2000
The Immunologically Protective P-4 Antigen ofLeishmania Amastigotes A DEVELOPMENTALLY REGULATED SINGLE STRAND-SPECIFIC NUCLEASE ASSOCIATED WITH THE ENDOPLASMIC RETICULUM*
Kar S, Soong L, Colmenares M, Goldsmith-Pestana K, McMahon-Pratt D. The Immunologically Protective P-4 Antigen ofLeishmania Amastigotes A DEVELOPMENTALLY REGULATED SINGLE STRAND-SPECIFIC NUCLEASE ASSOCIATED WITH THE ENDOPLASMIC RETICULUM*. Journal Of Biological Chemistry 2000, 275: 37789-37797. PMID: 10969068, DOI: 10.1074/jbc.m002149200.Peer-Reviewed Original ResearchConceptsEndoplasmic reticulumSpecific nuclease activityBlot analysisImmunofluorescence microscopic analysisIntracellular amastigote stageProtein sequence dataSingle-strand nucleasePeripheral blood mononuclear cellsSouthern blot analysisNorthern blot analysisBlood mononuclear cellsMembrane proteinsRNA stabilitySubcellular localizationFuture vaccine studiesDNA repairSequence dataTerminal domainAmerican cutaneous leishmaniasisSequence analysisNuclease activityAmastigote stagePromastigote stageSpecific nucleasesT helper
1987
Structural and antigenic characterization of a species- and promastigote-specific Leishmania mexicana amazonensis membrane protein.
Kahl L, McMahon-Pratt D. Structural and antigenic characterization of a species- and promastigote-specific Leishmania mexicana amazonensis membrane protein. The Journal Of Immunology 1987, 138: 1587-95. PMID: 3543130, DOI: 10.4049/jimmunol.138.5.1587.Peer-Reviewed Original ResearchConceptsMembrane proteinsIntegral membrane proteinsHydrophobic probe 3Secondary protein structurePeptide mapping experimentsProtein structureComponent polypeptidesParasite membraneLimited proteolysisMolecular massMembrane glycoproteinsAmino acidsDisulfide bondingMapping experimentsProteinProteolytic digestionHydrophobic interactionsProbe 3Large fragmentsPeptide fragmentsPromastigote membraneEnzymic cleavageEnzymic digestionFragmentsAntigenic characterization