2002
Neutrophils and NADPH oxidase mediate intrapancreatic trypsin activation in murine experimental acute pancreatitis
Gukovskaya AS, Vaquero E, Zaninovic V, Gorelick FS, Lusis AJ, Brennan M, Holland S, Pandol SJ. Neutrophils and NADPH oxidase mediate intrapancreatic trypsin activation in murine experimental acute pancreatitis. Gastroenterology 2002, 122: 974-984. PMID: 11910350, DOI: 10.1053/gast.2002.32409.Peer-Reviewed Original ResearchConceptsIntrapancreatic trypsin activationAcute pancreatitisNADPH oxidaseTrypsin activationAdenine Dinucleotide Phosphate OxidaseParenchymal cell injuryExperimental acute pancreatitisNADPH oxidase activityPancreatic acinar tissueDigestive enzymesWestern blot analysisAntineutrophil serumPathologic activationCell injuryPancreatitisExperimental pancreatitisHigh dosesMarked attenuationNeutrophilsAbstractTextAcinar tissuePancreatic homogenatesReactive oxygen speciesMyeloperoxidaseMice
2001
Exiting the endoplasmic reticulum
Gorelick F, Shugrue C. Exiting the endoplasmic reticulum. Molecular And Cellular Endocrinology 2001, 177: 13-18. PMID: 11377815, DOI: 10.1016/s0303-7207(01)00438-5.Peer-Reviewed Original ResearchConceptsEndoplasmic reticulumNascent proteinsCOPII coatCoat protein complexCis-Golgi regionVesicular transport processesDifferent adaptor proteinsTrans-Golgi regionSite of synthesisCOPII vesiclesNascent vesiclesVesicle biogenesisCargo selectionCOPII proteinsExtracellular destinationsCOPI coatomerGolgi traffickingBudding vesicleCytoplasmic coatRetrograde trafficProtein complexesAdaptor proteinDonor membranesLysosome biogenesisTarget membrane
1999
Identification of the putative mammalian orthologue of Sec31P, a component of the COPII coat
Shugrue C, Kolen E, Peters H, Czernik A, Kaiser C, Matovcik L, Hubbard A, Gorelick F. Identification of the putative mammalian orthologue of Sec31P, a component of the COPII coat. Journal Of Cell Science 1999, 112: 4547-4556. PMID: 10574704, PMCID: PMC5567750, DOI: 10.1242/jcs.112.24.4547.Peer-Reviewed Original ResearchMeSH KeywordsAmino Acid SequenceAnimalsBase SequenceCarrier ProteinsCell LineCloning, MolecularCOP-Coated VesiclesDNA, ComplementaryFungal ProteinsGTPase-Activating ProteinsHumansMembrane ProteinsMolecular Sequence DataNuclear Pore Complex ProteinsPhosphoproteinsRatsSaccharomyces cerevisiaeSaccharomyces cerevisiae ProteinsSequence Homology, Amino AcidVesicular Transport ProteinsConceptsMammalian orthologuesCOPII coatSequential column chromatographyTwo-hybrid analysisIntracellular vesicular traffickingSmall punctate structuresVesicle-associated proteinLiver cDNA libraryCultured cell linesRat liver cDNA libraryVesicular buddingVesicular traffickingNovel proteinSignificant homologyTarget membraneCDNA libraryPunctate structuresSec13pSec31pIntact cellsP137ProteinOrthologuesCell linesColumn chromatography
1994
Antibodies to an epitope on synapsin I detect a protein associated with the endocytic compartment in non-neuronal cells.
Matovcik LM, Karapetian O, Czernik AJ, Marino CR, Kinder BK, Gorelick FS. Antibodies to an epitope on synapsin I detect a protein associated with the endocytic compartment in non-neuronal cells. European Journal Of Cell Biology 1994, 65: 327-40. PMID: 7536673.Peer-Reviewed Original ResearchConceptsClone 9 cellsEndocytic compartmentsPotential substrate proteinsDependent protein kinase IISynapsin ISmall intracellular vesiclesProtein kinase IIRat liver endosomesSubstrate proteinsPhosphorylation sequenceNon-neuronal cellsCLIP-170Intracellular vesiclesKinase IILarge endosomesPostnuclear supernatantEndosomesSensitive compartmentLiver endosomesProteinConfocal microscopyCell linesVesiclesIntestinal enterocytesCompartments
1990
Regulated phosphorylation of secretory granule membrane proteins of the rat parotid gland
Marino CR, Castle JD, Gorelick FS. Regulated phosphorylation of secretory granule membrane proteins of the rat parotid gland. American Journal Of Physiology 1990, 259: g70-g77. PMID: 1695488, DOI: 10.1152/ajpgi.1990.259.1.g70.Peer-Reviewed Original ResearchConceptsSecretory granule membrane proteinsIntegral membrane proteinsMembrane proteinsGranule membrane proteinProtein phosphorylation eventsGranule membrane fractionExocrine secretory granulesPhosphorylation eventsSodium dodecyl sulfate-polyacrylamide gel electrophoresisDodecyl sulfate-polyacrylamide gel electrophoresisRat parotid glandRegulated phosphorylationSulfate-polyacrylamide gel electrophoresisSitu phosphorylationIntact cellsMembrane fractionTime-dependent mannerPhosphorylationProteinSecretory granule fractionParotid lobulesGel electrophoresisProtein antiserumSecretory granulesPhosphoprotein
1989
Translocation of synapsin I in response to depolarization of isolated nerve terminals.
Sihra TS, Wang JK, Gorelick FS, Greengard P. Translocation of synapsin I in response to depolarization of isolated nerve terminals. Proceedings Of The National Academy Of Sciences Of The United States Of America 1989, 86: 8108-8112. PMID: 2510160, PMCID: PMC298224, DOI: 10.1073/pnas.86.20.8108.Peer-Reviewed Original ResearchConceptsSynapsin INerve terminalsRelease of neurotransmittersDepolarization of synaptosomesSmall synaptic vesiclesNeurotransmitter releaseSynaptic vesiclesDependent mannerIncubation mediumNerve terminal phosphoproteinDepolarizationPresent studyPhosphorylation stateCytosolic fractionPresence of Ca2TranslocationCa2Particulate fraction
1987
Calcium-Calmodulin-Dependent Protein Kinase in Hyperplastic Human Parathyroid Glands
KINDER BK, DELAHUNT NG, JAMIESON JD, GORELICK FS. Calcium-Calmodulin-Dependent Protein Kinase in Hyperplastic Human Parathyroid Glands. Endocrinology 1987, 120: 170-177. PMID: 3096699, DOI: 10.1210/endo-120-1-170.Peer-Reviewed Original Research