1987
Cloning and characterization of the gene coding for cytoplasmic seryl-tRNA synthetase from Saccharomyces cerevisiae
Weygand-Durasevic I, johnson-Burke D, Söll D. Cloning and characterization of the gene coding for cytoplasmic seryl-tRNA synthetase from Saccharomyces cerevisiae. Nucleic Acids Research 1987, 15: 1887-1904. PMID: 3031581, PMCID: PMC340606, DOI: 10.1093/nar/15.5.1887.Peer-Reviewed Original ResearchConceptsSeryl-tRNA synthetaseSingle open reading frameAbundant yeast proteinsGenomic Southern blotsNuclease S1 analysisOpen reading frameTranslation initiation codonAmino acid sequenceKb SalI fragmentNucleotide sequence analysisAT-rich sequencesYeast proteinsStructural geneCodon usageS1 analysisTATA boxInitiation codonReading frameSer geneSalI fragmentAcid sequenceExpression librarySequence analysisRich sequencesSouthern blot
1985
A wheat HMW glutenin subunit gene reveals a highly repeated structure
Sugiyama T, Rafalski A, Peterson D, Söll D. A wheat HMW glutenin subunit gene reveals a highly repeated structure. Nucleic Acids Research 1985, 13: 8729-8737. PMID: 3001648, PMCID: PMC318947, DOI: 10.1093/nar/13.24.8729.Peer-Reviewed Original ResearchConceptsTypical eukaryotic promoterWheat genomic libraryHMW glutenin subunit genesRNA initiation siteGlutenin subunit genesPartial cDNA cloneAmino acid sequenceMature proteinGenomic libraryGlutenin genesEukaryotic promotersCDNA clonesGene sequencesProline contentSubunit geneAcid sequenceGrain endospermInitiation siteQuality of wheatGenesSynthetic oligonucleotidesHigh glutamineMolecular weightProteinClonesLeucine tRNA family of Escherichia coli: nucleotide sequence of the supP(Am) suppressor gene
Thorbjarnardóttir S, Dingermann T, Rafnar T, Andrésson O, Söll D, Eggertsson G. Leucine tRNA family of Escherichia coli: nucleotide sequence of the supP(Am) suppressor gene. Journal Of Bacteriology 1985, 161: 219-222. PMID: 2981802, PMCID: PMC214859, DOI: 10.1128/jb.161.1.219-222.1985.Peer-Reviewed Original ResearchConceptsSuppressor allelesLeuX geneAmber suppressor allelesMature coding sequenceLeucyl-tRNA synthetaseSingle base changeTRNA familiesCAA anticodonBox sequenceTermination signalDNA sequencesNucleotide sequenceBacteriophage T4Coding sequenceAminoacyl stemSuppressor geneLoop regionTRNABase changesEscherichia coliGenesE. coliSequenceColiAlleles
1984
Transcriptionally active and inactive gene repeats within the D. meianogaster 5S RNA gene cluster
Sharp S, Garcia A, Cooley L, Söll D. Transcriptionally active and inactive gene repeats within the D. meianogaster 5S RNA gene cluster. Nucleic Acids Research 1984, 12: 7617-7632. PMID: 6093044, PMCID: PMC320189, DOI: 10.1093/nar/12.20.7617.Peer-Reviewed Original ResearchConceptsEfficiency of transcriptionRRNA gene copiesHigh transcription efficiencyTwo-nucleotide deletionD. melanogasterGene repeatRNA genesGene clusterPrimary transcriptGene copiesTranscription functionTranscription efficiencyTemplate activityCell extractsTranscriptionDNAPosition 28Position 86DNA typesRepeat unitsDeletionSame sequenceMelanogasterRRNAGenesIn vivo and in vitro transcription of the Escherichia coli glutaminyl-tRNA synthetase gene.
Cheung A, Söll D. In vivo and in vitro transcription of the Escherichia coli glutaminyl-tRNA synthetase gene. Journal Of Biological Chemistry 1984, 259: 9953-9958. PMID: 6086662, DOI: 10.1016/s0021-9258(17)42791-8.Peer-Reviewed Original ResearchConceptsGlutaminyl-tRNA synthetaseGlutaminyl-tRNA synthetase geneTranslation initiation codon AUGInitiation codon AUGPresence of tRNATermination codon UAAGlnS geneTerminator structureTranscription initiationSynthetase geneTranscription initiatesCodons UAARegulatory signalsCodon AUGTermination sitesTranscription productsSequence analysisStructure upstreamStructural regionsTranscriptionGenesTranscriptsSynthetaseMRNAGlnDevelopmentally regulated plant genes: the nucleotide sequence of a wheat gliadin genomic clone.
