2024
An immunophenotype-coupled transcriptomic atlas of human hematopoietic progenitors
Zhang X, Song B, Carlino M, Li G, Ferchen K, Chen M, Thompson E, Kain B, Schnell D, Thakkar K, Kouril M, Jin K, Hay S, Sen S, Bernardicius D, Ma S, Bennett S, Croteau J, Salvatori O, Lye M, Gillen A, Jordan C, Singh H, Krause D, Salomonis N, Grimes H. An immunophenotype-coupled transcriptomic atlas of human hematopoietic progenitors. Nature Immunology 2024, 25: 703-715. PMID: 38514887, PMCID: PMC11003869, DOI: 10.1038/s41590-024-01782-4.Peer-Reviewed Original ResearchSurface markersLeukemia stem cell populationHematopoietic progenitor compartmentBone marrow cellsHuman bone marrow cellsHuman hematopoietic progenitorsCell surface markersStem cell populationCITE-seqClinical responseHematopoietic progenitorsMarrow cellsProgenitor compartmentCellular Indexing of TranscriptomesTransitional cellsCell populationsProgenitor analysisCellular indicesMultimodal approachGenomics programsProgenitor stateTranscriptome profilingSurface proteinsProgenitorsCell states
2023
3092 – CRISPR OPTIMIZATION TO SCREEN FOR GENES THAT REGULATE FATE SPECIFICATION OF PRIMARY HUMAN HEMATOPOIETIC PROGENITORS
Mancuso R, Thompson E, Wang L, Krause D. 3092 – CRISPR OPTIMIZATION TO SCREEN FOR GENES THAT REGULATE FATE SPECIFICATION OF PRIMARY HUMAN HEMATOPOIETIC PROGENITORS. Experimental Hematology 2023, 124: s96. DOI: 10.1016/j.exphem.2023.06.199.Peer-Reviewed Original ResearchFate specificationMCherry fluorescent reporterFluorescent reportersNumber of genesLarge-scale screenDNA-PK inhibitorPrimary human hematopoietic progenitorsHuman hematopoietic progenitorsCD45-negative cellsPanel of genesCRISPR screensErythroid maturationSingle guideMolecular mechanismsGRNAGRNA sequencesNegative cellsGenesHematopoietic progenitorsLentiviral transductionTotal RNAReporterProgenitorsTransfectionCell number
2022
Multiparameter analysis of timelapse imaging reveals kinetics of megakaryocytic erythroid progenitor clonal expansion and differentiation
Scanlon VM, Thompson EN, Lawton BR, Kochugaeva M, Ta K, Mayday MY, Xavier-Ferrucio J, Kang E, Eskow NM, Lu YC, Kwon N, Laumas A, Cenci M, Lawrence K, Barden K, Larsuel ST, Reed FE, Peña-Carmona G, Ubbelohde A, Lee JP, Boobalan S, Oppong Y, Anderson R, Maynard C, Sahirul K, Lajeune C, Ivathraya V, Addy T, Sanchez P, Holbrook C, Van Ho AT, Duncan JS, Blau HM, Levchenko A, Krause DS. Multiparameter analysis of timelapse imaging reveals kinetics of megakaryocytic erythroid progenitor clonal expansion and differentiation. Scientific Reports 2022, 12: 16218. PMID: 36171423, PMCID: PMC9519589, DOI: 10.1038/s41598-022-19013-x.Peer-Reviewed Original ResearchConceptsMegakaryocytic-erythroid progenitorsFate specificationLineage commitmentUnderstanding of hematopoiesisProgenitor cell biologyPrimary human hematopoietic progenitorsSingle-cell trackingSingle-cell assaysSingle-cell levelHuman hematopoietic progenitorsProgenitor cell dynamicsLineage specificationCell fateColony-forming unit assaysTimelapse imagingSitu fluorescence stainingCell biologyLineage tracingDivision rateCytokine thrombopoietinHematopoietic progenitorsProgenitorsFluorescence stainingCell dynamicsUnit assays
2018
Concise Review: Bipotent Megakaryocytic-Erythroid Progenitors: Concepts and Controversies
