2004
Three distinct mechanisms of HCO3− secretion in rat distal colon
Vidyasagar S, Rajendran VM, Binder HJ. Three distinct mechanisms of HCO3− secretion in rat distal colon. American Journal Of Physiology - Cell Physiology 2004, 287: c612-c621. PMID: 15308466, DOI: 10.1152/ajpcell.00474.2003.Peer-Reviewed Original ResearchConceptsRat distal colonShort-chain fatty acidsColonic mucosaDistal colonRat distal colonic mucosaDistal colonic mucosaCl- channel blockerSCFA/Channel blockersSerosal layerCrypt cellsCFTR blockerMicroM DIDSApical membrane anion channelApical membraneSecretionDependent secretionColonLumen ClSecretory mechanismMammalian colonBlockersMucosaStat techniqueDIDS
2001
Characterization of apical membrane Cl-dependent Na/H exchange in crypt cells of rat distal colon
Rajendran V, Geibel J, Binder H. Characterization of apical membrane Cl-dependent Na/H exchange in crypt cells of rat distal colon. AJP Gastrointestinal And Liver Physiology 2001, 280: g400-g405. PMID: 11171622, DOI: 10.1152/ajpgi.2001.280.3.g400.Peer-Reviewed Original ResearchConceptsRat distal colonNa/H exchangeCl- channel blockerDistal colonChannel blockersCrypt cellsIntracellular pH recoveryExchange inhibitor amilorideColonic epithelial cellsApical membrane vesiclesCrypt glandsHigh doseHOE 694NA depletionAcid loadExchange inhibitorInhibitor amiloridePH recoveryEpithelial cellsUptake 1NHE isoformsAnion exchange inhibitorBlockersColonNa uptake
1998
Role of Apical H-K Exchange and Basolateral K Channel in the Regulation of Intracellular pH in Rat Distal Colon Crypt Cells
Ikuma M, Binder H, Geibel J. Role of Apical H-K Exchange and Basolateral K Channel in the Regulation of Intracellular pH in Rat Distal Colon Crypt Cells. The Journal Of Membrane Biology 1998, 166: 205-212. PMID: 9843594, DOI: 10.1007/s002329900462.Peer-Reviewed Original ResearchMeSH KeywordsAnimalsAntiportersBariumCell PolarityColonEpithelial CellsH(+)-K(+)-Exchanging ATPaseHomeostasisHydrogen-Ion ConcentrationIntestinal MucosaIntracellular FluidIon TransportMaleOuabainPotassiumPotassium ChannelsPotassium-Hydrogen AntiportersRatsRats, Sprague-DawleySodium-Potassium-Exchanging ATPase
1997
Novel Transport Properties of Colonic Crypt Cells: Fluid Absorption and Cl-Dependent Na-H Exchange
Binder H, Singh S, Geibel J, Rajendran V. Novel Transport Properties of Colonic Crypt Cells: Fluid Absorption and Cl-Dependent Na-H Exchange. Comparative Biochemistry And Physiology Part A Molecular & Integrative Physiology 1997, 118: 265-269. PMID: 9366054, DOI: 10.1016/s0300-9629(97)00079-0.Peer-Reviewed Original ResearchConceptsNa-H exchangeCrypt cellsFluid absorptionLamina propria cellsColonic ion transportNet fluid secretionRat distal colonColonic crypt cellsPresence of myofibroblastsApical membrane vesiclesSecretory processRinger-like solutionDibutyryl cyclic AMPDistal colonCrypt preparationNeurohumoral agonistsAcid loadMorphologic techniquesFluid secretionGradient stimulationAbsorptive processesCyclic AMPIndividual cryptsMyofibroblastsBath solution
1994
Apical membrane Cl-butyrate exchange: Mechanism of short chain fatty acid stimulation of active chloride absorption in rat distal colon
Rajendran V, Binder H. Apical membrane Cl-butyrate exchange: Mechanism of short chain fatty acid stimulation of active chloride absorption in rat distal colon. The Journal Of Membrane Biology 1994, 141: 51-58. PMID: 7966245, DOI: 10.1007/bf00232873.Peer-Reviewed Original ResearchConceptsRat distal colonFatty acid stimulationDistal colonShort-chain fatty acidsNa-H exchangeActive chloride absorptionHalf maximal inhibitory concentrationAcid stimulationApical membrane vesiclesChain fatty acidsChloride absorptionFatty acid exchangeCl- gradientInhibitory concentrationOutward Cl gradientButyrate concentrationExtravesicular ClColonCellular modelMembrane vesiclesFatty acidsStimulationFunctional NaDisulfonic acidCompelling evidence