2009
W1646 DSS-Induced Colonic Inflammation Inhibits HCO3-Stimulated But Not Butyrate-Stimulated Na Absorption in Rat Distal Colon
Shanmugam N, Kashgarian M, Binder H, Rajendran V. W1646 DSS-Induced Colonic Inflammation Inhibits HCO3-Stimulated But Not Butyrate-Stimulated Na Absorption in Rat Distal Colon. Gastroenterology 2009, 136: a-709. DOI: 10.1016/s0016-5085(09)63270-4.Peer-Reviewed Original Research
2004
Three distinct mechanisms of HCO3− secretion in rat distal colon
Vidyasagar S, Rajendran VM, Binder HJ. Three distinct mechanisms of HCO3− secretion in rat distal colon. American Journal Of Physiology - Cell Physiology 2004, 287: c612-c621. PMID: 15308466, DOI: 10.1152/ajpcell.00474.2003.Peer-Reviewed Original ResearchConceptsRat distal colonShort-chain fatty acidsColonic mucosaDistal colonRat distal colonic mucosaDistal colonic mucosaCl- channel blockerSCFA/Channel blockersSerosal layerCrypt cellsCFTR blockerMicroM DIDSApical membrane anion channelApical membraneSecretionDependent secretionColonLumen ClSecretory mechanismMammalian colonBlockersMucosaStat techniqueDIDS
2003
Apical NHE isoforms differentially regulate butyrate-stimulated Na absorption in rat distal colon
Krishnan S, Rajendran VM, Binder HJ. Apical NHE isoforms differentially regulate butyrate-stimulated Na absorption in rat distal colon. American Journal Of Physiology - Cell Physiology 2003, 285: c1246-c1254. PMID: 12878493, DOI: 10.1152/ajpcell.00598.2002.Peer-Reviewed Original ResearchBumetanide-sensitive transporter mediates HCO3− uptake across basolateral membranes in rat distal colon
Vidyasagar S, Rajendran V, Binder H. Bumetanide-sensitive transporter mediates HCO3− uptake across basolateral membranes in rat distal colon. Gastroenterology 2003, 124: a311. DOI: 10.1016/s0016-5085(03)81566-4.Peer-Reviewed Original ResearchBumetanide-sensitive transporterRat distal colon
2002
HKcαEncodes Ouabain-insensitive H,K-ATPase in Surface Cells of Rat Distal Colon
Binder H. HKcαEncodes Ouabain-insensitive H,K-ATPase in Surface Cells of Rat Distal Colon. 2002, 43-52. DOI: 10.1007/978-1-4615-0971-4_4.Peer-Reviewed Original ResearchRenal functionK secretionActive K secretionNormal renal functionReduced renal functionActive K absorptionDietary K intakeNormal dietary intakeRat distal colonNet K secretionMammalian colonK-ATPaseK homeostasisActive transport mechanismK intakeDistal colonDietary intakeExperimental animalsLarge intestineK absorptionTransport mechanismColonSecretionImportant siteNormal conditions
2001
Characterization of apical membrane Cl-dependent Na/H exchange in crypt cells of rat distal colon
Rajendran V, Geibel J, Binder H. Characterization of apical membrane Cl-dependent Na/H exchange in crypt cells of rat distal colon. AJP Gastrointestinal And Liver Physiology 2001, 280: g400-g405. PMID: 11171622, DOI: 10.1152/ajpgi.2001.280.3.g400.Peer-Reviewed Original ResearchConceptsRat distal colonNa/H exchangeCl- channel blockerDistal colonChannel blockersCrypt cellsIntracellular pH recoveryExchange inhibitor amilorideColonic epithelial cellsApical membrane vesiclesCrypt glandsHigh doseHOE 694NA depletionAcid loadExchange inhibitorInhibitor amiloridePH recoveryEpithelial cellsUptake 1NHE isoformsAnion exchange inhibitorBlockersColonNa uptake
2000
Cl‐Dependent Na‐H Exchange: A Novel Colonic Crypt Transport Mechanism
BINDER H, RAJENDRAN V, GEIBEL J. Cl‐Dependent Na‐H Exchange: A Novel Colonic Crypt Transport Mechanism. Annals Of The New York Academy Of Sciences 2000, 915: 43-53. PMID: 11193600, DOI: 10.1111/j.1749-6632.2000.tb05222.x.Peer-Reviewed Original ResearchBasolateral K-Cl Cotransporter Regulates Colonic Potassium Absorption in Potassium Depletion*
