2023
HOXC6 drives a therapeutically targetable pancreatic cancer growth and metastasis pathway by regulating MSK1 and PPP2R2B
Malvi P, Chava S, Cai G, Hu K, Zhu L, Edwards Y, Green M, Gupta R, Wajapeyee N. HOXC6 drives a therapeutically targetable pancreatic cancer growth and metastasis pathway by regulating MSK1 and PPP2R2B. Cell Reports Medicine 2023, 4: 101285. PMID: 37951219, PMCID: PMC10694669, DOI: 10.1016/j.xcrm.2023.101285.Peer-Reviewed Original ResearchConceptsPancreatic ductal adenocarcinomaHomeobox C6PDAC growthInsulin-like growth factor 1 receptorGrowth factor 1 receptorKinase MSK1Factor 1 receptorTranscription factorsPancreatic cancer growthMSK1Tumor growthPDAC tumor growthMost pancreatic ductal adenocarcinomasMammalian targetIGF1R inhibitorsTherapeutic vulnerabilitiesRapamycin (mTOR) pathway activationMEK inhibitor trametinibMetastasis pathwaysPDAC mouse modelPDAC cellsMTOR inhibitionPharmacological inhibitionPathway activationInhibition blocksCytomorphologic, immunophenotypical and molecular features of pancreatic acinar cell carcinoma
Sun T, Gilani S, Jain D, Cai G. Cytomorphologic, immunophenotypical and molecular features of pancreatic acinar cell carcinoma. Diagnostic Cytopathology 2023, 51: 674-683. PMID: 37469257, DOI: 10.1002/dc.25196.Peer-Reviewed Original ResearchMeSH KeywordsCarcinoma, Acinar CellCarcinoma, Pancreatic DuctalHumansPancreasPancreatic NeoplasmsRepressor ProteinsConceptsPancreatic acinar cell carcinomaPancreatic ductal carcinomaAcinar cell carcinomaCell carcinomaMixed acinar-ductal carcinomaMixed acinar-neuroendocrine carcinomaMolecular featuresComprehensive molecular profilingDifferent molecular alterationsDistinct molecular featuresImmunophenotypical featuresNeuroendocrine featuresDuctal carcinomaRare tumorCCDC6-RETCytohistological correlationKi-67Large cohortCytologic diagnosisBiomarker assessmentCytology featuresCytology specimensCarcinomaMolecular alterationsGermline mutations
2022
Intraductal papillary neoplasm of the bile duct: Cytomorphologic and molecular features
Stendahl K, Gilani SM, Basturk O, Hui P, Sigel C, Cai G. Intraductal papillary neoplasm of the bile duct: Cytomorphologic and molecular features. Cancer Cytopathology 2022, 131: 37-49. PMID: 36041224, PMCID: PMC9812877, DOI: 10.1002/cncy.22637.Peer-Reviewed Original ResearchMeSH KeywordsAdenocarcinomaBile Duct NeoplasmsBile DuctsChromatinHumansPancreatic NeoplasmsProto-Oncogene Proteins p21(ras)Retrospective StudiesConceptsIntraductal papillary neoplasmBile ductCytomorphologic featuresFine granular chromatinPapillary neoplasmVacuolated cytoplasmGranular chromatinCase of IPNBColumnar cellsComplex papillary architectureOriginal cytological diagnosisCommon bile ductCharacteristic cytomorphologic featuresHigh-grade dysplasiaMolecular featuresBackground mucinKRAS testingHepatic ductRetrospective studyAcute inflammationHistopathologic confirmationKRAS mutationsPapillary architecturePapillary clustersCytological diagnosisComprehensive molecular profiling of pancreatic ductal adenocarcinoma in FNA, biopsy, and resection specimens
