The Complete Genome Sequences, Unique Mutational Spectra, and Developmental Potency of Adult Neurons Revealed by Cloning
Hazen JL, Faust GG, Rodriguez AR, Ferguson WC, Shumilina S, Clark RA, Boland MJ, Martin G, Chubukov P, Tsunemoto RK, Torkamani A, Kupriyanov S, Hall IM, Baldwin KK. The Complete Genome Sequences, Unique Mutational Spectra, and Developmental Potency of Adult Neurons Revealed by Cloning. Neuron 2016, 89: 1223-1236. PMID: 26948891, PMCID: PMC4795965, DOI: 10.1016/j.neuron.2016.02.004.Peer-Reviewed Original ResearchMeSH KeywordsAge FactorsAnimalsAnimals, NewbornCadherin Related ProteinsCadherinsCell DivisionCloning, MolecularDNA Transposable ElementsEmbryo, MammalianFemaleHumansKi-67 AntigenMiceMice, TransgenicMicrosatellite RepeatsMutationNerve Tissue ProteinsNeuronsNuclear Transfer TechniquesOlfactory BulbOocytesSequence Analysis, DNAConceptsCell type diversificationComplete genome sequenceMobile element insertionsNuclear transfer methodWhole-genome sequencingNeuronal genomeGene-disrupting mutationsNeuronal mutationsGenome sequenceUnique mutational spectrumDevelopmental potencyComprehensive mutation detectionElement insertionsGenomic mutationsRecurrent rearrangementsNovel mechanismUnique mutationsMutationsSomatic mutationsGene biasGenomeAdult neuronsMutational spectrumFertile miceMutation detectionMosaic Copy Number Variation in Human Neurons
McConnell MJ, Lindberg MR, Brennand KJ, Piper JC, Voet T, Cowing-Zitron C, Shumilina S, Lasken RS, Vermeesch JR, Hall IM, Gage FH. Mosaic Copy Number Variation in Human Neurons. Science 2013, 342: 632-637. PMID: 24179226, PMCID: PMC3975283, DOI: 10.1126/science.1243472.Peer-Reviewed Original ResearchConceptsCopy number variationsHiPSC-derived neuronsSingle-cell genomic approachesNumber variationsDNA copy number variationsSingle-cell sequencingHuman neuronsLarge copy number variationsStem cell linesNeural progenitor cellsNovo copy-number variationsPluripotent stem cell lineAneuploid neuronsGenomic approachesDe novo copy-number variationsSubchromosomal copy number variationsAberrant genomesFrontal cortex neuronsLarge deletionsProgenitor cellsCell linesSubset of neuronsEuploid neuronsDeletionMultiple alterations