1985
Leucine tRNA family of Escherichia coli: nucleotide sequence of the supP(Am) suppressor gene
Thorbjarnardóttir S, Dingermann T, Rafnar T, Andrésson O, Söll D, Eggertsson G. Leucine tRNA family of Escherichia coli: nucleotide sequence of the supP(Am) suppressor gene. Journal Of Bacteriology 1985, 161: 219-222. PMID: 2981802, PMCID: PMC214859, DOI: 10.1128/jb.161.1.219-222.1985.Peer-Reviewed Original ResearchConceptsSuppressor allelesLeuX geneAmber suppressor allelesMature coding sequenceLeucyl-tRNA synthetaseSingle base changeTRNA familiesCAA anticodonBox sequenceTermination signalDNA sequencesNucleotide sequenceBacteriophage T4Coding sequenceAminoacyl stemSuppressor geneLoop regionTRNABase changesEscherichia coliGenesE. coliSequenceColiAlleles
1979
Regulation of biosynthesis of aminoacyl-transfer RNA synthetases and of transfer-RNA in Escherichia coli.
Morgan S, Larossa R, Cheung A, Low B, Söll D. Regulation of biosynthesis of aminoacyl-transfer RNA synthetases and of transfer-RNA in Escherichia coli. Biological Research 1979, 12: 415-26. PMID: 45219.Peer-Reviewed Original ResearchConceptsGlutaminyl-tRNA synthetaseLeucyl-tRNA synthetaseGlnR mutantRegulation of biosynthesisClasses of mutantsTemperature-resistant revertantsOperator-promoter regionAminoacyl-transfer RNA synthetasesStructural geneLeu genesRegulatory mutantsThermolabile leucyl-tRNA synthetaseMutantsResistant revertantsFormation of glutamineGlutamine synthetaseE. coli strainsEscherichia coliRNA synthetasesGenesSynthetaseElevated levelsRevertantsColi strainsGlnT
1977
Regulation of biosynthesis of aminoacyl-tRNA synthetases and of tRNA in Escherichia coli II. Isolation of regulatory mutants affecting leucyl-tRNA synthetase levels
LaRossa R, Vögeli G, Low K, Söll D. Regulation of biosynthesis of aminoacyl-tRNA synthetases and of tRNA in Escherichia coli II. Isolation of regulatory mutants affecting leucyl-tRNA synthetase levels. Journal Of Molecular Biology 1977, 117: 1033-1048. PMID: 342703, DOI: 10.1016/s0022-2836(77)80011-9.Peer-Reviewed Original ResearchConceptsLeucyl-tRNA synthetaseThermolabile leucyl-tRNA synthetaseRegulation of biosynthesisDistinct genetic lociAminoacyl-tRNA synthetasesEscherichia coli IIOperator-promoter regionStructural geneRegulatory mutantsGenetic lociEscherichia coli strainsSuch revertantsSpontaneous revertantsThermolabile enzymeLociSynthetaseColi strainsRevertantsSynthetase levelsLeuTRNAMutantsSynthetasesLeuXBiosynthesisRegulation of biosynthesis of aminoacyl-tRNA synthetases and of tRNA in Escherichia coli III. Biochemical characterization of regulatory mutants affecting leucyl-tRNA synthetase levels
LaRossa R, Mao J, Low K, Söll D. Regulation of biosynthesis of aminoacyl-tRNA synthetases and of tRNA in Escherichia coli III. Biochemical characterization of regulatory mutants affecting leucyl-tRNA synthetase levels. Journal Of Molecular Biology 1977, 117: 1049-1059. PMID: 342704, DOI: 10.1016/s0022-2836(77)80012-0.Peer-Reviewed Original ResearchConceptsAminoacyl-tRNA synthetasesLeucyl-tRNA synthetaseProtein synthesisRegulation of biosynthesisEscherichia coli IIIIsoacceptor familiesIlv operonRegulatory mutantsSteady-state levelsAmount of tRNALeu operonBiochemical characterizationCellular concentrationSynthetasesEscherichia coliOperonControl elementsTRNASynthetaseSynthetase levelsMutationsDerepressionPpGppMutantsPppGpp
1971
Isolation and Partial Characterization of Temperature-Sensitive Escherichia coli Mutants with Altered Leucyl- and Seryl-Transfer Ribonucleic Acid Synthetases
