2016
Cholesterol Increases the Openness of SNARE-Mediated Flickering Fusion Pores
Stratton BS, Warner JM, Wu Z, Nikolaus J, Wei G, Wagnon E, Baddeley D, Karatekin E, O’Shaughnessy B. Cholesterol Increases the Openness of SNARE-Mediated Flickering Fusion Pores. Biophysical Journal 2016, 110: 1538-1550. PMID: 27074679, PMCID: PMC4833774, DOI: 10.1016/j.bpj.2016.02.019.Peer-Reviewed Original ResearchConceptsT-SNAREsFusion poreSoluble N-ethylmaleimide-sensitive factor attachment protein receptor (SNARE) proteinsLipid bilayer propertiesFactor attachment protein receptor proteinsN-ethylmaleimide-sensitive factor attachment protein receptor proteinsTotal internal reflection fluorescence microscopyCognate t-SNAREsFusion pore dynamicsPhysiological cholesterol levelsProtein receptor proteinsFlickering fusion poresReflection fluorescence microscopyIndividual fusion eventsV-SNAREBilayer propertiesFusion eventsPore flickeringReceptor proteinFluorescence microscopySmall unilamellar vesiclesLipid compositionFusion siteSnareExocytotic release
2010
A fast, single-vesicle fusion assay mimics physiological SNARE requirements
Karatekin E, Di Giovanni J, Iborra C, Coleman J, O'Shaughnessy B, Seagar M, Rothman JE. A fast, single-vesicle fusion assay mimics physiological SNARE requirements. Proceedings Of The National Academy Of Sciences Of The United States Of America 2010, 107: 3517-3521. PMID: 20133592, PMCID: PMC2840481, DOI: 10.1073/pnas.0914723107.Peer-Reviewed Original Research