1996
Leishmania amazonensis:Cultivation and Characterization of Axenic Amastigote-like Organisms
Hodgkinson V, Soong L, Duboise S, McMahon-Pratt D. Leishmania amazonensis:Cultivation and Characterization of Axenic Amastigote-like Organisms. Experimental Parasitology 1996, 83: 94-105. PMID: 8654556, DOI: 10.1006/expr.1996.0053.Peer-Reviewed Original ResearchConceptsAmastigote-like formsNorthern blot analysisDevelopmental biologyContinual cultureAxenic cultureMolecular biologyTransformation of promastigotesOrganismsDNA probesGeneration timeCultured amastigotesAxenic cultivationBlot analysisExpression levelsSpecies of LeishmaniaSpeciesProtein reactiveBiologyDegrees CFirst reportPeriod of growthAmastigotesPromastigotesLeishmania amazonensisCultivation
1993
Developmental Life Cycle of Leishmania—Cultivation and Characterization of Cultured Extracellular Amastigotes1
PAN A, DUBOISE S, EPERON S, RIVAS L, HODGKINSON V, TRAUB‐CSEKO Y, MCMAHON‐PRATT D. Developmental Life Cycle of Leishmania—Cultivation and Characterization of Cultured Extracellular Amastigotes1. Journal Of Eukaryotic Microbiology 1993, 40: 213-223. PMID: 8461895, DOI: 10.1111/j.1550-7408.1993.tb04906.x.Peer-Reviewed Reviews, Practice Guidelines, Standards, and Consensus StatementsConceptsPlethora of proteinsCultured amastigotesDevelopmental life cycleDevelopmental responsesDevelopmental regulationAxenic cultureAxenic linesAmastigote stageBiochemical evidenceLife cycleInfected macrophagesOrganismsEnvironmental parametersLeishmania promastigotesAmastigotesDevelopmental investigationsLeishmaniaProteinParasitesRegulationBiochemistryLinesLeishmania amastigotesCharacterizationLeishmania organisms
1991
Biochemical and molecular characterization ofLeishmania pifanoi amastigotes in continuous axenic culture
Rainey P, Spithill T, McMahon-Pratt D, Pan A. Biochemical and molecular characterization ofLeishmania pifanoi amastigotes in continuous axenic culture. Molecular And Biochemical Parasitology 1991, 49: 111-118. PMID: 1775152, DOI: 10.1016/0166-6851(91)90134-r.Peer-Reviewed Original ResearchConceptsContinuous axenic cultureSynthesis of DNAMolecular geneticsAdenine deaminaseAmastigote-like formsAxenic cultureAxenic mediumExcellent modelAxenic formsLeishmania pifanoiMetabolism of glucosePeroxidase activityAmastigote formsPromastigote formsAmastigotesCulture formsGenesGeneticsRNAProteinDNAReductaseRibonucleaseLinoleic acidPrevious demonstration
1989
Amastigote and epimastigote stage-specific components of Trypanosoma cruzi characterized by using monoclonal antibodies. Purification and molecular characterization of an 83-kilodalton amastigote protein.
Pan A, McMahon-Pratt D. Amastigote and epimastigote stage-specific components of Trypanosoma cruzi characterized by using monoclonal antibodies. Purification and molecular characterization of an 83-kilodalton amastigote protein. The Journal Of Immunology 1989, 143: 1001-8. PMID: 2501384, DOI: 10.4049/jimmunol.143.3.1001.Peer-Reviewed Original ResearchConceptsTissue culture-derived trypomastigotesTrypanosoma cruziCruzi amastigotesPreliminary sequence dataStage-specific componentsCulture-derived trypomastigotesStage-specific monoclonal antibodiesSingle protein moleculesMembrane-enriched preparationsAmino acid compositionSequence dataEpimastigote membranesHuman chagasic seraStage differentiationAxenic cultureMolecular characterizationT. cruzi amastigotesEpimastigotesProtein moleculesProteinChagas diseaseMonoclonal antibodiesLeishmania speciesAcid compositionChagasic sera