1994
Effect of buprenorphine on pancreatic enzyme synthesis and secretion in normal rats and rats with acute edematous pancreatitis
Ogden JM, Modlin IM, Gorelick FS, Marks IN. Effect of buprenorphine on pancreatic enzyme synthesis and secretion in normal rats and rats with acute edematous pancreatitis. Digestive Diseases And Sciences 1994, 39: 2407-2415. PMID: 7525167, DOI: 10.1007/bf02087658.Peer-Reviewed Original ResearchMeSH KeywordsAcute DiseaseAmylasesAnimalsBuprenorphineCeruletideChymotrypsinogenEdemaLipaseMalePancreasPancreatitisRatsRats, Inbred StrainsTrypsinogenConceptsAcute pancreatitisPancreatic enzyme secretionPancreatic enzyme synthesisAmylase secretionAmylase contentDevelopment of APEnzyme secretionSevere acute pancreatitisEffects of buprenorphineEffects of opiatesExperimental acute pancreatitisAcute edematous pancreatitisGroups of ratsSite of administrationNegative feedback inhibitionPancreatic edemaPancreatic stimulantsEdematous pancreatitisNormal ratsPancreatic massPancreatic secretionPancreatitisGroup IIControl groupGroup I
1991
Agonist-regulated phosphorylation of the pancreatic cholecystokinin receptor
Klueppelberg UG, Gates LK, Gorelick FS, Miller LJ. Agonist-regulated phosphorylation of the pancreatic cholecystokinin receptor. Journal Of Biological Chemistry 1991, 266: 2403-2408. PMID: 1989991, DOI: 10.1016/s0021-9258(18)52258-4.Peer-Reviewed Original ResearchMeSH KeywordsAdenosine TriphosphateAnimalsCarbacholCell FractionationChromatography, AffinityChromatography, High Pressure LiquidElectrophoresis, Polyacrylamide GelEnzyme ActivationKineticsMalePancreasPhosphorylationProtein Kinase CRatsRats, Inbred StrainsReceptors, CholecystokininSincalideSolubilityTetradecanoylphorbol AcetateConceptsC activationSodium dodecyl sulfate-polyacrylamide gelsDodecyl sulfate-polyacrylamide gelsProtein kinase C activationPlasma membrane glycoproteinsPhosphorylated MrKinase C activationSulfate-polyacrylamide gelsSerine residuesRegulated mannerAffinity-labeled proteinsMembrane glycoproteinsPhosphorylationReceptor proteinProteinNative formATP poolAffinity resinLectin affinity chromatographyConcentration-dependent mannerDirect activationPancreatic cholecystokinin receptorSubsequent solubilizationReceptorsActivationIntracellular activation of digestive zymogens in rat pancreatic acini. Stimulation by high doses of cholecystokinin.
Leach SD, Modlin IM, Scheele GA, Gorelick FS. Intracellular activation of digestive zymogens in rat pancreatic acini. Stimulation by high doses of cholecystokinin. Journal Of Clinical Investigation 1991, 87: 362-366. PMID: 1985109, PMCID: PMC295064, DOI: 10.1172/jci114995.Peer-Reviewed Original ResearchConceptsPancreatic aciniIntracellular activationCysteine protease inhibitor E64Digestive zymogensDose of cholecystokininRat pancreatic aciniProtease inhibitor E64Acute pancreatitisCholecystokininHigh dosesSerine protease activityAcidic subcellular compartmentsWeak base chloroquineImmunologic techniquesInhibitor E64Protonophore monensinPancreatitisIncubation mediumAciniBase chloroquineActivationIntracellular sitesProtease inhibitor benzamidineCell pelletsElectrophoretic mobility
1990
Regulated phosphorylation of secretory granule membrane proteins of the rat parotid gland
Marino CR, Castle JD, Gorelick FS. Regulated phosphorylation of secretory granule membrane proteins of the rat parotid gland. American Journal Of Physiology 1990, 259: g70-g77. PMID: 1695488, DOI: 10.1152/ajpgi.1990.259.1.g70.Peer-Reviewed Original ResearchConceptsSecretory granule membrane proteinsIntegral membrane proteinsMembrane proteinsGranule membrane proteinProtein phosphorylation eventsGranule membrane fractionExocrine secretory granulesPhosphorylation eventsSodium dodecyl sulfate-polyacrylamide gel electrophoresisDodecyl sulfate-polyacrylamide gel electrophoresisRat parotid glandRegulated phosphorylationSulfate-polyacrylamide gel electrophoresisSitu phosphorylationIntact cellsMembrane fractionTime-dependent mannerPhosphorylationProteinSecretory granule fractionParotid lobulesGel electrophoresisProtein antiserumSecretory granulesPhosphoprotein
