2000
Ancient Adaptation of the Active Site of Tryptophanyl-tRNA Synthetase for Tryptophan Binding †
Ibba M, Stange-Thomann N, Kitabatake M, Ali K, Söll I, Carter, C, Michael Ibba, and, Söll D. Ancient Adaptation of the Active Site of Tryptophanyl-tRNA Synthetase for Tryptophan Binding †. Biochemistry 2000, 39: 13136-13143. PMID: 11052665, DOI: 10.1021/bi001512t.Peer-Reviewed Original ResearchMeSH KeywordsAcylationAnimalsBacillus subtilisBacterial ProteinsBinding SitesCattleDiphosphatesDNA Mutational AnalysisDNA, BacterialEvolution, MolecularGeobacillus stearothermophilusHumansKineticsMiceMutagenesis, Site-DirectedProtein BindingRabbitsRNA, Transfer, TrpSequence Homology, Amino AcidTryptophanTryptophan-tRNA LigaseTyrosineConceptsAmino acid specificityActive site residuesTyrosyl-tRNA synthetasesTryptophanyl-tRNA synthetaseAncient adaptationAnalogous residuesGlu side chainsTryptophan replacementHomologous positionsSystematic mutationAromatic side chainsTrpRSTryptophan recognitionBacillus stearothermophilusSide chainsTryptophan bindingTyrRSResiduesCommon originCompetitive inhibitorMutationsTrp bindingMechanistic supportCatalytic efficiencyActive site
1999
Transfer RNA identity contributes to transition state stabilization during aminoacyl-tRNA synthesis
Ibba M, Sever S, Praetorius-Ibba M, Söll D. Transfer RNA identity contributes to transition state stabilization during aminoacyl-tRNA synthesis. Nucleic Acids Research 1999, 27: 3631-3637. PMID: 10471730, PMCID: PMC148616, DOI: 10.1093/nar/27.18.3631.Peer-Reviewed Original Research
1995
A broadly applicable continuous spectrophotometric assay for measuring aminoacyl-tRNA synthetase activity
Lloyd A, Thomann H, Ibba M, Soöll D. A broadly applicable continuous spectrophotometric assay for measuring aminoacyl-tRNA synthetase activity. Nucleic Acids Research 1995, 23: 2886-2892. PMID: 7659511, PMCID: PMC307126, DOI: 10.1093/nar/23.15.2886.Peer-Reviewed Original Research
1992
Aminoacyl-tRNA synthetase-induced cleavage of tRNA
Beresten S, Jahn M, Söll D. Aminoacyl-tRNA synthetase-induced cleavage of tRNA. Nucleic Acids Research 1992, 20: 1523-1530. PMID: 1579445, PMCID: PMC312233, DOI: 10.1093/nar/20.7.1523.Peer-Reviewed Original Research