1996
Leishmania amazonensis:Cultivation and Characterization of Axenic Amastigote-like Organisms
Hodgkinson V, Soong L, Duboise S, McMahon-Pratt D. Leishmania amazonensis:Cultivation and Characterization of Axenic Amastigote-like Organisms. Experimental Parasitology 1996, 83: 94-105. PMID: 8654556, DOI: 10.1006/expr.1996.0053.Peer-Reviewed Original ResearchConceptsAmastigote-like formsNorthern blot analysisDevelopmental biologyContinual cultureAxenic cultureMolecular biologyTransformation of promastigotesOrganismsDNA probesGeneration timeCultured amastigotesAxenic cultivationBlot analysisExpression levelsSpecies of LeishmaniaSpeciesProtein reactiveBiologyDegrees CFirst reportPeriod of growthAmastigotesPromastigotesLeishmania amazonensisCultivation
1989
Amastigote and epimastigote stage-specific components of Trypanosoma cruzi characterized by using monoclonal antibodies. Purification and molecular characterization of an 83-kilodalton amastigote protein.
Pan A, McMahon-Pratt D. Amastigote and epimastigote stage-specific components of Trypanosoma cruzi characterized by using monoclonal antibodies. Purification and molecular characterization of an 83-kilodalton amastigote protein. The Journal Of Immunology 1989, 143: 1001-8. PMID: 2501384, DOI: 10.4049/jimmunol.143.3.1001.Peer-Reviewed Original ResearchConceptsTissue culture-derived trypomastigotesTrypanosoma cruziCruzi amastigotesPreliminary sequence dataStage-specific componentsCulture-derived trypomastigotesStage-specific monoclonal antibodiesSingle protein moleculesMembrane-enriched preparationsAmino acid compositionSequence dataEpimastigote membranesHuman chagasic seraStage differentiationAxenic cultureMolecular characterizationT. cruzi amastigotesEpimastigotesProtein moleculesProteinChagas diseaseMonoclonal antibodiesLeishmania speciesAcid compositionChagasic sera
1988
Membrane glycoprotein M-2 protects against Leishmania amazonensis infection
Champsi J, McMahon-Pratt D. Membrane glycoprotein M-2 protects against Leishmania amazonensis infection. Infection And Immunity 1988, 56: 3272-3279. PMID: 3182080, PMCID: PMC259734, DOI: 10.1128/iai.56.12.3272-3279.1988.Peer-Reviewed Original ResearchConceptsMouse strainsComplete protectionBALB/c strainLeishmania amazonensis infectionT cell immunityProtective immune responseOnset of infectionC. parvumAmazonensis infectionInfecting doseProtective immunityChallenge infectionC57BL/6 miceAntibody responseCBA miceComplete adjuvantEffective adjuvantImmune responseImmunized animalsLog phase promastigotesLevel of protectionL. amazonensisInfectionC strainLeishmania amazonensisPurification and characterization of an 80-kilodalton membrane protein from Leishmania donovani
White A, McMahon-Pratt D. Purification and characterization of an 80-kilodalton membrane protein from Leishmania donovani. Infection And Immunity 1988, 56: 2385-2391. PMID: 3165961, PMCID: PMC259577, DOI: 10.1128/iai.56.9.2385-2391.1988.Peer-Reviewed Original Research
1987
Structural and antigenic characterization of a species- and promastigote-specific Leishmania mexicana amazonensis membrane protein.
Kahl L, McMahon-Pratt D. Structural and antigenic characterization of a species- and promastigote-specific Leishmania mexicana amazonensis membrane protein. The Journal Of Immunology 1987, 138: 1587-95. PMID: 3543130, DOI: 10.4049/jimmunol.138.5.1587.Peer-Reviewed Original ResearchConceptsMembrane proteinsIntegral membrane proteinsHydrophobic probe 3Secondary protein structurePeptide mapping experimentsProtein structureComponent polypeptidesParasite membraneLimited proteolysisMolecular massMembrane glycoproteinsAmino acidsDisulfide bondingMapping experimentsProteinProteolytic digestionHydrophobic interactionsProbe 3Large fragmentsPeptide fragmentsPromastigote membraneEnzymic cleavageEnzymic digestionFragmentsAntigenic characterization