Biochemical and molecular characterization ofLeishmania pifanoi amastigotes in continuous axenic culture
Rainey P, Spithill T, McMahon-Pratt D, Pan A. Biochemical and molecular characterization ofLeishmania pifanoi amastigotes in continuous axenic culture. Molecular And Biochemical Parasitology 1991, 49: 111-118. PMID: 1775152, DOI: 10.1016/0166-6851(91)90134-r.Peer-Reviewed Original ResearchConceptsContinuous axenic cultureSynthesis of DNAMolecular geneticsAdenine deaminaseAmastigote-like formsAxenic cultureAxenic mediumExcellent modelAxenic formsLeishmania pifanoiMetabolism of glucosePeroxidase activityAmastigote formsPromastigote formsAmastigotesCulture formsGenesGeneticsRNAProteinDNAReductaseRibonucleaseLinoleic acidPrevious demonstrationBiochemical characterization of the protective membrane glycoprotein GP46/M-2 of Leishmania amazonensis
Rivas L, Kahl L, Manson K, McMahon-Pratt D. Biochemical characterization of the protective membrane glycoprotein GP46/M-2 of Leishmania amazonensis. Molecular And Biochemical Parasitology 1991, 47: 235-243. PMID: 1719417, DOI: 10.1016/0166-6851(91)90183-7.Peer-Reviewed Original ResearchConceptsGP46/MCarbohydrate side chainsDNA-derived protein sequenceLipid anchorSpecies-specific epitopesProtein sequencesSide chainsBiochemical characterizationLeishmania amazonensisProteinBiochemical featuresPolypeptide portionSurface membraneMonoclonal antibodiesPolypeptideAmazonensisMoleculesEpitopesAnchorsSequenceMembraneDigestionAntibodiesChainPromastigotes