A Cysteine Variant at an Allosteric Site Alters MIF Dynamics and Biological Function in Homo- and Heterotrimeric Assemblies
Skeens E, Pantouris G, Shah D, Manjula R, Ombrello MJ, Maluf NK, Bhandari V, Lisi GP, Lolis EJ. A Cysteine Variant at an Allosteric Site Alters MIF Dynamics and Biological Function in Homo- and Heterotrimeric Assemblies. Frontiers In Molecular Biosciences 2022, 9: 783669. PMID: 35252348, PMCID: PMC8893199, DOI: 10.3389/fmolb.2022.783669.Peer-Reviewed Original ResearchAllosteric siteNon-overlapping functionsEnzymatic activityHeterotrimeric assemblyBiological functionsWild typeCatalytic baseCysteine variantsFunctional interactionHuman macrophage migration inhibitory factorSolvent channelsMacrophage migration inhibitory factorEnzymatic cavityCrystallographic structureNMR dynamicsMixed wild typeVariantsY99CInhibitory factorMammalsNucleaseSubunitsCytosolDifferent extentsFish