2010
Mouse cystic fibrosis transmembrane conductance regulator forms cAMP-PKA–regulated apical chloride channels in cortical collecting duct
Lu M, Dong K, Egan ME, Giebisch GH, Boulpaep EL, Hebert SC. Mouse cystic fibrosis transmembrane conductance regulator forms cAMP-PKA–regulated apical chloride channels in cortical collecting duct. Proceedings Of The National Academy Of Sciences Of The United States Of America 2010, 107: 6082-6087. PMID: 20231442, PMCID: PMC2851921, DOI: 10.1073/pnas.0902661107.Peer-Reviewed Original ResearchMeSH KeywordsAnimalsBenzoatesChloride ChannelsCyclic AMPCyclic AMP-Dependent Protein KinasesCystic Fibrosis Transmembrane Conductance RegulatorFemaleIn Vitro TechniquesKidney CortexKidney Tubules, CollectingKineticsMiceMice, Inbred C57BLMice, Inbred CFTRMice, KnockoutMice, TransgenicMutationOocytesPatch-Clamp TechniquesPotassium Channels, Inwardly RectifyingRecombinant ProteinsThiazolidinesXenopus laevisConceptsCystic fibrosis transmembrane conductance regulatorFibrosis transmembrane conductance regulatorTransmembrane conductance regulatorCl- channel activityConductance regulatorCl- channelsApical membrane proteinsExpression of CFTRChannel activityCFTR Cl- channelApical chloride channelApical cell membraneDeltaF508 CFTR mutationMembrane proteinsCatalytic subunitXenopus laevis oocytesForm proteinPrincipal cellsCFTR channelsROMK null miceApical patchesApical membraneSingle-channel conductanceChloride channelsCell membrane
1998
Properties of an Inwardly Rectifying ATP-sensitive K+ Channel in the Basolateral Membrane of Renal Proximal Tubule
Mauerer U, Boulpaep E, Segal A. Properties of an Inwardly Rectifying ATP-sensitive K+ Channel in the Basolateral Membrane of Renal Proximal Tubule. The Journal Of General Physiology 1998, 111: 139-160. PMID: 9417141, PMCID: PMC1887768, DOI: 10.1085/jgp.111.1.139.Peer-Reviewed Original ResearchMeSH KeywordsAdenosine TriphosphateAmbystomaAnimalsBariumBiological TransportCationsCells, CulturedCytidine TriphosphateDiazoxideDiureticsElectric ConductivityGlyburideGuanosine TriphosphateHypoglycemic AgentsInosine TriphosphateIon Channel GatingKidney Tubules, ProximalKineticsMicrovilliPatch-Clamp TechniquesPotassium ChannelsSodium Chloride Symporter InhibitorsSodium-Potassium-Exchanging ATPaseThalliumThymine NucleotidesUridine TriphosphateConceptsProximal tubule cellsProximal tubulesTubule cellsBasolateral membraneChannel opener diazoxideChannel activityPump-leak couplingRenal proximal tubulesPivotal physiological rolesOpener diazoxideMillimolar dosesMM ATPHigh dosesPotassium conductanceCell membrane potentialInternal Mg2Cytosolic additionATPase pump
1987
Electrochemical analysis of renal Na+-glucose cotransport in salamander proximal tubules
Morgunov N, Boulpaep E. Electrochemical analysis of renal Na+-glucose cotransport in salamander proximal tubules. American Journal Of Physiology 1987, 252: f154-f169. PMID: 3812699, DOI: 10.1152/ajprenal.1987.252.1.f154.Peer-Reviewed Original ResearchConceptsLuminal glucoseProximal tubulesFavorable chemical gradientFavorable electrical gradientSodium concentrationSodium-glucose cotransporterIntracellular sodium activityElectrogenic responseSodium activityMM D-glucoseMembrane potentialSalamander proximal tubuleLuminal perfusateOmega X cm2Glucose cotransportGlucose concentrationLuminal membranePhysiological rangeX cm2Luminal membrane potentialGlucoseD-glucoseTubulesLuminal membrane resistancePerfusate
1984
Single channel recordings of calcium-activated potassium channels in the apical membrane of rabbit cortical collecting tubules.
Hunter M, Lopes A, Boulpaep E, Giebisch G. Single channel recordings of calcium-activated potassium channels in the apical membrane of rabbit cortical collecting tubules. Proceedings Of The National Academy Of Sciences Of The United States Of America 1984, 81: 4237-4239. PMID: 6330754, PMCID: PMC345404, DOI: 10.1073/pnas.81.13.4237.Peer-Reviewed Original ResearchConceptsPotassium channelsRabbit cortical collecting tubuleCalcium concentrationCalcium-activated potassium channelsDose-dependent fashionCortical collecting tubulePatch-clamp techniqueCollecting tubuleSingle potassium channelsApical membraneOpen channel probabilitySingle-channel recordingsSlope conductanceChannel activityTubulesChannel recordingsRecordings
1982
Electrical properties of chloride transport across theNecturus proximal tubule
Guggino W, Boulpaep E, Giebisch G. Electrical properties of chloride transport across theNecturus proximal tubule. The Journal Of Membrane Biology 1982, 65: 185-196. PMID: 7062339, DOI: 10.1007/bf01869962.Peer-Reviewed Original ResearchIonic conductive properties and electrophysiology of the rabbit cortical collecting tubule
O'Neil R, Boulpaep E. Ionic conductive properties and electrophysiology of the rabbit cortical collecting tubule. American Journal Of Physiology 1982, 243: f81-f95. PMID: 7091368, DOI: 10.1152/ajprenal.1982.243.1.f81.Peer-Reviewed Original Research
1981
Isolated perfused salamander proximal tubule. II. Monovalent ion replacement and rheogenic transport
Sackin H, Boulpaep E. Isolated perfused salamander proximal tubule. II. Monovalent ion replacement and rheogenic transport. American Journal Of Physiology 1981, 241: f540-f555. PMID: 7304748, DOI: 10.1152/ajprenal.1981.241.5.f540.Peer-Reviewed Original ResearchThe rheogenic nature of sodium transport in leaky epithelia studied by equivalent circuit analysis.
Sackin H, Boulpaep E. The rheogenic nature of sodium transport in leaky epithelia studied by equivalent circuit analysis. Progress In Clinical And Biological Research 1981, 73: 57-66. PMID: 6275400.Peer-Reviewed Original Research
1979
Effect of amiloride on the apical cell membrane cation channels of a sodium-absorbing, potassium-secreting renal epithelium
O'Neil R, Boulpaep E. Effect of amiloride on the apical cell membrane cation channels of a sodium-absorbing, potassium-secreting renal epithelium. The Journal Of Membrane Biology 1979, 50: 365-387. PMID: 513119, DOI: 10.1007/bf01868898.Peer-Reviewed Original ResearchConceptsLumen-negative transepithelial potential differenceActive Na absorptionApical cell membraneEffects of amilorideEquivalent short-circuit currentTransepithelial potential differenceNa absorptionMaximum inhibitory concentrationAmilorideDiuretic amilorideCation channelsCell membraneNa channelsInhibitory concentrationRenal epitheliumMembrane cation channelsRemoval of NaAbsence of NaTight junctionsPerfusateK permeabilityTubulesLumen