One-Step Modification of Electrode Surface for Ultrasensitive and Highly Selective Detection of Nucleic Acids with Practical Applications
Li C, Wu D, Hu X, Xiang Y, Shu Y, Li G. One-Step Modification of Electrode Surface for Ultrasensitive and Highly Selective Detection of Nucleic Acids with Practical Applications. Analytical Chemistry 2016, 88: 7583-7590. PMID: 27374134, DOI: 10.1021/acs.analchem.6b01250.Peer-Reviewed Original ResearchConceptsElectrode surfaceCapture probeAccessibility of target moleculesSingle-base discrimination abilityFunctionalized electrode surfaceTarget moleculesUnique probe designSignal probeHairpin DNAProtein repelling abilityComplex samplesRigid scaffoldsDetection of nucleic acidsOne-step modificationBinding modelElectrodeOne-stepAcid sensorsMoleculesNucleic acid sensorsProbe bindingNucleic acidsSurfaceMicro-RNAsPractical applicationsElectrochemical detection of DNA 3′-phosphatases based on surface-extended DNA nanotail strategy
Wu D, Li C, Hu X, Mao X, Li G. Electrochemical detection of DNA 3′-phosphatases based on surface-extended DNA nanotail strategy. Analytica Chimica Acta 2016, 924: 29-34. PMID: 27181641, DOI: 10.1016/j.aca.2016.04.013.Peer-Reviewed Original ResearchConceptsDNA 3'-phosphataseT4 polynucleotide kinase phosphatasePolynucleotide kinase phosphataseLow detection limitLong DNA strandsSubstrate DNACellular processesTerminal deoxynucleotidyl transferaseDNA probesDNA strandsGold electrodeDephosphorylation reactionsTerminal hydroxylElectrochemical detectionUltrasensitive detectionDetection limitDNAAnalytical performanceDeoxynucleotidyl transferaseSignal amplificationDrug discoveryDephosphorylationClinical diagnosticsSignal enhancementTdT