2022
Uncovering translation roadblocks during the development of a synthetic tRNA
Prabhakar A, Krahn N, Zhang J, Vargas-Rodriguez O, Krupkin M, Fu Z, Acosta-Reyes FJ, Ge X, Choi J, Crnković A, Ehrenberg M, Puglisi EV, Söll D, Puglisi J. Uncovering translation roadblocks during the development of a synthetic tRNA. Nucleic Acids Research 2022, 50: 10201-10211. PMID: 35882385, PMCID: PMC9561287, DOI: 10.1093/nar/gkac576.Peer-Reviewed Original ResearchConceptsOrthogonal translation systemGenetic code expansionCode expansionTertiary interactionsNon-canonical amino acidsAminoacyl-tRNA substratesDomains of lifeAminoacyl-tRNA synthetaseTranslation systemSingle nucleotide mutationsSingle-molecule fluorescenceDistinct tRNAsNon-canonical structuresSelenocysteine insertionRibosomal translationTRNARibosomesSynthetic tRNANucleotide mutationsAmino acidsSame organismP siteOrganismsTranslocationTranslation
2013
Back Cover: Rewiring Translation for Elongation Factor Tu‐Dependent Selenocysteine Incorporation (Angew. Chem. Int. Ed. 5/2013)
Aldag C, Bröcker M, Hohn M, Prat L, Hammond G, Plummer A, Söll D. Back Cover: Rewiring Translation for Elongation Factor Tu‐Dependent Selenocysteine Incorporation (Angew. Chem. Int. Ed. 5/2013). Angewandte Chemie International Edition 2013, 52: 1596-1596. DOI: 10.1002/anie.201300063.Peer-Reviewed Original Research
2004
Cys-tRNACys formation and cysteine biosynthesis in methanogenic archaea: two faces of the same problem?
Ambrogelly A, Kamtekar S, Sauerwald A, Ruan B, Tumbula-Hansen D, Kennedy D, Ahel I, Söll D. Cys-tRNACys formation and cysteine biosynthesis in methanogenic archaea: two faces of the same problem? Cellular And Molecular Life Sciences 2004, 61: 2437-2445. PMID: 15526152, DOI: 10.1007/s00018-004-4194-9.Peer-Reviewed Original ResearchConceptsMethanogenic archaeaCysteine biosynthesisCellular translation machineryAminoacyl-tRNA synthesisCanonical cysteinyl-tRNA synthetaseAminoacyl-tRNA synthetasesCysteinyl-tRNA synthetaseRecognizable genesTranslation machineryGenome sequenceArchaeaBiosynthesisEssential componentSynthetasesTRNARibosomesGenesMachineryOrganismsSynthetasePossible linkSequenceFormation
1983
Organization and Expression of tRNA Genes in Drosophila Melanogaster
Sharp S, Cooley L, DeFranco D, Dingermann T, Söll D. Organization and Expression of tRNA Genes in Drosophila Melanogaster. Recent Results In Cancer Research 1983, 84: 1-14. PMID: 6405456, DOI: 10.1007/978-3-642-81947-6_1.Peer-Reviewed Original ResearchConceptsProtein-synthesizing machineryCorrect amino acidCognate aminoacyl-tRNA synthetasesAminoacyl-tRNA synthetasesTransfer RNA moleculesSpecific recognition featuresStructure-function relationshipsTRNA genesDrosophila melanogasterTRNA speciesRNA moleculesGeneral structural featuresPolypeptide chainTRNAAmino acidsRegulatory processesUseful moleculesFunctional characteristicsStructural featuresIntegral componentRecognition featuresMelanogasterEfficient recognitionSynthetasesRibosomes
1968
Studies on polynucleotides LXXXV. Partial purification of an amber supressor tRNA and studies on in vitro suppression
Söll D. Studies on polynucleotides LXXXV. Partial purification of an amber supressor tRNA and studies on in vitro suppression. Journal Of Molecular Biology 1968, 34: 175-187. PMID: 4938541, DOI: 10.1016/0022-2836(68)90243-x.Peer-Reviewed Original ResearchConceptsSuppressor tRNAColi cellsAmber suppressor genesAmber suppressor tRNAProtein synthesis experimentsEscherichia coli cellsE. coli cellsAmber mutantsTRNASuppressor geneProtein synthesisCrude tRNAGenesRNAPartial purificationBacteriophage f2CellsMutantsRibosomesUAGSpeciesMessengerSuppressionChain terminationBinding
1967
Studies on polynucleotides LXXV. Specificity of tRNA for codon recognition as studied by the ribosomal binding technique
Söll D, Cherayil J, Bock R. Studies on polynucleotides LXXV. Specificity of tRNA for codon recognition as studied by the ribosomal binding technique. Journal Of Molecular Biology 1967, 29: 97-112. PMID: 4861614, DOI: 10.1016/0022-2836(67)90183-0.Peer-Reviewed Original ResearchConceptsTransfer RNAAmino acidsE. coliIndividual amino acidsCodon recognitionMultiple codonsMultiple speciesRespective amino acidsWobble hypothesisYeast transfer RNAEscherichia coliCodonRNAColiYeastSpeciesBindingRecognition patternsTRNARibosomesThird letterStimulation of bindingTrinucleotideAcidInteresting differences
1966
Specificity of sRNA for recognition of codons as studied by the ribosomal binding technique
Söll D, Jones D, Ohtsuka E, Faulkner R, Lohrmann R, Hayatsu H, Khorana H, Cherayil J, Hampel A, Bock R. Specificity of sRNA for recognition of codons as studied by the ribosomal binding technique. Journal Of Molecular Biology 1966, 19: 556-573. PMID: 5338858, DOI: 10.1016/s0022-2836(66)80023-2.Peer-Reviewed Original Research