1996
Leishmania amazonensis:Cultivation and Characterization of Axenic Amastigote-like Organisms
Hodgkinson V, Soong L, Duboise S, McMahon-Pratt D. Leishmania amazonensis:Cultivation and Characterization of Axenic Amastigote-like Organisms. Experimental Parasitology 1996, 83: 94-105. PMID: 8654556, DOI: 10.1006/expr.1996.0053.Peer-Reviewed Original ResearchConceptsAmastigote-like formsNorthern blot analysisDevelopmental biologyContinual cultureAxenic cultureMolecular biologyTransformation of promastigotesOrganismsDNA probesGeneration timeCultured amastigotesAxenic cultivationBlot analysisExpression levelsSpecies of LeishmaniaSpeciesProtein reactiveBiologyDegrees CFirst reportPeriod of growthAmastigotesPromastigotesLeishmania amazonensisCultivation
1993
Identification of two distinct cysteine proteinase genes of Leishmania pifanoi axenic amastigotes using the polymerase chain reaction
Traub-Cseko Y, Duboise M, Boukai L, McMahon-Pratt D. Identification of two distinct cysteine proteinase genes of Leishmania pifanoi axenic amastigotes using the polymerase chain reaction. Molecular And Biochemical Parasitology 1993, 57: 101-115. PMID: 8426606, DOI: 10.1016/0166-6851(93)90248-v.Peer-Reviewed Original ResearchConceptsC-terminal extensionCysteine proteinase geneComparative sequence analysisCysteine proteinasesActive site residuesAmastigote cysteine proteinasesMexicana complexChromosome locationGenomic arrangementDistinct genesCDNA sequenceSequence dataDNA sequencesTrypanosoma bruceiProtein sequencesBiological functionsProteinase geneSite residuesDegenerate primersSelective conservationLysosomal organellesGlycine residueGenomic copiesSequence analysisLeishmania mexicana complex
1991
Biochemical and molecular characterization ofLeishmania pifanoi amastigotes in continuous axenic culture
Rainey P, Spithill T, McMahon-Pratt D, Pan A. Biochemical and molecular characterization ofLeishmania pifanoi amastigotes in continuous axenic culture. Molecular And Biochemical Parasitology 1991, 49: 111-118. PMID: 1775152, DOI: 10.1016/0166-6851(91)90134-r.Peer-Reviewed Original ResearchConceptsContinuous axenic cultureSynthesis of DNAMolecular geneticsAdenine deaminaseAmastigote-like formsAxenic cultureAxenic mediumExcellent modelAxenic formsLeishmania pifanoiMetabolism of glucosePeroxidase activityAmastigote formsPromastigote formsAmastigotesCulture formsGenesGeneticsRNAProteinDNAReductaseRibonucleaseLinoleic acidPrevious demonstration