2002
A one‐step method for in vitro production of tRNA transcripts
Korencić D, Söll D, Ambrogelly A. A one‐step method for in vitro production of tRNA transcripts. Nucleic Acids Research 2002, 30: e105-e105. PMID: 12384607, PMCID: PMC137149, DOI: 10.1093/nar/gnf104.Peer-Reviewed Original ResearchMeSH KeywordsDNADNA-Directed RNA PolymerasesDNA, Single-StrandedGenetic TechniquesRNA, TransferTemplates, GeneticTranscription, GeneticViral ProteinsConceptsTRNA transcriptsT7 RNA polymeraseLarge-scale plasmid preparationTRNA genesMicrobial genomesTRNA functionsDNA promoterRNA polymeraseRNA moleculesT7 promoterBiochemical characterizationTranscription templateDNA templateNew enzymeTranscriptsLarge oligonucleotidesTranscriptionGood substratePromoterShort oligonucleotidesEnzymatic digestionRapid productionPlasmid preparationsGenomeOligonucleotide
1987
Simplified in vitro synthesis of mutated RNA molecules
Krupp G, Söll D. Simplified in vitro synthesis of mutated RNA molecules. FEBS Letters 1987, 212: 271-275. PMID: 3545903, DOI: 10.1016/0014-5793(87)81359-5.Peer-Reviewed Original Research