2010
Actin filament remodeling by actin depolymerization factor/cofilin
Pfaendtner J, De La Cruz EM, Voth GA. Actin filament remodeling by actin depolymerization factor/cofilin. Proceedings Of The National Academy Of Sciences Of The United States Of America 2010, 107: 7299-7304. PMID: 20368459, PMCID: PMC2867716, DOI: 10.1073/pnas.0911675107.Peer-Reviewed Original ResearchConceptsActin depolymerization factorSubunit interactionsCofilin bindingActin filamentsADF/cofilinFactor/cofilinLong-pitch helixHelix monomersNeighboring subunitMolecular basisSevering proteinHydrophobic loopConformational dynamicsSubdomain 1Subdomain 2Loop movesTriggers reorganizationCofilinFilament flexibilityFilamentsNative filamentsDNaseFilament contactsAtom simulationsBinding
2008
Cofilin Increases the Bending Flexibility of Actin Filaments: Implications for Severing and Cell Mechanics
McCullough BR, Blanchoin L, Martiel JL, De La Cruz EM. Cofilin Increases the Bending Flexibility of Actin Filaments: Implications for Severing and Cell Mechanics. Journal Of Molecular Biology 2008, 381: 550-558. PMID: 18617188, PMCID: PMC2753234, DOI: 10.1016/j.jmb.2008.05.055.Peer-Reviewed Original Research
2000
Thymosin-β4 Changes the Conformation and Dynamics of Actin Monomers
De La Cruz E, Ostap E, Brundage R, Reddy K, Sweeney H, Safer D. Thymosin-β4 Changes the Conformation and Dynamics of Actin Monomers. Biophysical Journal 2000, 78: 2516-2527. PMID: 10777749, PMCID: PMC1300842, DOI: 10.1016/s0006-3495(00)76797-x.Peer-Reviewed Original ResearchMeSH KeywordsActinsAnimalsBinding SitesBiophysical PhenomenaBiophysicsCircular DichroismCross-Linking ReagentsFluorescent DyesHumansIn Vitro TechniquesKineticsMacromolecular SubstancesModels, MolecularMuscle, SkeletalMutagenesis, Site-DirectedNaphthalenesulfonatesOsmotic PressureProtein BindingProtein ConformationRabbitsRecombinant ProteinsThermodynamicsThymosinTritiumViscosityConceptsActin monomersActin monomer poolMgATP-actinMetazoan cellsNucleotide exchangeC-terminusSubdomain 2Conformational changesMonomer poolN-iodoacetyl-N'Cleavage siteActinThymosin β4Rate of dissociationAmide protonsSulfo-1Unique abilityBindingStructural dynamicsConformationTerminusNucleotidesProteolysisDifferent stabilitiesDegrees C