2014
Eacpt-0032 TAT-HKII Induced Reduction in Mitochondrial Bound Hexokinase II Increases Ischemia Reperfusion Injury by Increased Respiration and Increased Ros Levels
Nederlof R, Gürel E, Xie C, Eerbeek O, Koeman A, Hollmann M, Southworth R, Akar F, Mik E, Zuurbier C. Eacpt-0032 TAT-HKII Induced Reduction in Mitochondrial Bound Hexokinase II Increases Ischemia Reperfusion Injury by Increased Respiration and Increased Ros Levels. Clinical Therapeutics 2014, 36: e13. DOI: 10.1016/j.clinthera.2014.05.051.Peer-Reviewed Original Research
2013
TAT-HKII induced reduction in mitochondrial bound hexokinase II increases ischemia reperfusion injury by increased respiration and increased ROS levels
Nederlof R, Guerel E, Xie C, Eerbeek O, Koeman A, Hollmann M, Southworth R, Akar F, Mik E, Zuurbier C. TAT-HKII induced reduction in mitochondrial bound hexokinase II increases ischemia reperfusion injury by increased respiration and increased ROS levels. European Heart Journal 2013, 34: 3694. DOI: 10.1093/eurheartj/eht309.3694.Peer-Reviewed Original ResearchMitochondrial oxygen tensionPeptide treatmentReperfusion injuryCardiac functionLDH activityROS productionControl groupHexokinase IIIschemia/reperfusion injuryOxygen consumptionIschemia-reperfusion injuryReactive oxygen speciesOxygen tensionCardiac oxygen consumptionCell deathBorderline ischemiaReperfusion necrosisR damageMin reperfusionReversible ischemiaIrreversible ischemiaMin ischemiaIschemiaTreatment groupsReperfusion
2011
07 Mitochondrial hexokinase II is essential for cardiac function and ischaemic preconditioning
Smeele K, Southworth R, Wu R, Xie C, Nederlof R, Warley A, Koeman A, Eerbeek O, Akar F, Ardehali H, Hollmann M, Zuurbier C. 07 Mitochondrial hexokinase II is essential for cardiac function and ischaemic preconditioning. Heart 2011, 97: e8. DOI: 10.1136/heartjnl-2011-301156.7.Peer-Reviewed Original ResearchIschemic preconditioningCardiac functionWT heartsProtective effectBaseline cardiac functionNormal cardiac functionMitochondrial permeability transition openingContractile impairmentIR injuryReperfusion injuryAcute reductionMyocardial functionGlycolytic enzymes hexokinaseMitochondrial hexokinase-IIEx vivoCardiac contractionMild mitochondrial uncouplingMitochondrial membrane depolarisationMembrane depolarisationHexokinase IIHKIIHeartMitochondrial swellingTissue disruptionInjuryDisruption of Hexokinase II–Mitochondrial Binding Blocks Ischemic Preconditioning and Causes Rapid Cardiac Necrosis
Smeele KM, Southworth R, Wu R, Xie C, Nederlof R, Warley A, Nelson JK, van Horssen P, van den Wijngaard JP, Heikkinen S, Laakso M, Koeman A, Siebes M, Eerbeek O, Akar FG, Ardehali H, Hollmann MW, Zuurbier CJ. Disruption of Hexokinase II–Mitochondrial Binding Blocks Ischemic Preconditioning and Causes Rapid Cardiac Necrosis. Circulation Research 2011, 108: 1165-1169. PMID: 21527739, DOI: 10.1161/circresaha.111.244962.Peer-Reviewed Original ResearchConceptsIschemic preconditioningWild-type heartsCardiac functionProtective effectHKII levelsBaseline cardiac functionIschemia-reperfusion injuryNormal cardiac functionMitochondrial permeability transition openingContractile impairmentReperfusion injuryAcute reductionCardiac necrosisMyocardial functionGlycolytic enzymes hexokinaseCardiac contractionMild mitochondrial uncouplingMembrane depolarizationMitochondrial membrane depolarizationHKIIMitochondrial hexokinaseControl peptideHeartPreconditioningTissue disruption