Sangan P, Brill S, Sangan S, Forbush B, Binder H. Basolateral K-Cl Cotransporter Regulates Colonic Potassium Absorption in Potassium Depletion*. Journal Of Biological Chemistry 2000, 275: 30813-30816. PMID: 10878016, DOI: 10.1074/jbc.m003931200.Peer-Reviewed Original ResearchConceptsK-Cl cotransporterMembrane HK-Cl cotransportActive potassium absorptionProtein expressionBasolateral membranePotassium absorptionK-ATPaseNorthern blot analysisKCC1 mRNACoordinated regulationAlpha-subunit mRNABeta-subunit mRNAMRNA abundanceBlot analysisMRNAExpressionProteinMembraneCotransporterDepletionRat distal colonCDNAKCC1Colonic H-K-ATPase α- and β-subunits express ouabain-insensitive H-K-ATPase
Sangan P, Thevananther S, Sangan S, Rajendran V, Binder H. Colonic H-K-ATPase α- and β-subunits express ouabain-insensitive H-K-ATPase. American Journal Of Physiology - Cell Physiology 2000, 278: c182-c189. PMID: 10644526, DOI: 10.1152/ajpcell.2000.278.1.c182.Peer-Reviewed Original Research
1999
Stimulation of Sodium Chloride Absorption from Secreting Rat Colon by Short-Chain Fatty Acids
Krishnan S, Ramakrishna BS, Binder H. Stimulation of Sodium Chloride Absorption from Secreting Rat Colon by Short-Chain Fatty Acids. Digestive Diseases And Sciences 1999, 44: 1924-1930. PMID: 10505736, DOI: 10.1023/a:1018871412748.Peer-Reviewed Original ResearchConceptsShort-chain fatty acidsNa absorptionNaCl absorptionControl normal mucosaNet Cl secretionRat distal colonSodium chloride absorptionPresence of SCFAEffect of butyrateElectroneutral NaCl absorptionVoltage-clamp conditionsFatty acidsDistal colonNormal mucosaRat colonAbsorptive pathwaysCl(-) secretionChloride absorptionColonCAMP elevationSCFAMucosaSecretagoguesAdenosine monophosphateSecretionColonic H-K-ATPase β-subunit: identification in apical membranes and regulation by dietary K depletion
Sangan P, Kolla S, Rajendran V, Kashgarian M, Binder H. Colonic H-K-ATPase β-subunit: identification in apical membranes and regulation by dietary K depletion. American Journal Of Physiology 1999, 276: c350-c360. PMID: 9950762, DOI: 10.1152/ajpcell.1999.276.2.c350.Peer-Reviewed Original ResearchRole of Cl channels in Cl-dependent Na/H exchange
Rajendran V, Geibel J, Binder H. Role of Cl channels in Cl-dependent Na/H exchange. American Journal Of Physiology 1999, 276: g73-g78. PMID: 9886981, DOI: 10.1152/ajpgi.1999.276.1.g73.Peer-Reviewed Original ResearchConceptsNa/H exchangeCl- channel blockerChannel blockersMicroM DIDSNa/H exchange activityCystic fibrosis transmembrane conductance regulatorIntracellular pH recoveryRat distal colonCl- channelsH exchange activityDistal colonCrypt lumenCrypt cellsAcid loadExchange inhibitorApical membranePH recoveryCl(-) dependenceAnion exchange inhibitorCl- channel activityChannel activityBlockersH exchangePolyclonal antibodiesCl- conductanceDistribution and regulation of apical Cl/anion exchanges in surface and crypt cells of rat distal colon
Rajendran V, Binder H. Distribution and regulation of apical Cl/anion exchanges in surface and crypt cells of rat distal colon. American Journal Of Physiology 1999, 276: g132-g137. PMID: 9886988, DOI: 10.1152/ajpgi.1999.276.1.g132.Peer-Reviewed Original Research
1998
Role of Apical H-K Exchange and Basolateral K Channel in the Regulation of Intracellular pH in Rat Distal Colon Crypt Cells
Ikuma M, Binder H, Geibel J. Role of Apical H-K Exchange and Basolateral K Channel in the Regulation of Intracellular pH in Rat Distal Colon Crypt Cells. The Journal Of Membrane Biology 1998, 166: 205-212. PMID: 9843594, DOI: 10.1007/s002329900462.Peer-Reviewed Original ResearchMeSH KeywordsAnimalsAntiportersBariumCell PolarityColonEpithelial CellsH(+)-K(+)-Exchanging ATPaseHomeostasisHydrogen-Ion ConcentrationIntestinal MucosaIntracellular FluidIon TransportMaleOuabainPotassiumPotassium ChannelsPotassium-Hydrogen AntiportersRatsRats, Sprague-DawleySodium-Potassium-Exchanging ATPaseCl−HCO3 and Cl−OH exchanges of basolateral membranes (BLM) regulate intracellular pH (pHi) in crypt cells of rat distal colon
