2013
Identification of Functionally Distinct Na-HCO3 Co-Transporters in Colon
Barmeyer C, Ye JH, Soroka C, Geibel P, Hingsammer LM, Weitgasser L, Atway D, Geibel JP, Binder HJ, Rajendran VM. Identification of Functionally Distinct Na-HCO3 Co-Transporters in Colon. PLOS ONE 2013, 8: e62864. PMID: 23690961, PMCID: PMC3653958, DOI: 10.1371/journal.pone.0062864.Peer-Reviewed Original ResearchConceptsNa-HCO3 cotransportDistal colonProximal colonNBC activityNBC isoformsRat colonColonic epithelial cellsCrypt glandsSensitive transporterHCO3- secretionColonHCO3-dependent mechanismEpithelial cellsSaturable processHOE694Isoform 1Basolateral membraneCotransportPCR analysisNorthern blotDIDSMolecular studiesMolecular identityApparent KiCritical role
2000
Colonic H-K-ATPase α- and β-subunits express ouabain-insensitive H-K-ATPase
Sangan P, Thevananther S, Sangan S, Rajendran V, Binder H. Colonic H-K-ATPase α- and β-subunits express ouabain-insensitive H-K-ATPase. American Journal Of Physiology - Cell Physiology 2000, 278: c182-c189. PMID: 10644526, DOI: 10.1152/ajpcell.2000.278.1.c182.Peer-Reviewed Original Research
1999
Stimulation of Sodium Chloride Absorption from Secreting Rat Colon by Short-Chain Fatty Acids
Krishnan S, Ramakrishna BS, Binder H. Stimulation of Sodium Chloride Absorption from Secreting Rat Colon by Short-Chain Fatty Acids. Digestive Diseases And Sciences 1999, 44: 1924-1930. PMID: 10505736, DOI: 10.1023/a:1018871412748.Peer-Reviewed Original ResearchConceptsShort-chain fatty acidsNa absorptionNaCl absorptionControl normal mucosaNet Cl secretionRat distal colonSodium chloride absorptionPresence of SCFAEffect of butyrateElectroneutral NaCl absorptionVoltage-clamp conditionsFatty acidsDistal colonNormal mucosaRat colonAbsorptive pathwaysCl(-) secretionChloride absorptionColonCAMP elevationSCFAMucosaSecretagoguesAdenosine monophosphateSecretionDifferential regulation of NHE isoforms by sodium depletion in proximal and distal segments of rat colon
Ikuma M, Kashgarian M, Binder H, Rajendran V. Differential regulation of NHE isoforms by sodium depletion in proximal and distal segments of rat colon. American Journal Of Physiology 1999, 276: g539-g549. PMID: 9950829, DOI: 10.1152/ajpgi.1999.276.2.g539.Peer-Reviewed Original ResearchConceptsDietary sodium depletionSodium depletionDistal colonRat colonProtein expressionMRNA abundanceBlot analysisDose-dependent inhibitorApical membrane vesiclesElectroneutral NaCl absorptionWestern blot analysisDifferential regulationNormal ratsProximal colonHOE 694Multiple diverse effectsColonDistal segmentsNaCl absorptionNHE isoformsExchange activityExchanger isoformsNorthern blot analysisDifferential effectsPretranslational level
1997
Regulation of colonic H-K-ATPase in large intestine and kidney by dietary Na depletion and dietary K depletion
Sangan P, Rajendran V, Mann A, Kashgarian M, Binder H. Regulation of colonic H-K-ATPase in large intestine and kidney by dietary Na depletion and dietary K depletion. American Journal Of Physiology 1997, 272: c685-c696. PMID: 9124313, DOI: 10.1152/ajpcell.1997.272.2.c685.Peer-Reviewed Original ResearchConceptsDietary K depletionDistal colonNA depletionK-ATPase activityProximal colonLarge intestineRat distal colonActive K absorptionK-ATPaseRenal collecting ductsDietary NaK-ATPase expressionK-ATPase isoformsK depletionRat colonCollecting ductsOuter medullaColonProtein expressionProtein levelsKidneyPolyclonal antibodiesIntestineDuctMRNA
1993
Ion Transport in Rat Colon
Rajendran V, Binder H. Ion Transport in Rat Colon. Advances In Comparative And Environmental Physiology 1993, 16: 113-137. DOI: 10.1007/978-3-642-77118-7_6.Peer-Reviewed Original Research
1986
Demonstration of Specific High Affinity Receptors for Aldosterone in Cytosol of Rat Colon*
BINDER H, WHITE A, WHITING D, HAYSLETT J. Demonstration of Specific High Affinity Receptors for Aldosterone in Cytosol of Rat Colon*. Endocrinology 1986, 118: 628-631. PMID: 2935388, DOI: 10.1210/endo-118-2-628.Peer-Reviewed Original ResearchConceptsRat distal colonSpecific high-affinity receptorsDistal colonHigh-affinity receptorFmol/Affinity receptorAction of aldosteroneGlucocorticoid receptor systemHigh-affinity binding proteinsRU 28362Competitive binding studiesScatchard plot analysisAldosteroneHigh affinity receptor proteinAldosterone bindingAffinity sitesRat colonGlucocorticoid receptorSpecific high affinity receptor proteinColonReceptor systemDirect actionCytosolic receptorReceptorsLow affinity sitesIon transport in proximal colon of the rat. Sodium depletion stimulates neutral sodium chloride absorption.