Low B, Gates F, Goldstein T, Söll D. Isolation and Partial Characterization of Temperature-Sensitive Escherichia coli Mutants with Altered Leucyl- and Seryl-Transfer Ribonucleic Acid Synthetases. Journal Of Bacteriology 1971, 108: 742-750. PMID: 4942762, PMCID: PMC247134, DOI: 10.1128/jb.108.2.742-750.1971.Peer-Reviewed Original ResearchConceptsLeucyl-tRNA synthetaseTemperature-sensitive Escherichia coli mutantsCorresponding genetic lociEscherichia coli mutantsSeryl-tRNA synthetaseTemperature-sensitive mutantColi mutantsGenetic lociBranched-chain amino acidsEscherichia coliAmino acidsConditional growthSynthetaseMutantsPartial characterizationEnzymePurification of Five Leucine Transfer Ribonucleic Acid Species from Escherichia coli and Their Acylation by Heterologous Leucyl-Transfer Ribonucleic Acid Synthetase
Blank H, Söll D. Purification of Five Leucine Transfer Ribonucleic Acid Species from Escherichia coli and Their Acylation by Heterologous Leucyl-Transfer Ribonucleic Acid Synthetase. Journal Of Biological Chemistry 1971, 246: 4947-4950. PMID: 4936719, DOI: 10.1016/s0021-9258(18)61954-4.Peer-Reviewed Original ResearchMeSH KeywordsAcylationBase SequenceBenzoatesCarbon IsotopesCarcinomaCell LineChromatography, DEAE-CelluloseChromatography, GelDrug StabilityEscherichia coliGenetic CodeHot TemperatureKineticsLeucineLigasesMouth NeoplasmsNucleic Acid DenaturationPolynucleotidesRibosomesRNA, BacterialRNA, TransferTemplates, GeneticYeastsA Comparative Study of the Interactions of Escherichia coli Leucyl-, Seryl-, and Valyl-Transfer Ribonucleic Acid Synthetases with Their Cognate Transfer Ribonucleic Acids
Myers G, Blank H, Söll D. A Comparative Study of the Interactions of Escherichia coli Leucyl-, Seryl-, and Valyl-Transfer Ribonucleic Acid Synthetases with Their Cognate Transfer Ribonucleic Acids. Journal Of Biological Chemistry 1971, 246: 4955-4964. PMID: 4936720, DOI: 10.1016/s0021-9258(18)61956-8.Peer-Reviewed Original ResearchConceptsEscherichia coli KSeryl-tRNA synthetaseLeucyl-tRNA synthetaseRibonucleic acidTransfer ribonucleic acidValyl-tRNA synthetaseTRNA recognitionColi KSynthetase-tRNA complexIsoacceptorsAmino acidsEquilibrium binding studiesPing-pong typeTRNASynthetaseEnzymeKm valuesSubstrate inhibitionBasic similaritiesBinding studiesSerylAcidATPSame bufferSequencePurification of an Escherichia coli Leucine Suppressor Transfer Ribonucleic Acid and Its Aminoacylation by the Homologous Leucyl-Transfer Ribonucleic Acid Synthetase
Hayashi H, Söll D. Purification of an Escherichia coli Leucine Suppressor Transfer Ribonucleic Acid and Its Aminoacylation by the Homologous Leucyl-Transfer Ribonucleic Acid Synthetase. Journal Of Biological Chemistry 1971, 246: 4951-4954. PMID: 4941862, DOI: 10.1016/s0021-9258(18)61955-6.Peer-Reviewed Original ResearchMeSH KeywordsAcylationBenzoatesBiological AssayCarbon IsotopesChromatography, DEAE-CelluloseColiphagesEscherichia coliGenetics, MicrobialKineticsLeucineLigasesMutationPeptide BiosynthesisPlant Growth RegulatorsPlants, ToxicPolynucleotidesRNA, TransferSuppression, GeneticTemplates, GeneticTobaccoValine
1970
The Interaction of Seryl and of Leucyl Transfer Ribonucleic Acid Synthetases with Their Cognate Transfer Ribonucleic Acids
Knowles J, Katze J, Konigsberg W, Söll D. The Interaction of Seryl and of Leucyl Transfer Ribonucleic Acid Synthetases with Their Cognate Transfer Ribonucleic Acids. Journal Of Biological Chemistry 1970, 245: 1407-1415. PMID: 4910800, DOI: 10.1016/s0021-9258(18)63251-x.Peer-Reviewed Original ResearchConceptsSeryl-tRNA synthetaseTransfer ribonucleic acidComplex formationTransfer RNA speciesLeucyl-tRNA synthetaseRibonucleic acidRNA speciesCognate tRNAEscherichia coliSynthetaseDensity gradient centrifugationTRNAStable complexesHigh saltGradient centrifugationSpeciesGel filtrationComplexesSerylColiATPEnzymeAcidSerFormation