1990
The RNA component of RNase P in Schizosaccharomyces species
Zimmerly S, Gamulin V, Burkard U, Söll D. The RNA component of RNase P in Schizosaccharomyces species. FEBS Letters 1990, 271: 189-193. PMID: 2226803, DOI: 10.1016/0014-5793(90)80403-6.Peer-Reviewed Original ResearchConceptsSchizosaccharomyces speciesS. pombeS. octosporusFission yeast SchizosaccharomycesSecondary structure modelComparative structural informationYeast SchizosaccharomycesGenes divergeRNase PRelated organismsSingle geneRNA componentNorthern analysisK RNAGenesS. japonicusSpeciesPombeOctosporusRNAStructural informationSequenceSchizosaccharomycesCopurifiesCloning
1986
Functional complementation between mutations in a yeast suppressor tRNA gene reveals potential for evolution of tRNA sequences.
Willis I, Nichols M, Chisholm V, Söll D, Heyer W, Szankasi P, Amstutz H, Munz P, Kohli J. Functional complementation between mutations in a yeast suppressor tRNA gene reveals potential for evolution of tRNA sequences. Proceedings Of The National Academy Of Sciences Of The United States Of America 1986, 83: 7860-7864. PMID: 3532123, PMCID: PMC386822, DOI: 10.1073/pnas.83.20.7860.Peer-Reviewed Original ResearchConceptsMutant tRNA precursorS. pombe genesSuppressor tRNA geneNucleotide sequence evolutionRNA processing levelRNase P cleavagePombe geneTRNA genesFunctional complementationComplementation eventsS. pombeCycle of inactivationTRNA sequencesTRNA precursorsSequence evolutionSaccharomyces cerevisiaeS. cerevisiaePombe strainSchizosaccharomyces pombe strainStructural domainsDifferential expressionSuppressor functionP cleavageGenesSuppressor
1985
First identification of an amber nonsense mutation in Schizosaccharomyces pombe: major differences in the efficiency of homologous versus heterologous yeast suppressor tRNA genes
Krupp G, Thuriaux P, Willis I, Gamulin V, Söll D. First identification of an amber nonsense mutation in Schizosaccharomyces pombe: major differences in the efficiency of homologous versus heterologous yeast suppressor tRNA genes. Molecular Genetics And Genomics 1985, 201: 82-87. PMID: 3903436, DOI: 10.1007/bf00397990.Peer-Reviewed Original ResearchConceptsS. pombeAmber allelesAmber suppressor allelesFission yeast SchizosaccharomycesS. pombe transformantsAmber suppressor tRNANonsense mutationAmber nonsense mutationsSuppressor tRNA geneTRNA genesFission yeastYeast SchizosaccharomycesSchizosaccharomyces pombeSuppressor allelesTRP1 locusAmber mutationSuppressor tRNAPombeNonsense allelesNorthern analysisNitrosoguanidine mutagenesisOchre alleleGenesFirst identificationTRNASer
1984
The Schizosaccharomyces pombe sup3‐i suppressor recognizes ochre, but not amber codons in vitro and in vivo.
Hottinger H, Stadelmann B, Pearson D, Frendewey D, Kohli J, Söll D. The Schizosaccharomyces pombe sup3‐i suppressor recognizes ochre, but not amber codons in vitro and in vivo. The EMBO Journal 1984, 3: 423-428. PMID: 6370683, PMCID: PMC557361, DOI: 10.1002/j.1460-2075.1984.tb01823.x.Peer-Reviewed Original ResearchConceptsFission yeast Schizosaccharomyces pombeYeast Schizosaccharomyces pombeUGA termination codonVitro translation assaysReadthrough productS. pombeSchizosaccharomyces pombeNonsense mutantsTermination signalOchre suppressorUGA suppressionTranslation assaysAmber codonTermination codonGlobin mRNASup3PombeT substitutionCodonSuppressorPlasmid DNASchizosaccharomycesMutantsVivoAnticodon
1982
The 5S RNA genes of Schizosaccharomyces pombe
Mao J, Appel B, Schaack J, Sharp S, Yamada H, Söll D. The 5S RNA genes of Schizosaccharomyces pombe. Nucleic Acids Research 1982, 10: 487-500. PMID: 6278416, PMCID: PMC326152, DOI: 10.1093/nar/10.2.487.Peer-Reviewed Original Research
1980
Dimeric transfer RNA precursors in S. pombe
Mao J, Schmidt O, Söll D. Dimeric transfer RNA precursors in S. pombe. Cell 1980, 21: 509-516. PMID: 7407924, DOI: 10.1016/0092-8674(80)90488-2.Peer-Reviewed Original ResearchConceptsTRNA genesNucleotide intronRNA precursorsDNA fragmentsNuclear extractsNucleotide spacerPrimary transcription productTransfer RNA precursorsNucleotide leader sequenceS. pombeTRNA sequencesTrailer sequencesRNA initiationLeader sequenceTRNA moleculesTranscription productsEnzymatic stepsSequence analysisRNA analysisXenopus oocytesGenesEndonuclease cleavageIntronsTerminusSequence
1979
Characterization of a UGA-suppressing serine tRNA from Schizosaccharomyces pombe with the help of a new in vitro assay system for eukaryotic suppressor tRNAs.
Kohli J, Kwong T, Altruda F, Söll D, Wahl G. Characterization of a UGA-suppressing serine tRNA from Schizosaccharomyces pombe with the help of a new in vitro assay system for eukaryotic suppressor tRNAs. Journal Of Biological Chemistry 1979, 254: 1546-1551. PMID: 762155, DOI: 10.1016/s0021-9258(17)37806-7.Peer-Reviewed Original ResearchConceptsOpal suppressor tRNASuppressor tRNAReadthrough productSchizosaccharomyces pombeFission yeast Schizosaccharomyces pombeYeast Schizosaccharomyces pombeOchre suppressor tRNAUGA termination codonBeta-globin mRNARabbit beta-globin mRNARabbit globin mRNAWheat germ extractSuppressor mutantsS. pombeNonsense suppressionPure tRNAsAlpha-globinSerine tRNATermination codonGlobin mRNATRNAPombeGerm extractSerineAssay system