Razzano D, Bouza SJ, Hernandez PV, Wang M, Robert ME, Walther Z, Cai G. Comprehensive molecular profiling of pancreatic ductal adenocarcinoma in FNA, biopsy, and resection specimens. Cancer Cytopathology 2022, 130: 726-734. PMID: 35511415, DOI: 10.1002/cncy.22589.Peer-Reviewed Original ResearchMeSH KeywordsCarcinoma, Pancreatic DuctalEndoscopic Ultrasound-Guided Fine Needle AspirationHumansPancreatic NeoplasmsProto-Oncogene Proteins p21(ras)ConceptsFine-needle aspirationFine-needle biopsyPancreatic ductal adenocarcinomaOncomine Comprehensive AssayResection specimensMolecular alterationsMolecular testingFNB samplesDuctal adenocarcinomaTherapeutic implicationsSuccess rateComprehensive molecular analysisComprehensive molecular testingComprehensive molecular profilingPotential therapeutic implicationsSimilar success ratesResection casesKRAS mutationsFNB specimensFNA materialFNA specimensAmplification analysisMolecular profilingFusion assessmentGene mutationsSignificance of KRAS mutation testing in biliary brushing cytology specimens: A 10‐year retrospective review
Sun T, Zuo T, Hui P, Cai G. Significance of KRAS mutation testing in biliary brushing cytology specimens: A 10‐year retrospective review. Cancer Cytopathology 2022, 130: 558-565. PMID: 35417072, DOI: 10.1002/cncy.22579.Peer-Reviewed Original ResearchConceptsExtrahepatic biliary stenosisKRAS mutation testingKRAS mutational analysisKRAS mutationsBiliary stenosisBiliary ductsInstitutional review board approvalResults of cytologyPancreatic duct adenocarcinomaBiliary brushing cytologyReview board approvalCytological diagnostic categoriesRetrospective reviewBiliary stricturesDuct adenocarcinomaAbsolute riskCytology examinationDuct brushingsBiliary brushingsBrushing cytologyMalignant conditionsCytological diagnosisBoard approvalClinical practiceCytology specimens
2020
Endoscopic Ultrasound-Guided Fine Needle Aspiration Cytologic Evaluation of Intraductal Papillary Mucinous Neoplasm and Mucinous Cystic Neoplasms of Pancreas
Gilani SM, Adeniran AJ, Cai G. Endoscopic Ultrasound-Guided Fine Needle Aspiration Cytologic Evaluation of Intraductal Papillary Mucinous Neoplasm and Mucinous Cystic Neoplasms of Pancreas. American Journal Of Clinical Pathology 2020, 154: 559-570. PMID: 32589187, DOI: 10.1093/ajcp/aqaa079.Peer-Reviewed Original ResearchConceptsIntraductal papillary mucinous neoplasmMucinous cystic neoplasmMucinous cystic lesionsHigh-grade dysplasiaPapillary mucinous neoplasmCystic neoplasmsMucinous neoplasmsAncillary testingFine-needle aspiration cytologic evaluationUltrasound-guided fine-needle aspiration cytologyEndoscopic ultrasound-guided fine-needle aspiration cytologyFine needle aspiration cytologyRisk of malignancyNeedle aspiration cytologyUse of cytologyPositive cytologic diagnosisCytologic categoriesModerate dysplasiaCystic lesionsCytopathology guidelinesInvasive malignancyMalignancy rateHistologic diagnosisCytologic evaluationEndoscopic ultrasoundKLF7 promotes pancreatic cancer growth and metastasis by up-regulating ISG expression and maintaining Golgi complex integrity
Gupta R, Malvi P, Parajuli KR, Janostiak R, Bugide S, Cai G, Zhu LJ, Green MR, Wajapeyee N. KLF7 promotes pancreatic cancer growth and metastasis by up-regulating ISG expression and maintaining Golgi complex integrity. Proceedings Of The National Academy Of Sciences Of The United States Of America 2020, 117: 12341-12351. PMID: 32430335, PMCID: PMC7275752, DOI: 10.1073/pnas.2005156117.Peer-Reviewed Original ResearchConceptsGolgi complex integrityGolgi complex fragmentationKrüppel-like factor 7Complex integrityPDAC growthMAP kinase pathwayTumor suppressor p53Protein glycosylationPancreatic ductal adenocarcinomaKinase pathwayPancreatic cancer growthSuppressor p53PDAC tumor growthTumor growthISG expressionProtein 3Factor 7Cancer growthKLF7 expressionComplex fragmentationKnockdownPharmacologic activationGrowth factorCell culturesExpression
