2015
CA‐074Me compound inhibits osteoclastogenesis via suppression of the NFATc1 and c‐FOS signaling pathways
Patel N, Nizami S, Song L, Mikami M, Hsu A, Hickernell T, Chandhanayingyong C, Rho S, Compton JT, Caldwell J, Kaiser PB, Bai H, Lee HG, Fischer CR, Lee FY. CA‐074Me compound inhibits osteoclastogenesis via suppression of the NFATc1 and c‐FOS signaling pathways. Journal Of Orthopaedic Research® 2015, 33: 1474-1486. PMID: 25428830, DOI: 10.1002/jor.22795.Peer-Reviewed Original ResearchConceptsOsteolytic disordersOsteoclast biologyBone resorptionCA-074MeC-FOSMechanisms of cathepsinsCathepsin B knockout miceB knockout miceCathepsin B inhibitor CA-074Dose-dependent mannerOsteoclast resorption pitsCathepsin B inhibitionInhibits osteoclastogenesisNFATc1 pathwayNew therapiesOsteoclastogenic effectsCA-074Knockout miceLysosomal proteasesMature osteoclastsResorption pitsCathepsin KNew targetsOsteoclastsCompound inhibits
2008
What are the local and systemic biologic reactions and mediators to wear debris, and what host factors determine or modulate the biologic response to wear particles?
Tuan RS, Lee FY, T Konttinen Y, Wilkinson JM, Smith RL. What are the local and systemic biologic reactions and mediators to wear debris, and what host factors determine or modulate the biologic response to wear particles? Journal Of The American Academy Of Orthopaedic Surgeons 2008, 16 Suppl 1: s42-8. PMID: 18612013, PMCID: PMC2714366, DOI: 10.5435/00124635-200800001-00010.Peer-Reviewed Original ResearchMeSH KeywordsBasic Helix-Loop-Helix Transcription FactorsBiocompatible MaterialsBone ResorptionDinoprostoneEndothelial CellsFibroblastsForeign-Body ReactionHumansInterleukin-1Interleukin-6Interleukin-8Joint ProsthesisMacrophage Colony-Stimulating FactorOsteoclastsOsteogenesisProsthesis FailureRANK LigandStem CellsT-LymphocytesTumor Necrosis Factor-alphaConceptsBone lossTNF-alphaIL-1Inflammatory bone lossPro-osteoclastogenic cytokinesGene functionHost inflammatory responsePeriprosthetic bone lossBone marrow-derived mesenchymal stem cellsMarrow-derived mesenchymal stem cellsGenetic variationBasic science dataMolecular mechanismsMesenchymal stem cellsMechanism of actionPeriprosthetic inflammationIL-6IL-8M-SCFInflammatory responseT cellsStem cellsAltered susceptibilityBiologic effectsOsteoprogenitor cells
2007
ERK Signaling Regulates Macrophage Colony‐Stimulating Factor Expression Induced by Titanium Particles in MC3T3.E1 Murine Calvarial Preosteoblastic Cells
SEO SW, LEE D, CHO SK, KIM AD, MINEMATSU H, AYDEMIR A, GELLER JA, MACAULAY W, YANG J, LEE F. ERK Signaling Regulates Macrophage Colony‐Stimulating Factor Expression Induced by Titanium Particles in MC3T3.E1 Murine Calvarial Preosteoblastic Cells. Annals Of The New York Academy Of Sciences 2007, 1117: 151-158. PMID: 18056041, DOI: 10.1196/annals.1402.027.Peer-Reviewed Original ResearchConceptsM-CSF expressionExtracellular signal-regulated kinase (ERK) pathwaySignal-regulated kinase pathwayPreosteoblastic cellsSignal transduction pathwaysSpecific ERK inhibitorReverse transcriptase-polymerase chain reactionEnzyme-linked immunosorbent assayPreosteoclast proliferationTransduction pathwaysKinase pathwayMolecular mechanismsMacrophage colony-stimulating factorCellular environmentERK inhibitorPeriprosthetic osteolysisM-CSF productionNuclear factor kappa beta ligandCell populationsBeta ligandSignificant clinical problemTranscriptase-polymerase chain reactionExpressionDose-dependent mannerColony-stimulating factor
2006
Mechanical Loading Differentially Regulates Membrane‐Bound and Soluble RANKL Availability in MC3T3‐E1 Cells
KIM DW, LEE H, KARMIN JA, LEE E, CHANG S, TOLCHIN B, LIN S, CHO SK, KWON A, AHN JM, LEE F. Mechanical Loading Differentially Regulates Membrane‐Bound and Soluble RANKL Availability in MC3T3‐E1 Cells. Annals Of The New York Academy Of Sciences 2006, 1068: 568-572. PMID: 16831954, DOI: 10.1196/annals.1346.054.Peer-Reviewed Original Research
2005
Zoledronate induces apoptosis in cells from fibro‐cellular membrane of unicameral bone cyst (UBC)
Yu J, Chang S, Suratwala S, Chung W, Abdelmessieh P, Lee H, Yang J, Lee F. Zoledronate induces apoptosis in cells from fibro‐cellular membrane of unicameral bone cyst (UBC). Journal Of Orthopaedic Research® 2005, 23: 1004-1012. PMID: 15921873, DOI: 10.1016/j.orthres.2005.02.012.Peer-Reviewed Original ResearchConceptsUnicameral bone cystsPeripheral blood mononuclear cellsBlood mononuclear cellsTrabecular bone cellsBone cystUBC cellsMononuclear cellsBone cellsZoledronate concentrationsIsolated peripheral blood mononuclear cellsImmunohisto/cytochemistryPercutaneous injection therapyAnti-resorptive agentsAnti-osteoclastogenic effectApoptotic fractionSTRO-1Potential therapeutic effectsPotential therapeutic roleEffect of zoledronateBenign cystic lesionsNitrogen-containing bisphosphonatesDose-dependent mannerOsteoclast-like cellsInjection therapyPathologic fractureμ-Calpain Regulates Receptor Activator of NF-κB Ligand (RANKL)-supported Osteoclastogenesis via NF-κB Activation in RAW 264.7 Cells*
Lee FY, Kim DW, Karmin JA, Hong D, Chang SS, Fujisawa M, Takayanagi H, Bigliani LU, Blaine TA, Lee HJ. μ-Calpain Regulates Receptor Activator of NF-κB Ligand (RANKL)-supported Osteoclastogenesis via NF-κB Activation in RAW 264.7 Cells*. Journal Of Biological Chemistry 2005, 280: 29929-29936. PMID: 15955824, DOI: 10.1074/jbc.m414600200.Peer-Reviewed Original ResearchConceptsRAW 264.7 cellsNF-kappaB activationReceptor activatorCalpain inhibitorsCell-permeable calpain inhibitorMatrix metalloproteinase-9Regulation of RANKLNF-κB activationNF-κB ligandNF-kappaB ligandMonocyte/macrophage progenitorsRole of calpainMurine RAW 264.7 cellsCell typesMetalloproteinase-9Osteoclastogenic markersCalpain activationResistant acidDecreased expressionOsteoclastogenesisRANKLCalpain activityMu-calpainInhibitorsActivation