2000
The Immunologically Protective P-4 Antigen ofLeishmania Amastigotes A DEVELOPMENTALLY REGULATED SINGLE STRAND-SPECIFIC NUCLEASE ASSOCIATED WITH THE ENDOPLASMIC RETICULUM*
Kar S, Soong L, Colmenares M, Goldsmith-Pestana K, McMahon-Pratt D. The Immunologically Protective P-4 Antigen ofLeishmania Amastigotes A DEVELOPMENTALLY REGULATED SINGLE STRAND-SPECIFIC NUCLEASE ASSOCIATED WITH THE ENDOPLASMIC RETICULUM*. Journal Of Biological Chemistry 2000, 275: 37789-37797. PMID: 10969068, DOI: 10.1074/jbc.m002149200.Peer-Reviewed Original ResearchConceptsEndoplasmic reticulumSpecific nuclease activityBlot analysisImmunofluorescence microscopic analysisIntracellular amastigote stageProtein sequence dataSingle-strand nucleasePeripheral blood mononuclear cellsSouthern blot analysisNorthern blot analysisBlood mononuclear cellsMembrane proteinsRNA stabilitySubcellular localizationFuture vaccine studiesDNA repairSequence dataTerminal domainAmerican cutaneous leishmaniasisSequence analysisNuclease activityAmastigote stagePromastigote stageSpecific nucleasesT helper
1994
The biosynthesis, processing, and immunolocalization of Leishmania pifanoi amastigote cysteine proteinases
Duboise S, Vannier-Santos M, Costa-Pinto D, Rivas L, Pan A, Traub-Cseko Y, De Souza W, McMahon-Pratt D. The biosynthesis, processing, and immunolocalization of Leishmania pifanoi amastigote cysteine proteinases. Molecular And Biochemical Parasitology 1994, 68: 119-132. PMID: 7891736, DOI: 10.1016/0166-6851(94)00157-x.Peer-Reviewed Original ResearchConceptsTerminal domainMature proteinaseFlagellar pocketCysteine proteinasesParasite cell divisionCOOH-terminal domainProtein sequence dataAmastigote cysteine proteinasesElectron microscopic immunolocalizationLow-level secretionCell divisionSequence dataPlasma membraneIntracellular targetingLeishmania pifanoiProteolytic processingPulse labelingAcid proteinase inhibitorsProteinase inhibitorsCultured amastigotesLevel secretionL. pifanoiExtension domainBiosynthesisEnzymatic processing