2019
Grading Pancreatic Neuroendocrine Tumors by Ki-67 Index Evaluated on Fine-Needle Aspiration Cell Block Material
Abi-Raad R, Lavik JP, Barbieri AL, Zhang X, Adeniran AJ, Cai G. Grading Pancreatic Neuroendocrine Tumors by Ki-67 Index Evaluated on Fine-Needle Aspiration Cell Block Material. American Journal Of Clinical Pathology 2019, 153: 74-81. PMID: 31415691, DOI: 10.1093/ajcp/aqz110.Peer-Reviewed Original Research
2017
Paraoxonase 2 Facilitates Pancreatic Cancer Growth and Metastasis by Stimulating GLUT1-Mediated Glucose Transport
Nagarajan A, Dogra SK, Sun L, Gandotra N, Ho T, Cai G, Cline G, Kumar P, Cowles RA, Wajapeyee N. Paraoxonase 2 Facilitates Pancreatic Cancer Growth and Metastasis by Stimulating GLUT1-Mediated Glucose Transport. Molecular Cell 2017, 67: 685-701.e6. PMID: 28803777, PMCID: PMC5567863, DOI: 10.1016/j.molcel.2017.07.014.Peer-Reviewed Original ResearchMeSH KeywordsAMP-Activated Protein KinasesAnimalsAntineoplastic AgentsApoptosis Regulatory ProteinsAryldialkylphosphataseCarcinoma, Pancreatic DuctalCell Line, TumorCell MovementCell ProliferationEnergy MetabolismFemaleForkhead Box Protein O3Gene Expression Regulation, NeoplasticGlucoseGlucose Transporter Type 1HumansLiver NeoplasmsLung NeoplasmsMaleMice, NudeMutationPancreatic NeoplasmsProtein Kinase InhibitorsProto-Oncogene ProteinsProto-Oncogene Proteins p21(ras)RNA InterferenceSignal TransductionTime FactorsTranscription, GeneticTransfectionTumor BurdenTumor Suppressor Protein p53Xenograft Model Antitumor AssaysConceptsPDAC tumor growthGlucose transportCellular starvation responsesParaoxonase 2Glutamine metabolism pathwayNew metabolic regulatorPDAC tumor samplesShort hairpin RNATumor growthStarvation responseMetabolic genesTranscriptional targetsProtein kinaseTractable pathwayPancreatic cancer growthGenetic activationMetabolism pathwaysHairpin RNAMetabolic regulatorNew modulatorsHuman cancersPancreatic ductal adenocarcinomaMetabolic deregulationAMPKCancer growth
2012
Cytoplasmic vacuolization: An under‐recognized cytomorphologic feature in endocrine tumors of the pancreas
Levy GH, Finkelstein A, Harigopal M, Chhieng D, Cai G. Cytoplasmic vacuolization: An under‐recognized cytomorphologic feature in endocrine tumors of the pancreas. Diagnostic Cytopathology 2012, 41: 623-628. PMID: 22807461, DOI: 10.1002/dc.22893.Peer-Reviewed Original ResearchConceptsFine-needle aspirationPancreatic endocrine neoplasmsCytomorphologic featuresDiagnostic pitfallsEndoscopic ultrasound-guided fine-needle aspirationUltrasound-guided fine-needle aspirationFine cytoplasmic vacuolesClinicopathologic featuresDiagnostic challengeEndocrine tumorsEndocrine neoplasmsSmall biopsiesAccurate diagnosisImmunocytochemical studyCytoplasmic vacuolesDiagnostic value of K‐ras mutation analysis for pancreaticobiliary cytology specimens with indeterminate diagnosis
Cai G, Mahooti S, Lipata FM, Chhieng D, Hui P. Diagnostic value of K‐ras mutation analysis for pancreaticobiliary cytology specimens with indeterminate diagnosis. Cancer Cytopathology 2012, 120: 313-318. PMID: 22367918, DOI: 10.1002/cncy.21188.Peer-Reviewed Original Research