Foster E, Budinger M, Hayslett J, Binder H. Ion transport in proximal colon of the rat. Sodium depletion stimulates neutral sodium chloride absorption. Journal Of Clinical Investigation 1986, 77: 228-235. PMID: 2418060, PMCID: PMC423331, DOI: 10.1172/jci112281.Peer-Reviewed Original ResearchMeSH Keywords4-Acetamido-4'-isothiocyanatostilbene-2,2'-disulfonic Acid4,4'-Diisothiocyanostilbene-2,2'-Disulfonic AcidAcetazolamideAmilorideAnimalsBiological Transport, ActiveColonHyperaldosteronismIntestinal AbsorptionIntestinal MucosaIon ChannelsMaleRatsRats, Inbred StrainsSodium ChlorideTheophyllineConceptsMu eq/hSodium-hydrogen exchangeProximal colonSodium depletionSodium absorptionDistal colonNeutral sodium chloride absorptionDistal segmentsAmiloride-sensitive sodium absorptionNet sodium absorptionSodium-depleted ratsRat distal colonSodium chloride absorptionNeutral sodium absorptionHydrogen ion secretionSecondary hyperaldosteronismParallel fallRat colonControl groupMM amilorideMucosal amilorideJNanetColonIon secretionChloride absorption
1983
Role of calcium in the regulation of colonic secretion in the rat
Zimmerman T, Dobbins J, Binder H. Role of calcium in the regulation of colonic secretion in the rat. American Journal Of Physiology 1983, 244: g552-g560. PMID: 6133455, DOI: 10.1152/ajpgi.1983.244.5.g552.Peer-Reviewed Original ResearchConceptsSerosal calciumRole of calciumExtracellular calciumElectrolyte transportNeutral sodium chloride absorptionEffect of bethanecholEffect of theophyllineMuscarinic cholinergic agonistNet sodium absorptionSodium chloride absorptionNet chloride absorptionCalcium-free mediumNeutral NaCl absorptionColonic secretionCholinergic agonistsIntracellular calciumBethanecholNet sodiumRat colonChloride secretionJNanetSodium absorptionChloride absorptionJClnetNaCl absorptionCyclic Adenosine Monophosphate Stimulates Active Potassium Secretion in the Rat Colon
Foster E, Sandle G, Hayslett J, Binder H. Cyclic Adenosine Monophosphate Stimulates Active Potassium Secretion in the Rat Colon. Gastroenterology 1983, 84: 324-330. PMID: 6293907, DOI: 10.1016/s0016-5085(83)80130-9.Peer-Reviewed Original ResearchConceptsActive potassium secretionNormal potassium dietPotassium secretionNet potassium secretionPotassium dietCyclic adenosine monophosphateDibutyryl cyclic adenosine monophosphateRat colonAdenosine monophosphatePotassium-loaded animalsCholinergic muscarinic agonistHigh potassium dietProximal colonic mucosaCalcium-dependent processesX cm2
1981
THE EFFECT OF SOMATOSTATIN AND ENKEPHALIN ON ION TRANSPORT IN THE INTESTINE*
Dobbins J, Dharmsathaphorn K, Racusen L, Binder H. THE EFFECT OF SOMATOSTATIN AND ENKEPHALIN ON ION TRANSPORT IN THE INTESTINE*. Annals Of The New York Academy Of Sciences 1981, 372: 594-612. PMID: 6122417, DOI: 10.1111/j.1749-6632.1981.tb15508.x.Peer-Reviewed Original ResearchConceptsEffect of somatostatinFinal common pathwayCa channel blockersCa-free solutionCyclic AMP levelsInflux of NaCarcinoid syndromeIntestinal motilityAntidiarrheal effectSRIF infusionSecretory diarrheaRat colonRabbit ileumAMP levelsElectrolyte absorptionStimulatory effectC1 absorptionEnkephalinDistal stepBrush border membraneCommon pathwayDiarrheaCyclic AMPNet NaSRIF