Rafalski J, Scheets K, Metzler M, Peterson D, Hedgcoth C, Söll D. Developmentally regulated plant genes: the nucleotide sequence of a wheat gliadin genomic clone. The EMBO Journal 1984, 3: 1409-1415. PMID: 6204862, PMCID: PMC557531, DOI: 10.1002/j.1460-2075.1984.tb01985.x.Peer-Reviewed Original ResearchConceptsCDNA clonesGenomic clonesWheat genomic libraryWheat seed storage proteinsInternal sequence repetitionSeed storage proteinsSuch genomic clonesTranslation termination codonEukaryotic promoter sequencesPlant genesNorthern blot analysisMultigene familyGliadin genesGenomic libraryHybrid-selected mRNA translationMRNA translationCDNA sequenceGene sequencesPromoter sequencesCDNA libraryStorage proteinsDNA sequencesNucleotide sequenceNumber of clonesTermination codon
1982
Escherichia coli glutaminyl-tRNA synthetase. I. Isolation and DNA sequence of the glnS gene.
Yamao F, Inokuchi H, Cheung A, Ozeki H, Söll D. Escherichia coli glutaminyl-tRNA synthetase. I. Isolation and DNA sequence of the glnS gene. Journal Of Biological Chemistry 1982, 257: 11639-11643. PMID: 6288695, DOI: 10.1016/s0021-9258(18)33810-9.Peer-Reviewed Original ResearchMeSH KeywordsAmino Acyl-tRNA SynthetasesBacteriophage lambdaBase SequenceDNA Restriction EnzymesDNA, BacterialEscherichia coliGenesMutationPlasmidsTransduction, GeneticArrangement of the ribosomal RNA genes in Schizosaccharomyces pombe
Barnitz J, Cramer J, Rownd R, Cooley L, Söll D. Arrangement of the ribosomal RNA genes in Schizosaccharomyces pombe. FEBS Letters 1982, 143: 129-132. PMID: 6288447, DOI: 10.1016/0014-5793(82)80288-3.Peer-Reviewed Original ResearchThe 5.8S RNA gene sequence and the ribosomal repeat of Schizosaccharomyces pombe
Schaak J, Mao J, Söll D. The 5.8S RNA gene sequence and the ribosomal repeat of Schizosaccharomyces pombe. Nucleic Acids Research 1982, 10: 2851-2864. PMID: 6285312, PMCID: PMC320660, DOI: 10.1093/nar/10.9.2851.Peer-Reviewed Original ResearchMeSH KeywordsAscomycotaDNA Restriction EnzymesGenesNucleic Acid ConformationNucleic Acid HybridizationRepetitive Sequences, Nucleic AcidRNA, RibosomalSchizosaccharomycesConceptsRNA genesSchizosaccharomyces pombeRNA speciesGene sequencesDNA fragmentsKb HindIII DNA fragmentRRNA gene repeatsRNA gene sequencesKb DNA fragmentHindIII DNA fragmentSecondary structure modelNumber of nucleotidesHigher eukaryotesGene repeatIntergenic spacerN. crassaRibosomal repeatNucleotide sequenceS. cerevisiaeGreatest homologySequence differencesVariable stemGenesRNARepeatsGenes for tRNA 5Lys from Drosophila melanogaster
DeFranco D, Burke K, Hayashi S, Tener G, Miller R, Söll D. Genes for tRNA 5Lys from Drosophila melanogaster. Nucleic Acids Research 1982, 10: 5799-5808. PMID: 6292853, PMCID: PMC320931, DOI: 10.1093/nar/10.19.5799.Peer-Reviewed Original ResearchThe 5S RNA genes of Schizosaccharomyces pombe
Mao J, Appel B, Schaack J, Sharp S, Yamada H, Söll D. The 5S RNA genes of Schizosaccharomyces pombe. Nucleic Acids Research 1982, 10: 487-500. PMID: 6278416, PMCID: PMC326152, DOI: 10.1093/nar/10.2.487.Peer-Reviewed Original Research
1981
The initiator tRNA genes of Drosophila melanogaster: evidence for a tRNA pseudogene
Sharp S, DeFranco D, Silberklang M, Hosbach H, Schmidt T, Kubli E, Gergen J, Wensink P, Söll D. The initiator tRNA genes of Drosophila melanogaster: evidence for a tRNA pseudogene. Nucleic Acids Research 1981, 9: 5867-5882. PMID: 6273811, PMCID: PMC327570, DOI: 10.1093/nar/9.22.5867.Peer-Reviewed Original ResearchConceptsInitiator tRNA geneTRNA genesDrosophila genomeDrosophila melanogasterTRNA gene familyRNA polymerase IIIDrosophila melanogaster DNAKb DNA fragmentTRNA pseudogenesRepetitive DNAGene familyPolymerase IIISingle copyGenomic DNADNA fragmentsPseudogenesRepeat segmentsGenesHybridization analysisFourth cloneMelanogasterSitu hybridizationGenomeDNAFragments