Xavier-Ferrucio J, Krause DS. Concise Review: Bipotent Megakaryocytic-Erythroid Progenitors: Concepts and Controversies. Stem Cells 2018, 36: 1138-1145. PMID: 29658164, PMCID: PMC6105498, DOI: 10.1002/stem.2834.Peer-Reviewed Original ResearchConceptsMegakaryocytic-erythroid progenitorsProgenitor cellsDifferent functional outputsVariety of speciesProgenitor stageIntermediate progenitor stageErythroid cellsHuman hematopoiesisBlood formationMegakaryocytic lineageMurine cellsHematopoietic stemHematopoietic progenitorsFunctional outputStem cellsDifferentiation capabilityHematopoiesis processProgenitorsLineagesHematopoiesisCell sourceCellsDiscrete stepsRecent advancesSpecies
2014
Nonstochastic Reprogramming from a Privileged Somatic Cell State
Guo S, Zi X, Schulz VP, Cheng J, Zhong M, Koochaki SH, Megyola CM, Pan X, Heydari K, Weissman SM, Gallagher PG, Krause DS, Fan R, Lu J. Nonstochastic Reprogramming from a Privileged Somatic Cell State. Cell 2014, 156: 649-662. PMID: 24486105, PMCID: PMC4318260, DOI: 10.1016/j.cell.2014.01.020.Peer-Reviewed Original ResearchConceptsSomatic cell stateCell statesAcquisition of pluripotencyMurine hematopoietic progenitorsEndogenous Oct4Cell cycle accelerationNonstochastic mannerSomatic cellsProgeny cellsPluripotent fateYamanaka factorsCell cycleHematopoietic progenitorsP53 knockdownPluripotencyReprogrammingCycling populationFactor expressionCellsFibroblastsImportant bottleneckKnockdownProgenitorsFateExpression
1994
Characterization of Murine CD34, a Marker for Hematopoietic Progenitor and Stem Cells
Krause D, Ito T, Fackler M, Smith O, Collector M, Sharkis S, May W. Characterization of Murine CD34, a Marker for Hematopoietic Progenitor and Stem Cells. Blood 1994, 84: 691-701. PMID: 7519070, DOI: 10.1182/blood.v84.3.691.691.Peer-Reviewed Original ResearchConceptsFull-length CD34Colony-forming unit-spleenM1 leukemia cellsMurine CD34Stem cellsHematopoietic progenitorsMurine hematopoietic stem cellsProgenitor cellsBone marrow cellsEmbryonic stem cellsHuman hematopoietic stemPotential functional differencesLong-term repopulating abilityCell linesFunctional hematopoietic progenitorsFull-length formCHO-K1 cell lineDifferent cell typesMurine cell linesPattern of expressionPA6 stromal cellsHematopoietic stem cellsLeukemia cellsHematopoietic progenitor cellsApparent molecular weightCharacterization of murine CD34, a marker for hematopoietic progenitor and stem cells
Krause D, Ito T, Fackler M, Smith O, Collector M, Sharkis S, May W. Characterization of murine CD34, a marker for hematopoietic progenitor and stem cells. Blood 1994, 84: 691-701. DOI: 10.1182/blood.v84.3.691.bloodjournal843691.Peer-Reviewed Original ResearchFull-length CD34Colony-forming unit-spleenM1 leukemia cellsMurine CD34Stem cellsHematopoietic progenitorsMurine hematopoietic stem cellsProgenitor cellsBone marrow cellsEmbryonic stem cellsHuman hematopoietic stemPotential functional differencesLong-term repopulating abilityCell linesFunctional hematopoietic progenitorsFull-length formCHO-K1 cell lineDifferent cell typesMurine cell linesPattern of expressionPA6 stromal cellsHematopoietic stem cellsLeukemia cellsHematopoietic progenitor cellsApparent molecular weight