2023
Recoding UAG to selenocysteine in Saccharomyces cerevisiae
Hoffman K, Chung C, Mukai T, Krahn N, Jiang H, Balasuriya N, O'Donoghue P, Söll D. Recoding UAG to selenocysteine in Saccharomyces cerevisiae. RNA 2023, 29: 1400-1410. PMID: 37279998, PMCID: PMC10573291, DOI: 10.1261/rna.079658.123.Peer-Reviewed Original ResearchMeSH KeywordsAeromonas salmonicidaCodon, TerminatorHumansNucleic Acid ConformationProtein EngineeringRNA, Transfer, CysSaccharomyces cerevisiaeConceptsSelenoprotein productionYeast expression systemSeryl-tRNA synthetaseSite-specific incorporationEukaryotic relativesKingdom FungiSelenocysteine synthaseSelenophosphate synthetaseBiosynthesis pathwayEukaryotic selenoproteinsMetabolic engineeringBiosynthetic pathwayPathway componentsExpression systemReductase enzymeTRNASaccharomycesYeastTranslation componentsSpecific sitesFacile productionUnique chemicalSynthetasePathwayFirst demonstration
1997
When protein engineering confronts the tRNA world
Schimmel P, Söll D. When protein engineering confronts the tRNA world. Proceedings Of The National Academy Of Sciences Of The United States Of America 1997, 94: 10007-10009. PMID: 9294151, PMCID: PMC33764, DOI: 10.1073/pnas.94.19.10007.Peer-Reviewed Original Research