2016
The Human Adrenal Gland Proteome Defined by Transcriptomics and Antibody-Based Profiling
Bergman J, Botling J, Fagerberg L, Hallström B, Djureinovic D, Uhlén M, Pontén F. The Human Adrenal Gland Proteome Defined by Transcriptomics and Antibody-Based Profiling. Endocrinology 2016, 158: 239-251. PMID: 27901589, DOI: 10.1210/en.2016-1758.Peer-Reviewed Original ResearchConceptsImmunohistochemistry-based protein profilingNormal human tissue typesProtein coding genesMessenger RNA sequencingExpression levelsProtein expression patternsHuman tissue typesCoding genesProtein homologsFERM domainRelative expression levelsCorresponding proteinRNA sequencingWide transcriptomicsExpression patternsProtein profilingGreater expression levelsGenesSpecific functionsProteinMolecular landscapeTissue typesProteomeTranscriptomicsElevated expression
2015
Tissue-based map of the human proteome
Uhlén M, Fagerberg L, Hallström B, Lindskog C, Oksvold P, Mardinoglu A, Sivertsson Å, Kampf C, Sjöstedt E, Asplund A, Olsson I, Edlund K, Lundberg E, Navani S, Szigyarto C, Odeberg J, Djureinovic D, Takanen J, Hober S, Alm T, Edqvist P, Berling H, Tegel H, Mulder J, Rockberg J, Nilsson P, Schwenk J, Hamsten M, von Feilitzen K, Forsberg M, Persson L, Johansson F, Zwahlen M, von Heijne G, Nielsen J, Pontén F. Tissue-based map of the human proteome. Science 2015, 347: 1260419. PMID: 25613900, DOI: 10.1126/science.1260419.Peer-Reviewed Original ResearchConceptsPutative protein-coding genesHuman tissue proteomesProtein-coding genesInteractive web-based databaseIntegrated omics approachDifferent tissuesGlobal expression patternsSingle-cell levelMembrane proteomeProteome variationDruggable proteomeOmics approachesHuman proteomeHuman secretomeMolecular detailsIndividual proteinsQuantitative transcriptomicsCancer proteomeTissue proteomeProteomeExpression patternsHuman biologyMetabolic functionsTissue microarray-based immunohistochemistryMajor tissues
2014
The human testis-specific proteome defined by transcriptomics and antibody-based profiling
Djureinovic D, Fagerberg L, Hallström B, Danielsson A, Lindskog C, Uhlén M, Pontén F. The human testis-specific proteome defined by transcriptomics and antibody-based profiling. Molecular Human Reproduction 2014, 20: 476-488. PMID: 24598113, DOI: 10.1093/molehr/gau018.Peer-Reviewed Original ResearchConceptsExpression patternsAntibody-based protein profilingTestis-enriched expression patternNormal human tissue typesWide transcriptomic analysisTissue-specific genesAntibody-based profilingPutative human genesList of genesHaploid germ cellsHuman tissue typesDifferent cell typesHuman reproductive biologyReproductive biologyWide analysisProtein localizationHuman genesTranscriptomic analysisCorresponding proteinStages of spermatogenesisDeep sequencingProtein profilingMolecular understandingGerm cellsGenes
2013
Analysis of the Human Tissue-specific Expression by Genome-wide Integration of Transcriptomics and Antibody-based Proteomics*
Fagerberg L, Hallström B, Oksvold P, Kampf C, Djureinovic D, Odeberg J, Habuka M, Tahmasebpoor S, Danielsson A, Edlund K, Asplund A, Sjöstedt E, Lundberg E, Szigyarto C, Skogs M, Takanen J, Berling H, Tegel H, Mulder J, Nilsson P, Schwenk J, Lindskog C, Danielsson F, Mardinoglu A, Sivertsson Å, von Feilitzen K, Forsberg M, Zwahlen M, Olsson I, Navani S, Huss M, Nielsen J, Ponten F, Uhlén M. Analysis of the Human Tissue-specific Expression by Genome-wide Integration of Transcriptomics and Antibody-based Proteomics*. Molecular & Cellular Proteomics 2013, 13: 397-406. PMID: 24309898, PMCID: PMC3916642, DOI: 10.1074/mcp.m113.035600.Peer-Reviewed Original ResearchConceptsHuman protein-coding genesProtein-coding genesSpatial expression patternsTissue-specific expressionExpression patternsGenome-wide integrationIndividual gene levelAntibody-based profilingQuantitative transcriptomic analysisProtein expression dataHuman Protein AtlasMajor human organsTranscriptomic analysisHuman proteinsExpression mapProtein AtlasGene levelExpression dataHuman biologyProtein analysisGenesMolecular constituentsSame tissueRNAExpression