2013
PI3Kγ inhibition alleviates symptoms and increases axon number in experimental autoimmune encephalomyelitis mice
Li H, Park D, Abdul-Muneer P, Xu B, Wang H, Xing B, Wu D, Li S. PI3Kγ inhibition alleviates symptoms and increases axon number in experimental autoimmune encephalomyelitis mice. Neuroscience 2013, 253: 89-99. PMID: 24012746, PMCID: PMC9529370, DOI: 10.1016/j.neuroscience.2013.08.051.Peer-Reviewed Original ResearchMeSH KeywordsAnimalsAxonsCD3 ComplexClass Ib Phosphatidylinositol 3-KinaseDioxolesDisease Models, AnimalEctodysplasinsEncephalomyelitis, Autoimmune, ExperimentalEnzyme InhibitorsGene Expression RegulationMiceMice, Inbred C57BLMice, KnockoutMyelin SheathMyelin-Oligodendrocyte GlycoproteinNeurofilament ProteinsPeptide FragmentsPhosphoinositide-3 Kinase InhibitorsSerotoninSeverity of Illness IndexSpinal CordThiazolidinedionesTime FactorsConceptsExperimental autoimmune encephalomyelitisMultiple sclerosisEAE miceSpinal cordExperimental autoimmune encephalomyelitis (EAE) miceAutoimmune CNS inflammationLumbar spinal cordNumber of axonsCNS inflammationAutoimmune encephalomyelitisSystemic treatmentClinical symptomsPI3Kγ inhibitionInflammatory cellsAxon numberClinical signsInflammatory responseInflammatory reactionKnockout miceFiber tractsMicePI3Kγ inhibitorsCordSymptomsPI3Kγ
2002
Regulation of Gli1 Transcriptional Activity in the Nucleus by Dyrk1*
Mao J, Maye P, Kogerman P, Tejedor F, Toftgard R, Xie W, Wu G, Wu D. Regulation of Gli1 Transcriptional Activity in the Nucleus by Dyrk1*. Journal Of Biological Chemistry 2002, 277: 35156-35161. PMID: 12138125, DOI: 10.1074/jbc.m206743200.Peer-Reviewed Original ResearchMeSH Keywords3T3 CellsAnimalsCell DifferentiationCell NucleusCOS CellsGene Expression RegulationHedgehog ProteinsMiceOncogene ProteinsPhosphorylationPrecipitin TestsProtein Serine-Threonine KinasesProtein TransportProtein-Tyrosine KinasesSignal TransductionTrans-ActivatorsTranscription FactorsTranscription, GeneticZinc Finger Protein GLI1ConceptsTranscriptional activityGene transcriptionDual-specificity protein kinaseNuclear export mutantGLI1 transcriptional activityMouse C3H10T1/2 cellsReporter gene assayCellular rolesExport mutantsTranscriptional regulationProtein kinaseKinase activityC3H10T1/2 cellsLEF-1Kinase 1DYRK1Sonic hedgehogC-JunGene assayCell nucleiShhTranscriptionRegulationGli1Pathway
1999
Genetic alteration of phospholipase C β3 expression modulates behavioral and cellular responses to μ opioids
Xie W, Samoriski G, McLaughlin J, Romoser V, Smrcka A, Hinkle P, Bidlack J, Gross R, Jiang H, Wu D. Genetic alteration of phospholipase C β3 expression modulates behavioral and cellular responses to μ opioids. Proceedings Of The National Academy Of Sciences Of The United States Of America 1999, 96: 10385-10390. PMID: 10468617, PMCID: PMC17897, DOI: 10.1073/pnas.96.18.10385.Peer-Reviewed Original ResearchMeSH KeywordsAnimalsBrainCalcium ChannelsCell MembraneEnkephalin, Ala(2)-MePhe(4)-Gly(5)-EnkephalinsGanglia, SpinalGene Expression RegulationGene Expression Regulation, EnzymologicIsoenzymesMembrane PotentialsMiceMice, KnockoutMorphineNeurons, AfferentPainPhospholipase C betaReceptors, Opioid, deltaReceptors, Opioid, kappaReceptors, Opioid, muType C PhospholipasesConceptsBeta3-null miceDorsal root ganglion neuronsPLC-beta3Mu-opioid responseOpioid receptor numberSpecific mu agonistPrimary sensory neuronsPhospholipase CDelta-opioid receptorsWild-type neuronsBeta3-deficient miceProtein kinase CDAMGO responseMicro opioidsOpioid responseOpioid regulationMu agonistsOpioid sensitivityGanglion neuronsΜ-opioidOpioid receptorsReceptor numberSensory neuronsKinase CNumber of intracellular
1998
Guanine nucleotide exchange factor GEF115 specifically mediates activation of Rho and serum response factor by the G protein α subunit Gα13
Mao J, Yuan H, Xie W, Wu D. Guanine nucleotide exchange factor GEF115 specifically mediates activation of Rho and serum response factor by the G protein α subunit Gα13. Proceedings Of The National Academy Of Sciences Of The United States Of America 1998, 95: 12973-12976. PMID: 9789025, PMCID: PMC23675, DOI: 10.1073/pnas.95.22.12973.Peer-Reviewed Original ResearchMeSH Keywords3T3 CellsAmino Acid SequenceAnimalsCOS CellsDNA-Binding ProteinsGene Expression RegulationGTP-Binding ProteinsGTPase-Activating ProteinsGuanine Nucleotide Exchange FactorsKineticsLuciferasesMiceMolecular Sequence DataNuclear ProteinsProteinsRecombinant Fusion ProteinsRhoA GTP-Binding ProteinSequence AlignmentSequence Homology, Amino AcidSerum Response FactorSignal TransductionTranscription FactorsTranscription, GeneticTransfectionConceptsSerum response factorActivation of RhoSRF activationHeterotrimeric G protein alpha subunitsG protein signaling (RGS) domainG protein alpha subunitsTerminal partRho-specific guanineProtein alpha subunitsResponse factorDomain deletion mutantSignal transduction pathwaysNIH 3T3 cellsDbl homologyRGS activitySequence motifsTransduction pathwaysGalpha13Signaling domainsGalpha12Alpha subunitMutantsSynergistic activationGalphaqTransfection systemSpecific Involvement of G Proteins in Regulation of Serum Response Factor-mediated Gene Transcription by Different Receptors*
Mao J, Yuan H, Xie W, Simon M, Wu D. Specific Involvement of G Proteins in Regulation of Serum Response Factor-mediated Gene Transcription by Different Receptors*. Journal Of Biological Chemistry 1998, 273: 27118-27123. PMID: 9765229, DOI: 10.1074/jbc.273.42.27118.Peer-Reviewed Original ResearchMeSH Keywords15-Hydroxy-11 alpha,9 alpha-(epoxymethano)prosta-5,13-dienoic Acid3T3 CellsAnimalsDNA-Binding ProteinsDrug SynergismEndothelinsGene Expression RegulationGTP-Binding ProteinsGTPase-Activating ProteinsLysophospholipidsMiceNuclear ProteinsProteinsReceptors, Cell SurfaceSerum Response FactorThrombinTranscription, GeneticConceptsThromboxane A2Lysophosphatidic acidType 1 muscarinic receptorG proteinsAlpha1-adrenergic receptorsInterleukin-8 receptorEndogenous G proteinsG protein-coupled receptorsProtein-coupled receptorsEndothelin receptorsMuscarinic receptorsDopamine receptorsSRF activationSerum response factorType 1Gene transcriptionReceptorsDifferent receptorsG protein subunitsSpecific involvementGalphaq/11Gq classCell linesNIH3T3 cellsThrombin