2013
Identification of Functionally Distinct Na-HCO3 Co-Transporters in Colon
Barmeyer C, Ye JH, Soroka C, Geibel P, Hingsammer LM, Weitgasser L, Atway D, Geibel JP, Binder HJ, Rajendran VM. Identification of Functionally Distinct Na-HCO3 Co-Transporters in Colon. PLOS ONE 2013, 8: e62864. PMID: 23690961, PMCID: PMC3653958, DOI: 10.1371/journal.pone.0062864.Peer-Reviewed Original ResearchConceptsNa-HCO3 cotransportDistal colonProximal colonNBC activityNBC isoformsRat colonColonic epithelial cellsCrypt glandsSensitive transporterHCO3- secretionColonHCO3-dependent mechanismEpithelial cellsSaturable processHOE694Isoform 1Basolateral membraneCotransportPCR analysisNorthern blotDIDSMolecular studiesMolecular identityApparent KiCritical role
1994
Differential modulation of Na-HCO3 cotransport and Na-H exchange by pH in basolateral membrane vesicles of rat distal colon.
Rajendran V, Binder H. Differential modulation of Na-HCO3 cotransport and Na-H exchange by pH in basolateral membrane vesicles of rat distal colon. Journal Of Biological Chemistry 1994, 269: 156-160. PMID: 8276790, DOI: 10.1016/s0021-9258(17)42328-3.Peer-Reviewed Original ResearchConceptsMembrane vesiclesDifferent cellular functionsBasolateral membrane vesiclesProton concentration gradientCellular functionsIntravesicular protonsApparent KmVesiclesTransport systemNa-H exchangeCotransportDifferential modulationHigh proton concentrationProton concentrationProton stimulationNa-HCO3 cotransportConcentration gradientKmRat distal colonProteinSeparate mechanismsVmax
1987
Mechanism of electroneutral sodium chloride absorption in distal colon of the rat
Binder H, Foster E, Budinger M, Hayslett J. Mechanism of electroneutral sodium chloride absorption in distal colon of the rat. Gastroenterology 1987, 93: 449-455. PMID: 3609655, DOI: 10.1016/0016-5085(87)90905-x.Peer-Reviewed Original ResearchConceptsSodium chloride absorptionElectroneutral sodium chloride absorptionNet sodium absorptionNet chloride absorptionDistal colonChloride absorptionSodium absorptionRat distal colonSodium chloride cotransportVoltage-clamp conditionsMM furosemideMM amilorideMucosal bathing solutionChloride transportClamp conditionsColonBathing solutionRatsElectrogenic sodiumMechanism of sodiumAmilorideSodiumAbsence of potassiumCotransportMucosa