1989
A selection for mutants of the RNA polymerase III transcription apparatus: PCF1 stimulates transcription of tRNA and 5S RNA genes.
Willis I, Schmidt P, Söll D. A selection for mutants of the RNA polymerase III transcription apparatus: PCF1 stimulates transcription of tRNA and 5S RNA genes. The EMBO Journal 1989, 8: 4281-4288. PMID: 2686985, PMCID: PMC401634, DOI: 10.1002/j.1460-2075.1989.tb08614.x.Peer-Reviewed Original ResearchMeSH KeywordsBase SequenceCloning, MolecularDNA-Directed RNA PolymerasesGene ExpressionGenes, DominantGenes, FungalKineticsMolecular Sequence DataMutationOligonucleotide ProbesPlasmidsPromoter Regions, GeneticRNA Polymerase IIIRNA, RibosomalRNA, Ribosomal, 5SRNA, TransferSaccharomyces cerevisiaeSaccharomycetalesSchizosaccharomycesSelection, GeneticSuppression, GeneticTemplates, GeneticTranscription, GeneticConceptsTRNA genesMutant strainTranscription of mutantsTranscription of tRNARNA polymerase IIISuppressor tRNA geneDominant mutant geneWild-type strainStable complexesTranscription apparatusRNA genesStable complex formationUpstream geneTRNA suppressorsPositive regulatorSteady-state levelsComplex assemblyGenetic approachesPolymerase IIIGene transcriptionInternal promoterMutant geneTime-course experimentsTranscriptionGenes
1986
Inactivation of nonsense suppressor transfer RNA genes in Schizosaccharomyces pombe Intergenic conversion and hot spots of mutation
Heyer W, Münz P, Amstutz H, Aebi R, Gysler C, Schuchert P, Szankasi P, Leupold U, Kohli J, Gamulin V, Söll D. Inactivation of nonsense suppressor transfer RNA genes in Schizosaccharomyces pombe Intergenic conversion and hot spots of mutation. Journal Of Molecular Biology 1986, 188: 343-353. PMID: 3735426, DOI: 10.1016/0022-2836(86)90159-2.Peer-Reviewed Original ResearchConceptsTRNA genesSuppressor tRNA geneIntergenic conversionDNA sequencesTransfer RNA genesYeast Schizosaccharomyces pombeSerine tRNA geneCrosses of strainsSame molecular mechanismsConcerted evolutionRNA genesProgeny sporesSchizosaccharomyces pombeAllelic conversionDifferent chromosomesConversion eventsIntron sequencesSequence transferMolecular mechanismsMutation hot spotsSpontaneous mutationsVegetative cellsGenesPoint mutationsSuppressor activity
1984
Transcriptionally active and inactive gene repeats within the D. meianogaster 5S RNA gene cluster
Sharp S, Garcia A, Cooley L, Söll D. Transcriptionally active and inactive gene repeats within the D. meianogaster 5S RNA gene cluster. Nucleic Acids Research 1984, 12: 7617-7632. PMID: 6093044, PMCID: PMC320189, DOI: 10.1093/nar/12.20.7617.Peer-Reviewed Original ResearchConceptsEfficiency of transcriptionRRNA gene copiesHigh transcription efficiencyTwo-nucleotide deletionD. melanogasterGene repeatRNA genesGene clusterPrimary transcriptGene copiesTranscription functionTranscription efficiencyTemplate activityCell extractsTranscriptionDNAPosition 28Position 86DNA typesRepeat unitsDeletionSame sequenceMelanogasterRRNAGenes
1983
Partial purification of Drosophila Kc cell RNA polymerase III transcription components. Evidence for shared 5 S RNA and tRNA gene factors.
Burke D, Schaack J, Sharp S, Söll D. Partial purification of Drosophila Kc cell RNA polymerase III transcription components. Evidence for shared 5 S RNA and tRNA gene factors. Journal Of Biological Chemistry 1983, 258: 15224-15231. PMID: 6197413, DOI: 10.1016/s0021-9258(17)43797-5.Peer-Reviewed Original ResearchConceptsS RNA geneTranscription componentsRNA polymerase IIIRNA genesPolymerase IIIStable transcription complex formationReconstitution of transcriptionTranscription complex formationDrosophila Kc cellsCM-Sepharose column chromatographyDrosophila tRNAKc cellsTranscription factorsS RNATRNAGene factorsCompetition experimentsTranscriptionGenesComplex formationPartial purificationDEAE-SephadexReconstitutionCofractionationColumn chromatographyOrganization of ribosomal DNA in yellow lupine (Lupinus luteus) and sequence of the 5.8 S RNA gene
Rafalski J, Wiewiórowski M, Söll D. Organization of ribosomal DNA in yellow lupine (Lupinus luteus) and sequence of the 5.8 S RNA gene. FEBS Letters 1983, 152: 241-246. DOI: 10.1016/0014-5793(83)80388-3.Peer-Reviewed Original Research
1982
The 5.8S RNA gene sequence and the ribosomal repeat of Schizosaccharomyces pombe
Schaak J, Mao J, Söll D. The 5.8S RNA gene sequence and the ribosomal repeat of Schizosaccharomyces pombe. Nucleic Acids Research 1982, 10: 2851-2864. PMID: 6285312, PMCID: PMC320660, DOI: 10.1093/nar/10.9.2851.Peer-Reviewed Original ResearchConceptsRNA genesSchizosaccharomyces pombeRNA speciesGene sequencesDNA fragmentsKb HindIII DNA fragmentRRNA gene repeatsRNA gene sequencesKb DNA fragmentHindIII DNA fragmentSecondary structure modelNumber of nucleotidesHigher eukaryotesGene repeatIntergenic spacerN. crassaRibosomal repeatNucleotide sequenceS. cerevisiaeGreatest homologySequence differencesVariable stemGenesRNARepeatsThe 5S RNA genes of Schizosaccharomyces pombe
Mao J, Appel B, Schaack J, Sharp S, Yamada H, Söll D. The 5S RNA genes of Schizosaccharomyces pombe. Nucleic Acids Research 1982, 10: 487-500. PMID: 6278416, PMCID: PMC326152, DOI: 10.1093/nar/10.2.487.Peer-Reviewed Original Research
1981
Transcription of cloned tRNA and 5S RNA genes in a Drosophila cell free extract
Dingermann T, Sharp S, Appel B, DeFranco D, Mount S, Heiermann R, Pongs O, Söll D. Transcription of cloned tRNA and 5S RNA genes in a Drosophila cell free extract. Nucleic Acids Research 1981, 9: 3907-3918. PMID: 6170932, PMCID: PMC327404, DOI: 10.1093/nar/9.16.3907.Peer-Reviewed Original Research