1989
Improving the quality of immunoblots by chromatography of polyclonal antisera on keratin affinity columns
Girault J, Gorelick F, Greengard P. Improving the quality of immunoblots by chromatography of polyclonal antisera on keratin affinity columns. Analytical Biochemistry 1989, 182: 193-196. PMID: 2481415, DOI: 10.1016/0003-2697(89)90741-0.Peer-Reviewed Original ResearchPresence of calcium/calmodulin‐dependent protein Kinase II in Nerve terminals of rat brain
Walaas S, Gorelick F, Greengard P. Presence of calcium/calmodulin‐dependent protein Kinase II in Nerve terminals of rat brain. Synapse 1989, 3: 356-362. PMID: 2545012, DOI: 10.1002/syn.890030409.Peer-Reviewed Original ResearchMeSH KeywordsAnimalsCalcium-Calmodulin-Dependent Protein KinasesCorpus StriatumMaleNerve EndingsProtein KinasesRatsRats, Inbred StrainsSubstantia NigraConceptsCalcium/calmodulin-dependent protein kinase IICalmodulin-dependent protein kinase IISubstantia nigraProtein kinase IINerve terminalsRat brainSynapsin INerve terminal markersLesion-induced degenerationLesion-induced changesCalcium/calmodulin-dependent protein kinase type IIPrevious immunocytochemical studiesKinase IIStriatonigral tractCorticostriatal tractPresynaptic terminalsNeuronal populationsCalmodulin-dependent protein kinase type IIMammalian brainCell bodiesImmunocytochemical studyTerminal markersBrainNeostriatumLesions
1988
Cell-specific localization of the alpha-subunit of calcium/calmodulin-dependent protein kinase II in Purkinje cells in rodent cerebellum.
Walaas SI, Lai Y, Gorelick FS, DeCamilli P, Moretti M, Greengard P. Cell-specific localization of the alpha-subunit of calcium/calmodulin-dependent protein kinase II in Purkinje cells in rodent cerebellum. Brain Research 1988, 464: 233-42. PMID: 2850084, DOI: 10.1016/0169-328x(88)90029-0.Peer-Reviewed Original ResearchConceptsCalmodulin-dependent protein kinase type IICalcium/calmodulin-dependent protein kinase type IIProtein kinase type IIKinase type IICalmodulin-dependent protein kinase IICalcium/calmodulin-dependent protein kinase IIDistinct isozymic formsProtein kinase IINormal rat cerebellumPurkinje cellsBeta/betaNon-Purkinje cellsKinase IIAlpha subunitCell-specific localizationRestricted localizationRodent cerebellumIsozymic formsDifferent cellsImmunocytochemical analysisMutant miceSubunitsType IICellsCerebellum
1987
Calcium-calmodulin-stimulated protein kinase in developing pancreas
Gorelick FS, Chang A, Jamieson JD. Calcium-calmodulin-stimulated protein kinase in developing pancreas. American Journal Of Physiology 1987, 253: g469-g476. PMID: 3116856, DOI: 10.1152/ajpgi.1987.253.4.g469.Peer-Reviewed Original ResearchMeSH KeywordsAnimalsCalciumCalmodulinNerve Tissue ProteinsPancreasPeptide MappingProtein KinasesRatsRats, Inbred StrainsReference ValuesSynapsinsTrifluoperazineConceptsCalmodulin-dependent protein kinaseProtein kinase activityCDPK activityProtein kinaseKinase activityEndogenous proteinsType II calmodulin-dependent protein kinaseType II Ca2Adult pancreasCaM-binding proteinsRelative molecular weightEmbryonic pancreasPancreatic developmentPancreatic acinar cellsGel filtration chromatographySecretagogue responsivenessCalcium-calmodulinExogenous substratesKinaseSynapsin IPancreatic maturationProteinPancreatic supernatantPhosphorylationSecretory process