Featured Publications
Genome Sequencing of Mouse Induced Pluripotent Stem Cells Reveals Retroelement Stability and Infrequent DNA Rearrangement during Reprogramming
Quinlan AR, Boland MJ, Leibowitz ML, Shumilina S, Pehrson SM, Baldwin KK, Hall IM. Genome Sequencing of Mouse Induced Pluripotent Stem Cells Reveals Retroelement Stability and Infrequent DNA Rearrangement during Reprogramming. Cell Stem Cell 2011, 9: 366-373. PMID: 21982236, PMCID: PMC3975295, DOI: 10.1016/j.stem.2011.07.018.Peer-Reviewed Original ResearchMeSH KeywordsAnimalsBase SequenceCell LineageCellular ReprogrammingChimeraDNA Copy Number VariationsFalse Negative ReactionsGene RearrangementGene SilencingGenomeGenomic InstabilityHumansInduced Pluripotent Stem CellsMiceMolecular Sequence DataMutagenesis, InsertionalOrgan SpecificityRetroelementsSequence Analysis, DNAConceptsPluripotent stem cellsClasses of SVsPaired-end DNA sequencingStem cellsGenomic structural variationMouse Induced Pluripotent Stem CellsStructural variationsDNA copy number variationsEmbryonic stem cellsMost iPSC linesMouse iPSC linesIPSC linesInduced pluripotent stem cellsCopy number variationsGenome stabilityGene-disrupting mutationsRecent microarray studiesDNA rearrangementsGenome sequencingSpontaneous mutationsMicroarray studiesDeleterious genetic mutationsNumber variationsDNA sequencingComplex rearrangements
2017
Using Cloning to Amplify Neuronal Genomes for Whole-Genome Sequencing and Comprehensive Mutation Detection and Validation
Hazen J, Duran M, Smith R, Rodriguez A, Martin G, Kupriyanov S, Hall I, Baldwin K. Using Cloning to Amplify Neuronal Genomes for Whole-Genome Sequencing and Comprehensive Mutation Detection and Validation. Neuromethods 2017, 131: 163-185. DOI: 10.1007/978-1-4939-7280-7_9.Peer-Reviewed Original ResearchNeuronal genomeSomatic cell nuclear transferEmbryonic cell lineClasses of mutationsEmbryonic stem cellsSequencing-based approachesCell nuclear transferSomatic mutationsWhole-genome sequencingNext-generation sequencingMutation detectionSomatic cellsBioinformatics methodsComprehensive mutation detectionNuclear transferIndividual mutationsGenomeCell typesStem cellsNew mutationsMutationsSingle cellsSequencingCell linesFalse positive calls