1984
The extent of a eukaryotic tRNA gene. 5‘- and 3‘-flanking sequence dependence for transcription and stable complex formation.
Schaack J, Sharp S, Dingermann T, Burke DJ, Cooley L, Söll D. The extent of a eukaryotic tRNA gene. 5‘- and 3‘-flanking sequence dependence for transcription and stable complex formation. Journal Of Biological Chemistry 1984, 259: 1461-1467. PMID: 6693417, DOI: 10.1016/s0021-9258(17)43429-6.Peer-Reviewed Original ResearchConceptsStable complex formationBase pairsDrosophila Kc cell extractSequence requirementsCell extractsEukaryotic tRNA genesStable transcription complexesHeLa cell extractsTRNA genesComplex formationTranscription complexArg genesEfficient transcriptionTranscription assaysTranscription propertiesCell-free extractsTranscriptionHomologous systemGenesSequenceSequence dependenceCellular sourceExtractAssaysPairs
1983
Stable transcription complex formation of eukaryotic tRNA genes is dependent on a limited separation of the two intragenic control regions.
Dingermann T, Sharp S, Schaack J, Söll D. Stable transcription complex formation of eukaryotic tRNA genes is dependent on a limited separation of the two intragenic control regions. Journal Of Biological Chemistry 1983, 258: 10395-10402. PMID: 6309803, DOI: 10.1016/s0021-9258(17)44470-x.Peer-Reviewed Original ResearchConceptsIntragenic control regionControl regionTRNA genesTRNAArg geneDrosophila Kc cell extractStable transcription complex formationTranscription efficiencyDrosophila tRNAArg geneT-control regionMutant tRNA genesEukaryotic tRNA genesTranscription complex formationStable transcription complexesWild-type geneXhoI linkerTranscriptional roleTranscription initiationTranscription complexDNA regionsType genesTranscription factorsGene transcriptionTermination sitesDNA fragmentsCell extractsTranscription of eukaryotic tRNA genes in vitro. II. Formation of stable complexes.
Schaack J, Sharp S, Dingermann T, Söll D. Transcription of eukaryotic tRNA genes in vitro. II. Formation of stable complexes. Journal Of Biological Chemistry 1983, 258: 2447-2453. PMID: 6549758, DOI: 10.1016/s0021-9258(18)32946-6.Peer-Reviewed Original ResearchConceptsStable transcription complex formationTRNA genesTranscription complex formationStable transcription complexesTranscription complexDrosophila Kc cell extractGene regionD-stemDrosophila tRNAArg geneEukaryotic tRNA genesDrosophila tRNA genesTranscription termination sequenceTRNAArg geneStable complexesComplex formationTranscription experimentsDNA regionsTranscription factorsFactor bindingCell-free extractsTermination sequenceSequence 5T-stemCell extractsDeletion mutations