecies-specific immunoassayReliable diagnostic toolOrthopaedic FootSpecific diagnostic toolsUlcer patientsAntibiotic therapyClinical resolutionSurgical treatmentClinical outcomesInitial presentationAntibody titersSerum antibodiesAnkle surgeons
2018
Tracking Anti-Staphylococcus aureus Antibodies Produced In Vivo and Ex Vivo during Foot Salvage Therapy for Diabetic Foot Infections Reveals Prognostic Insights and Evidence of Diversified Humoral Immunity
Oh I, Muthukrishnan G, Ninomiya MJ, Brodell JD, Smith BL, Lee CC, Gill SR, Beck CA, Schwarz EM, Daiss JL. Tracking Anti-Staphylococcus aureus Antibodies Produced In Vivo and Ex Vivo during Foot Salvage Therapy for Diabetic Foot Infections Reveals Prognostic Insights and Evidence of Diversified Humoral Immunity. Infection And Immunity 2018, 86: 10.1128/iai.00629-18. PMID: 30275008, PMCID: PMC6246899, DOI: 10.1128/iai.00629-18.Peer-Reviewed Original ResearchConceptsDiabetic foot infectionsIgG levelsDFI patientsSalvage therapyFoot infectionsCultured peripheral blood mononuclear cellsPeripheral blood mononuclear cellsBlood mononuclear cellsSimilar diagnostic potentialAggressive treatmentAntibody titersHumoral immunityMononuclear cellsPrognostic insightsPrognostic potentialCommon pathogensEtiologic agentMultivariate analysisClinical decisionEx vivoMultiplex immunoassayWound samplesInfectionSerumAureus antibody