2016
Isolation and Culture of Adult Zebrafish Brain-derived Neurospheres.
Lopez-Ramirez MA, Calvo CF, Ristori E, Thomas JL, Nicoli S. Isolation and Culture of Adult Zebrafish Brain-derived Neurospheres. Journal Of Visualized Experiments 2016, 53617. PMID: 26967835, PMCID: PMC4828210, DOI: 10.3791/53617.Peer-Reviewed Original ResearchConceptsAdult zebrafish brainStem/progenitor cellsZebrafish brainMolecular mechanismsRelevant model organismZebrafish adult brainProgenitor cellsSingle-cell dissociationSpecific signaling pathwaysAdult stem/progenitor cellsStem/progenitor cell proliferationNeural stem/progenitor cell proliferationProgenitor cell proliferationNeural stem/progenitor cellsModel organismsGene expressionCell dissociationSignaling pathwaysMultipotent neural stem/progenitor cellsNeurosphere assayAdult neurogenesisCell proliferationZebrafishBrain regenerationAdult brain
2015
Vascular Mural Cells Promote Noradrenergic Differentiation of Embryonic Sympathetic Neurons
Fortuna V, Pardanaud L, Brunet I, Ola R, Ristori E, Santoro MM, Nicoli S, Eichmann A. Vascular Mural Cells Promote Noradrenergic Differentiation of Embryonic Sympathetic Neurons. Cell Reports 2015, 11: 1786-1796. PMID: 26074079, DOI: 10.1016/j.celrep.2015.05.028.Peer-Reviewed Original ResearchConceptsPlatelet-derived growth factor receptorNA differentiationNoradrenergic differentiationMural cell recruitmentDorsal aortaSympathetic neuronsCloche mutantsCell recruitmentZebrafish embryosEmbryonic sympathetic neuronsGrowth factor receptorVascular mural cellsSmooth muscle targetsPostganglionic sympathetic neuronsSympathetic nervous systemMural cellsSympathetic precursorsFactor receptorVascular maturationDifferentiationMuscle toneMuscle targetsNeurovascular interactionsHeart rateNervous system