2023
1510-P: Lipid-Induced Renal Cortical Insulin Resistance Perturbs Gluconeogenic and Oxidative Metabolism via an sn-1,2-diacylglycerol-PKCe-Insulin Receptor Kinase Axis In Vivo
HUBBARD B, GASPAR R, ZHANG D, KAHN M, NASIRI A, SHULMAN G. 1510-P: Lipid-Induced Renal Cortical Insulin Resistance Perturbs Gluconeogenic and Oxidative Metabolism via an sn-1,2-diacylglycerol-PKCe-Insulin Receptor Kinase Axis In Vivo. Diabetes 2023, 72 DOI: 10.2337/db23-1510-p.Peer-Reviewed Original ResearchInsulin receptor kinasePyruvate carboxylaseHyperinsulinemic-euglycemic clampMitochondrial pyruvate oxidationInsulin resistanceOxidative metabolismMitochondrial pyruvate carboxylaseReceptor kinaseInhibitory phosphorylationAktS473 phosphorylationKinase axisChow fed miceImpairs insulinPyruvate oxidationKnockin micePhosphorylationKey targetFortress BiotechFed micePKCεDiacylglycerolRenal cortexHFDMetabolismBasal conditions
2021
335-OR: Lipid-Induced Insulin Resistance in the Renal Cortex Is Associated with Plasma Membrane Sn-1,2-diacylglycerol Accumulation and PKCe Translocation
HUBBARD B, GASPAR R, ZHANG D, KAHN M, NASIRI A, ZHANG X, CLINE G, SHULMAN G. 335-OR: Lipid-Induced Insulin Resistance in the Renal Cortex Is Associated with Plasma Membrane Sn-1,2-diacylglycerol Accumulation and PKCe Translocation. Diabetes 2021, 70 DOI: 10.2337/db21-335-or.Peer-Reviewed Original ResearchHigh-fat dietInsulin receptorInsulin resistanceLipid-Induced Insulin ResistanceRC miceProtein kinase CεRegular chowHFD miceRenal cortexCitrate synthase fluxHyperinsulinemic-euglycemic clamp conditionsAktS473 phosphorylationFatty acid fluxPyruvate oxidationPKCε translocationPyruvate dehydrogenase fluxPhosphorylationDiacylglycerol accumulationHFD feedingFat dietSpouse/partnerFold increaseSynthase fluxTranslocationIonis Pharmaceuticals