2020
Structural mechanism for replication origin binding and remodeling by a metazoan origin recognition complex and its co-loader Cdc6
Schmidt JM, Bleichert F. Structural mechanism for replication origin binding and remodeling by a metazoan origin recognition complex and its co-loader Cdc6. Nature Communications 2020, 11: 4263. PMID: 32848132, PMCID: PMC7450096, DOI: 10.1038/s41467-020-18067-7.Peer-Reviewed Original ResearchMeSH KeywordsAAA DomainAdenosine TriphosphateAnimalsCell Cycle ProteinsCryoelectron MicroscopyDNADrosophila melanogasterDrosophila ProteinsHydrolysisMinichromosome Maintenance ProteinsModels, MolecularOrigin Recognition ComplexProtein BindingRecombinant ProteinsReplication OriginSaccharomyces cerevisiaeSaccharomyces cerevisiae ProteinsConceptsOrigin recognition complexRecognition complexReplication originsDrosophila origin recognition complexEukaryotic DNA replication initiationMetazoan origin recognition complexCryo-electron microscopy structureMcm2-7 replicative helicaseATPase siteDNA replication initiationWalker B motifMcm2-7 loadingWinged-helix domainReplicative helicaseReplication initiationMicroscopy structureDistinct DNAB motifOrigin recognitionDNA sequencesDNA bendingDNA bindingPrimary DNADNA geometryLoop region
2019
Mechanisms of replication origin licensing: a structural perspective
Bleichert F. Mechanisms of replication origin licensing: a structural perspective. Current Opinion In Structural Biology 2019, 59: 195-204. PMID: 31630057, DOI: 10.1016/j.sbi.2019.08.007.Peer-Reviewed Reviews, Practice Guidelines, Standards, and Consensus StatementsConceptsReplicative helicasesKey cell cycle eventsReplication origin licensingReplication start sitesCell cycle eventsOrigin licensingReplicative helicaseReplication initiationInitiation factorsStart siteChromosomal DNAReplicative machineryCycle eventsMolecular approachesSynthesis apparatusHelicasesMachineryStructural perspectiveBidirectional mannerDNAEukaryotesHelicaseRecent advancesDuplicationMechanism
2015
Crystal structure of the eukaryotic origin recognition complex
Bleichert F, Botchan MR, Berger JM. Crystal structure of the eukaryotic origin recognition complex. Nature 2015, 519: 321-326. PMID: 25762138, PMCID: PMC4368505, DOI: 10.1038/nature14239.Peer-Reviewed Original ResearchConceptsOrigin recognition complexDrosophila origin recognition complexRecognition complexEukaryotic origin recognition complexHeterohexameric origin recognition complexMini-chromosome maintenance 2Winged-helix domainCatalytic amino acidsCellular DNA replicationWinged-helix foldORC subunitsEncircles DNAHelicase loadingGenomic integrityORC functionDevelopmental controlDNA replicationÅ resolutionDomain interactionsDisrupts interactionsCell cycleMaintenance 2Amino acidsCentral channelUnanticipated features
2009
A Dimeric Structure for Archaeal Box C/D Small Ribonucleoproteins
Bleichert F, Gagnon KT, Brown BA, Maxwell ES, Leschziner AE, Unger VM, Baserga SJ. A Dimeric Structure for Archaeal Box C/D Small Ribonucleoproteins. Science 2009, 325: 1384-1387. PMID: 19745151, PMCID: PMC2975540, DOI: 10.1126/science.1176099.Peer-Reviewed Original ResearchConceptsBox C/D sRNPArchaeal box C/D sRNPsRibosomal RNASingle-particle electron microscopyTarget ribosomal RNAArchaeon Methanocaldococcus jannaschiiOptimal protein synthesisRibose methylationMethanocaldococcus jannaschiiSmall ribonucleoproteinRNA componentEnzymatic functionSRNPDimeric structureMutational analysisProtein componentsProtein synthesisMethylationBase pairingRNAEukaryotesSnoRNPsArchaeaJannaschiiRibonucleoprotein