Ikuma M, Geibel J, Binder H, Rajendran V. Cl−HCO3 and Cl−OH exchanges of basolateral membranes (BLM) regulate intracellular pH (pHi) in crypt cells of rat distal colon. Gastroenterology 1998, 114: a380. DOI: 10.1016/s0016-5085(98)81539-4.Peer-Reviewed Original ResearchCrosstalk between apical H, K-ATPase and basolateral K channel regulates intracellular (pHi) in rat distal colon
Ikuma M, Binder H, Geibel J. Crosstalk between apical H, K-ATPase and basolateral K channel regulates intracellular (pHi) in rat distal colon. Gastroenterology 1998, 114: a380. DOI: 10.1016/s0016-5085(98)81540-0.Peer-Reviewed Original ResearchRat distal colonBasolateral K channelsDifferential localization of colonic H+-K+-ATPase isoforms in surface and crypt cells
Rajendran V, Singh S, Geibel J, Binder H. Differential localization of colonic H+-K+-ATPase isoforms in surface and crypt cells. American Journal Of Physiology 1998, 274: g424-g429. PMID: 9486199, DOI: 10.1152/ajpgi.1998.274.2.g424.Peer-Reviewed Original ResearchConceptsCrypt cellsColonic cryptsRat distal colonSurface cellsSurface epithelial cellsATPase isoformsOuabain-insensitive componentApical membraneDistal colonAcid loadDependent intracellularEpithelial cellsATPase activityCryptsDifferential localizationOuabainATPase alpha subunitCellsAlpha subunitCompelling evidenceIsoformsColonActivityRecovery
1997
Novel Transport Properties of Colonic Crypt Cells: Fluid Absorption and Cl-Dependent Na-H Exchange
Binder H, Singh S, Geibel J, Rajendran V. Novel Transport Properties of Colonic Crypt Cells: Fluid Absorption and Cl-Dependent Na-H Exchange. Comparative Biochemistry And Physiology Part A Molecular & Integrative Physiology 1997, 118: 265-269. PMID: 9366054, DOI: 10.1016/s0300-9629(97)00079-0.Peer-Reviewed Original ResearchConceptsNa-H exchangeCrypt cellsFluid absorptionLamina propria cellsColonic ion transportNet fluid secretionRat distal colonColonic crypt cellsPresence of myofibroblastsApical membrane vesiclesSecretory processRinger-like solutionDibutyryl cyclic AMPDistal colonCrypt preparationNeurohumoral agonistsAcid loadMorphologic techniquesFluid secretionGradient stimulationAbsorptive processesCyclic AMPIndividual cryptsMyofibroblastsBath solutionRegulation of colonic H-K-ATPase in large intestine and kidney by dietary Na depletion and dietary K depletion
Sangan P, Rajendran V, Mann A, Kashgarian M, Binder H. Regulation of colonic H-K-ATPase in large intestine and kidney by dietary Na depletion and dietary K depletion. American Journal Of Physiology 1997, 272: c685-c696. PMID: 9124313, DOI: 10.1152/ajpcell.1997.272.2.c685.Peer-Reviewed Original ResearchConceptsDietary K depletionDistal colonNA depletionK-ATPase activityProximal colonLarge intestineRat distal colonActive K absorptionK-ATPaseRenal collecting ductsDietary NaK-ATPase expressionK-ATPase isoformsK depletionRat colonCollecting ductsOuter medullaColonProtein expressionProtein levelsKidneyPolyclonal antibodiesIntestineDuctMRNA
1995
Fluid absorption in isolated perfused colonic crypts.
Singh S, Binder H, Boron W, Geibel J. Fluid absorption in isolated perfused colonic crypts. Journal Of Clinical Investigation 1995, 96: 2373-2379. PMID: 7593625, PMCID: PMC185889, DOI: 10.1172/jci118294.Peer-Reviewed Original ResearchConceptsCrypt cellsNM vasoactive intestinal peptideFluid absorptionLarge intestinal functionColonic cryptsVasoactive intestinal peptideLamina propria cellsNet fluid secretionRat distal colonNet fluid absorptionDibutyryl cyclic AMPIntestinal peptideDistal colonIntestinal functionLamina propriaPathophysiological paradigmCrypt preparationMM acetylcholineBasal stateNeurohumoral agonistsFluid secretionCellular elementsEpithelial cellsIntestinal cryptsCrypts