2015
dNP2 is a blood–brain barrier-permeable peptide enabling ctCTLA-4 protein delivery to ameliorate experimental autoimmune encephalomyelitis
Lim S, Kim WJ, Kim YH, Lee S, Koo JH, Lee JA, Yoon H, Kim DH, Park HJ, Kim HM, Lee HG, Yun Kim J, Lee JU, Hun Shin J, Kyun Kim L, Doh J, Kim H, Lee SK, Bothwell AL, Suh M, Choi JM. dNP2 is a blood–brain barrier-permeable peptide enabling ctCTLA-4 protein delivery to ameliorate experimental autoimmune encephalomyelitis. Nature Communications 2015, 6: 8244. PMID: 26372309, PMCID: PMC4579786, DOI: 10.1038/ncomms9244.Peer-Reviewed Original ResearchConceptsExperimental autoimmune encephalomyelitisMultiple sclerosisT cellsAutoimmune encephalomyelitisCytotoxic T-lymphocyte antigen-4T-lymphocyte antigen-4T helper 17 (Th17) cellsCNS inflammatory diseasesTherapeutic mouse modelsEffector T cellsHelper 17 cellsT helper 1Blood-brain barrierCentral nervous systemHuman T cellsHelper 1Antigen-4Inflammatory diseasesMouse modelNervous systemCurrent drugsResident cellsBrain tissueEffective agentCell-permeable peptide
2014
Cell membrane penetrating function of the nuclear localization sequence in human cytokine IL-1α
Koo J, Yoon H, Kim W, Lim S, Park H, Choi J. Cell membrane penetrating function of the nuclear localization sequence in human cytokine IL-1α. Molecular Biology Reports 2014, 41: 8117-8126. PMID: 25205122, DOI: 10.1007/s11033-014-3711-7.Peer-Reviewed Original ResearchConceptsNuclear localization sequenceLocalization sequenceCell penetrating peptideSpecific gene expressionCell membraneEnhanced green fluorescence proteinGreen fluorescence proteinHeparan sulfate interactionProtein delivery efficiencyMembrane penetration abilityGene expressionTAT-CPPFluorescence proteinCellular responsesPrecursor formAmino acidsIntracellular delivery efficiencyProteinDelivery peptidePenetrating peptideSequenceSulfate interactionInterleukin-1 alphaMembraneCells