2023
Split aminoacyl-tRNA synthetases for proximity-induced stop codon suppression
Jiang H, Ambrose N, Chung C, Wang Y, Söll D, Tharp J. Split aminoacyl-tRNA synthetases for proximity-induced stop codon suppression. Proceedings Of The National Academy Of Sciences Of The United States Of America 2023, 120: e2219758120. PMID: 36787361, PMCID: PMC9974479, DOI: 10.1073/pnas.2219758120.Peer-Reviewed Original ResearchConceptsAminoacyl-tRNA synthetasesCodon suppressionStop codon suppressionGene expressionOrthogonal aminoacyl-tRNA synthetasesRelevant protein-protein interactionsSynthetic biology toolsSmall molecule rapamycinControl gene expressionProtein-protein interactionsLevel of transcriptionAbscisic acidDimerization domainMammalian cellsBiology toolsGene translationTranslational levelMolecular switchStop codonHuman cellsMolecular inputsUseful biotechnologySynthetasesExpressionTherapeutic applications
2000
Antisense HEMA1 RNA Expression Inhibits Heme and Chlorophyll Biosynthesis in Arabidopsis1
Kumar A, Söll D. Antisense HEMA1 RNA Expression Inhibits Heme and Chlorophyll Biosynthesis in Arabidopsis1. Plant Physiology 2000, 122: 49-56. PMID: 10631248, PMCID: PMC58843, DOI: 10.1104/pp.122.1.49.Peer-Reviewed Original ResearchConceptsConstitutive cauliflower mosaic virus 35S promoterCauliflower mosaic virus 35S promoterTransgenic Arabidopsis plantsGlutamyl-tRNA reductaseBiosynthesis of tetrapyrrolesNormal growth conditionsLevels of chlorophyllFormation of ALAArabidopsis plantsChlorophyll biosynthesisHemA geneChlorophyll deficiencyGsa geneFirst enzymeGene expressionEnzymatic stepsSecond enzymeHeme synthesisPlantsReductase expressionChlorophyllGrowth conditionsBiosynthesisHemeGenes
1999
Selective inhibition of HEMA gene expression by photooxidation in Arabidopsis thaliana
Kumar M, Chaturvedi S, Söll D. Selective inhibition of HEMA gene expression by photooxidation in Arabidopsis thaliana. Phytochemistry 1999, 51: 847-851. PMID: 10423858, DOI: 10.1016/s0031-9422(99)00114-4.Peer-Reviewed Original ResearchConceptsArabidopsis thalianaChloroplasts of plantsGlutamyl-tRNA reductaseCarotenoid biosynthesisFirst enzymeALA formationPhotobleaching herbicidesPhotooxidative damageGene expressionSelective inhibitionCarotenoid pigmentsNorflurazonThalianaPlantsChloroplastsFirst precursorPathwayExpressionEnzymeInitial metaboliteAlaBiosynthesisInhibitionTetrapyrrolesGlutamateArchaeal Aminoacyl-tRNA Synthesis: Diversity Replaces Dogma
Tumbula D, Vothknecht U, Kim H, Ibba M, Min B, Li T, Pelaschier J, Stathopoulos C, Becker H, Söll D. Archaeal Aminoacyl-tRNA Synthesis: Diversity Replaces Dogma. Genetics 1999, 152: 1269-1276. PMID: 10430557, PMCID: PMC1460689, DOI: 10.1093/genetics/152.4.1269.Peer-Reviewed Original ResearchConceptsAminoacyl-tRNA synthesisGene transfer eventsPhenylalanyl-tRNA synthetasesLysyl-tRNA synthetaseTransamidation pathwayExtant organismsMethanococcus jannaschiiAsparaginyl-tRNAProtein biosynthesisGenetic codeGene expressionGenome sequencingAminoacyl-tRNAArchaeaMethanobacterium thermoautotrophicumMolecular biologyUnexpected levelNovel pathwayTransfer eventsFaithful translationPathwayJannaschiiSynthetasesBiosynthesisOrganisms
1992
Organization and nucleotide sequence of the glutamine synthetase (glnA) gene from Lactobacillus delbrueckii subsp. bulgaricus
Ishino Y, Morgenthaler P, Hottinger H, Söll D. Organization and nucleotide sequence of the glutamine synthetase (glnA) gene from Lactobacillus delbrueckii subsp. bulgaricus. Applied And Environmental Microbiology 1992, 58: 3165-3169. PMID: 1359838, PMCID: PMC183065, DOI: 10.1128/aem.58.9.3165-3169.1992.Peer-Reviewed Original ResearchConceptsOpen reading frameGlnA geneReading frameGlnA gene expressionGlutamine synthetase geneL. delbrueckii subspDNA sequence analysisMaxicell methodLactobacillus delbrueckii subspDelbrueckii subspGlnR geneDeletion strainNegative eubacteriaPositive eubacteriaSynthetase geneExtensive homologyRepressor geneNucleotide sequencePresent upstreamGene expressionBamHI fragmentUnknown functionSequence analysisClostridium acetobutylicumGenes
1981
Gene Expression- The Production of RNA's Cell Biology: A Comprehensive Treatise, Volume 3. Lester Goldstein , David M. Prescott
Soll D. Gene Expression- The Production of RNA's Cell Biology: A Comprehensive Treatise, Volume 3. Lester Goldstein , David M. Prescott. The Quarterly Review Of Biology 1981, 56: 333-334. DOI: 10.1086/412354.Commentaries, Editorials and Letters
1979
Suppression
Steege D, Söll D. Suppression. Biological Regulation And Development 1979, 433-485. DOI: 10.1007/978-1-4684-3417-0_11.Peer-Reviewed Original ResearchGenetic suppressionType phenotypeFinal gene productsWild-type phenotypePairs of genesInformational suppressorsSuppressor mutationsNonsense suppressionMissense suppressionGene productsMolecular basisFrameshift suppressionGene expressionMutant organismsMolecular mechanismsCell metabolismGenetic selectionGenetic termsMutationsSecondary mutationsTranscriptionMacromolecular componentsPrimary mutationsSuppressorPhenotype