Excision of a Viral Reprogramming Cassette by Delivery of Synthetic Cre mRNA
Loh Y, Yang JC, De Los Angeles A, Guo C, Cherry A, Rossi DJ, Park I, Daley GQ. Excision of a Viral Reprogramming Cassette by Delivery of Synthetic Cre mRNA. Current Protocols In Stem Cell Biology 2012, 21: 4a.5.1-4a.5.16. PMID: 22605648, PMCID: PMC3397830, DOI: 10.1002/9780470151808.sc04a05s21.Peer-Reviewed Original ResearchConceptsPluripotent stem cellsTransgene-free methodsHuman iPS cellsResidual transgene expressionPatient-specific induced pluripotent stem cellsIPS cellsInduced pluripotent stem cellsStem cellsTransgene expressionFree humanHuman OCT4MRNA transfectionDrug screeningProtein transductionCre mRNADifferentiation potentialCell therapySingle vectorLow efficiencyCassetteRetroviral transfectionCre recombinaseEfficiencyTransfectionExperimental generationHuman induced pluripotent stem cells and neurodegenerative disease
Jung YW, Hysolli E, Kim KY, Tanaka Y, Park IH. Human induced pluripotent stem cells and neurodegenerative disease. Current Opinion In Neurology 2012, 25: 125-130. PMID: 22357218, PMCID: PMC3786112, DOI: 10.1097/wco.0b013e3283518226.Peer-Reviewed Original ResearchConceptsNeurodegenerative diseasesReplacement therapyCell replacement therapyParkinson's diseasePositive therapeutic effectDisease modelsParkinson's disease modelAmyotrophic lateral sclerosisNeuron-specific transcription factorSpinal muscular atrophyStem cellsDrug screeningDopaminergic neuronsIntermediate cell typeTherapeutic effectEffective treatmentLateral sclerosisDisease-specific iPSCsMuscular atrophyAlzheimer's diseaseNeurodegenerative disordersCellular therapyDiseaseTherapyHuman induced pluripotent stem cells